Experiment: 0.1X LB plate, 1:1 coculture with Promicromonospora thailandica SAI_039
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00872 and MPMX20_00873 are separated by 28 nucleotides MPMX20_00873 and MPMX20_00874 overlap by 14 nucleotides MPMX20_00874 and MPMX20_00875 are separated by 196 nucleotides MPMX20_00875 and MPMX20_00876 overlap by 4 nucleotides MPMX20_00876 and MPMX20_00877 are separated by 33 nucleotides
MPMX20_00872: MPMX20_00872 - tRNA(Ile)-lysidine synthase, at 923,897 to 925,183
_00872
MPMX20_00873: MPMX20_00873 - Protein rof, at 925,212 to 925,472
_00873
MPMX20_00874: MPMX20_00874 - hypothetical protein, at 925,459 to 925,659
_00874
MPMX20_00875: MPMX20_00875 - putative protein YaeQ, at 925,856 to 926,401
_00875
MPMX20_00876: MPMX20_00876 - Peptidyl-tRNA hydrolase ArfB, at 926,398 to 926,820
_00876
MPMX20_00877: MPMX20_00877 - Lipoprotein NlpE, at 926,854 to 927,552
_00877
Position (kb) 925
926
927 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 925.186 kb on - strand at 925.232 kb on - strand at 925.348 kb on + strand, within MPMX20_00873 at 925.391 kb on + strand, within MPMX20_00873 at 925.391 kb on + strand, within MPMX20_00873 at 925.399 kb on - strand, within MPMX20_00873 at 925.535 kb on - strand, within MPMX20_00874 at 925.539 kb on + strand, within MPMX20_00874 at 925.585 kb on + strand, within MPMX20_00874 at 925.585 kb on + strand, within MPMX20_00874 at 925.597 kb on + strand, within MPMX20_00874 at 925.597 kb on + strand, within MPMX20_00874 at 925.597 kb on + strand, within MPMX20_00874 at 925.605 kb on - strand, within MPMX20_00874 at 925.664 kb on + strand at 925.672 kb on - strand at 925.672 kb on - strand at 925.687 kb on - strand at 925.689 kb on - strand at 925.706 kb on - strand at 925.721 kb on - strand at 925.757 kb on - strand at 925.757 kb on - strand at 925.799 kb on - strand at 925.834 kb on - strand at 925.846 kb on + strand at 925.926 kb on - strand, within MPMX20_00875 at 925.926 kb on - strand, within MPMX20_00875 at 926.073 kb on + strand, within MPMX20_00875 at 926.151 kb on + strand, within MPMX20_00875 at 926.223 kb on - strand, within MPMX20_00875 at 926.271 kb on + strand, within MPMX20_00875 at 926.319 kb on + strand, within MPMX20_00875 at 926.331 kb on + strand, within MPMX20_00875 at 926.416 kb on + strand at 926.476 kb on + strand, within MPMX20_00876 at 926.570 kb on + strand, within MPMX20_00876 at 926.584 kb on - strand, within MPMX20_00876 at 926.617 kb on + strand, within MPMX20_00876 at 926.622 kb on + strand, within MPMX20_00876 at 926.730 kb on - strand, within MPMX20_00876 at 926.735 kb on + strand, within MPMX20_00876 at 926.745 kb on + strand, within MPMX20_00876 at 926.871 kb on - strand at 926.990 kb on + strand, within MPMX20_00877 at 927.000 kb on - strand, within MPMX20_00877 at 927.000 kb on - strand, within MPMX20_00877 at 927.076 kb on - strand, within MPMX20_00877 at 927.123 kb on - strand, within MPMX20_00877 at 927.195 kb on - strand, within MPMX20_00877 at 927.242 kb on + strand, within MPMX20_00877 at 927.250 kb on - strand, within MPMX20_00877 at 927.381 kb on + strand, within MPMX20_00877 at 927.381 kb on + strand, within MPMX20_00877
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Promicromonospora thailandica SAI_039 remove 925,186 - +0.8 925,232 - +0.0 925,348 + MPMX20_00873 