Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IJEDHG_03245 and gcvP are separated by 263 nucleotides gcvP and gcvH are separated by 23 nucleotides gcvH and gcvT are separated by 75 nucleotides
IJEDHG_03245: IJEDHG_03245 - hypothetical protein, at 484,664 to 484,954
_03245
IJEDHG_03250: gcvP - aminomethyl-transferring glycine dehydrogenase, at 485,218 to 488,100
gcvP
IJEDHG_03255: gcvH - glycine cleavage system protein GcvH, at 488,124 to 488,510
gcvH
IJEDHG_03260: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 488,586 to 489,680
gcvT
Position (kb) 485
486
487
488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 484.289 kb on + strand at 484.290 kb on - strand at 484.337 kb on + strand at 484.361 kb on + strand at 484.362 kb on - strand at 484.362 kb on - strand at 484.362 kb on - strand at 484.396 kb on + strand at 484.397 kb on - strand at 484.397 kb on - strand at 484.437 kb on + strand at 484.437 kb on + strand at 484.437 kb on + strand at 484.529 kb on - strand at 484.601 kb on + strand at 484.602 kb on - strand at 484.609 kb on + strand at 484.610 kb on - strand at 484.610 kb on - strand at 484.611 kb on + strand at 484.612 kb on - strand at 484.690 kb on + strand at 484.691 kb on - strand at 484.703 kb on - strand, within IJEDHG_03245 at 484.811 kb on + strand, within IJEDHG_03245 at 484.812 kb on - strand, within IJEDHG_03245 at 484.825 kb on - strand, within IJEDHG_03245 at 484.918 kb on + strand, within IJEDHG_03245 at 484.919 kb on - strand, within IJEDHG_03245 at 485.015 kb on - strand at 485.097 kb on + strand at 485.168 kb on - strand at 485.312 kb on + strand at 485.313 kb on - strand at 485.313 kb on - strand at 485.313 kb on - strand at 485.316 kb on + strand at 485.316 kb on + strand at 485.317 kb on - strand at 485.355 kb on + strand at 485.356 kb on - strand at 485.655 kb on + strand, within gcvP at 485.655 kb on + strand, within gcvP at 485.655 kb on + strand, within gcvP at 485.692 kb on + strand, within gcvP at 485.730 kb on - strand, within gcvP at 485.730 kb on - strand, within gcvP at 485.795 kb on + strand, within gcvP at 485.796 kb on - strand, within gcvP at 485.796 kb on - strand, within gcvP at 485.924 kb on - strand, within gcvP at 485.979 kb on + strand, within gcvP at 486.014 kb on + strand, within gcvP at 486.014 kb on + strand, within gcvP at 486.015 kb on - strand, within gcvP at 486.048 kb on + strand, within gcvP at 486.066 kb on - strand, within gcvP at 486.123 kb on - strand, within gcvP at 486.154 kb on - strand, within gcvP at 486.371 kb on + strand, within gcvP at 486.372 kb on - strand, within gcvP at 486.416 kb on + strand, within gcvP at 486.468 kb on - strand, within gcvP at 486.525 kb on + strand, within gcvP at 486.525 kb on + strand, within gcvP at 486.525 kb on + strand, within gcvP at 486.630 kb on - strand, within gcvP at 486.630 kb on - strand, within gcvP at 486.786 kb on + strand, within gcvP at 486.796 kb on + strand, within gcvP at 486.804 kb on + strand, within gcvP at 486.805 kb on - strand, within gcvP at 486.805 kb on - strand, within gcvP at 486.851 kb on + strand, within gcvP at 486.852 kb on - strand, within gcvP at 486.852 kb on - strand, within gcvP at 487.059 kb on - strand, within gcvP at 487.095 kb on + strand, within gcvP at 487.102 kb on - strand, within gcvP at 487.354 kb on - strand, within gcvP at 487.703 kb on - strand, within gcvP at 487.757 kb on + strand, within gcvP at 487.850 kb on + strand at 487.968 kb on + strand at 487.968 kb on + strand at 487.968 kb on + strand at 487.969 kb on - strand at 488.046 kb on + strand at 488.118 kb on + strand at 488.135 kb on + strand at 488.136 kb on - strand at 488.268 kb on + strand, within gcvH at 488.269 kb on - strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.299 kb on + strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.300 kb on - strand, within gcvH at 488.320 kb on - strand, within gcvH at 488.357 kb on - strand, within gcvH at 488.357 kb on - strand, within gcvH at 488.499 kb on + strand at 488.499 kb on + strand at 488.499 kb on + strand at 488.500 kb on - strand at 488.500 kb on - strand at 488.536 kb on - strand at 488.588 kb on - strand at 488.655 kb on + strand at 488.655 kb on + strand at 488.656 kb on - strand at 488.656 kb on - strand at 488.709 kb on - strand, within gcvT at 488.780 kb on + strand, within gcvT at 488.893 kb on + strand, within gcvT at 488.893 kb on + strand, within gcvT at 488.974 kb on + strand, within gcvT
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 484,289 + -0.7 484,290 - -0.0 484,337 + +1.0 484,361 + -0.0 484,362 - -0.5 484,362 - +0.7 484,362 - -0.2 484,396 + -0.4 484,397 - -0.4 484,397 - +0.4 484,437 + +0.3 484,437 + +0.6 484,437 + -1.2 484,529 - +0.1 484,601 + +0.6 484,602 - -0.8 484,609 + +0.5 484,610 - +0.7 484,610 - -1.2 484,611 + -0.0 484,612 - +0.2 484,690 + -1.6 484,691 - -0.3 484,703 - IJEDHG_03245 0.13 -0.0 484,811 + IJEDHG_03245 0.51 -0.3 484,812 - IJEDHG_03245 0.51 +0.1 484,825 - IJEDHG_03245 0.55 -0.0 484,918 + IJEDHG_03245 0.87 -0.7 484,919 - IJEDHG_03245 0.88 -0.2 485,015 - +1.0 485,097 + +0.3 485,168 - -0.0 485,312 + +0.2 485,313 - -0.0 485,313 - -0.2 485,313 - -0.9 485,316 + -0.0 485,316 + -0.7 485,317 - +1.1 485,355 + -0.2 485,356 - -1.0 485,655 + gcvP IJEDHG_03250 0.15 -0.6 485,655 + gcvP IJEDHG_03250 0.15 -0.3 485,655 + gcvP IJEDHG_03250 0.15 +1.6 485,692 + gcvP IJEDHG_03250 0.16 -1.4 485,730 - gcvP IJEDHG_03250 0.18 -0.0 485,730 - gcvP IJEDHG_03250 0.18 -0.5 485,795 + gcvP IJEDHG_03250 0.20 +1.1 485,796 - gcvP IJEDHG_03250 0.20 -1.0 485,796 - gcvP IJEDHG_03250 0.20 -0.0 485,924 - gcvP IJEDHG_03250 0.24 -0.0 485,979 + gcvP IJEDHG_03250 0.26 -1.4 486,014 + gcvP IJEDHG_03250 0.28 -0.8 486,014 + gcvP IJEDHG_03250 0.28 -1.0 486,015 - gcvP IJEDHG_03250 0.28 +0.6 486,048 + gcvP IJEDHG_03250 0.29 -2.1 486,066 - gcvP IJEDHG_03250 0.29 -0.0 486,123 - gcvP IJEDHG_03250 0.31 -1.4 486,154 - gcvP IJEDHG_03250 0.32 -1.4 486,371 + gcvP IJEDHG_03250 0.40 -0.6 486,372 - gcvP IJEDHG_03250 0.40 -1.0 486,416 + gcvP IJEDHG_03250 0.42 -0.8 486,468 - gcvP IJEDHG_03250 0.43 -0.4 486,525 + gcvP IJEDHG_03250 0.45 -3.6 486,525 + gcvP IJEDHG_03250 0.45 -0.4 486,525 + gcvP IJEDHG_03250 0.45 -1.0 486,630 - gcvP IJEDHG_03250 0.49 +0.4 486,630 - gcvP IJEDHG_03250 0.49 -1.0 486,786 + gcvP IJEDHG_03250 0.54 -2.3 486,796 + gcvP IJEDHG_03250 0.55 -0.4 486,804 + gcvP IJEDHG_03250 0.55 -0.0 486,805 - gcvP IJEDHG_03250 0.55 -0.8 486,805 - gcvP IJEDHG_03250 0.55 -0.0 486,851 + gcvP IJEDHG_03250 0.57 -1.2 486,852 - gcvP IJEDHG_03250 0.57 -1.0 486,852 - gcvP IJEDHG_03250 0.57 -1.6 487,059 - gcvP IJEDHG_03250 0.64 -1.2 487,095 + gcvP IJEDHG_03250 0.65 +0.4 487,102 - gcvP IJEDHG_03250 0.65 -0.9 487,354 - gcvP IJEDHG_03250 0.74 +1.3 487,703 - gcvP IJEDHG_03250 0.86 -0.4 487,757 + gcvP IJEDHG_03250 0.88 -1.3 487,850 + -1.0 487,968 + -0.0 487,968 + -1.3 487,968 + -0.4 487,969 - +0.2 488,046 + -0.0 488,118 + -0.9 488,135 + +0.4 488,136 - +1.6 488,268 + gcvH IJEDHG_03255 0.37 -2.0 488,269 - gcvH IJEDHG_03255 0.37 -1.3 488,299 + gcvH IJEDHG_03255 0.45 -0.7 488,299 + gcvH IJEDHG_03255 0.45 -0.0 488,299 + gcvH IJEDHG_03255 0.45 -0.4 488,299 + gcvH IJEDHG_03255 0.45 -0.9 488,299 + gcvH IJEDHG_03255 0.45 -0.0 488,299 + gcvH IJEDHG_03255 0.45 -0.5 488,299 + gcvH IJEDHG_03255 0.45 -1.5 488,299 + gcvH IJEDHG_03255 0.45 -0.0 488,299 + gcvH IJEDHG_03255 0.45 -0.7 488,299 + gcvH IJEDHG_03255 0.45 +0.4 488,300 - gcvH IJEDHG_03255 0.45 -0.4 488,300 - gcvH IJEDHG_03255 0.45 +0.7 488,300 - gcvH IJEDHG_03255 0.45 -1.8 488,300 - gcvH IJEDHG_03255 0.45 +0.2 488,300 - gcvH IJEDHG_03255 0.45 -0.2 488,300 - gcvH IJEDHG_03255 0.45 -1.6 488,300 - gcvH IJEDHG_03255 0.45 -0.3 488,320 - gcvH IJEDHG_03255 0.51 -0.2 488,357 - gcvH IJEDHG_03255 0.60 -0.0 488,357 - gcvH IJEDHG_03255 0.60 +0.2 488,499 + -0.5 488,499 + -1.6 488,499 + +0.5 488,500 - -1.0 488,500 - -1.1 488,536 - -1.3 488,588 - +0.4 488,655 + -0.0 488,655 + -1.5 488,656 - -2.2 488,656 - -0.4 488,709 - gcvT IJEDHG_03260 0.11 -2.2 488,780 + gcvT IJEDHG_03260 0.18 +0.6 488,893 + gcvT IJEDHG_03260 0.28 -0.8 488,893 + gcvT IJEDHG_03260 0.28 -0.6 488,974 + gcvT IJEDHG_03260 0.35 -0.6
Or see this region's nucleotide sequence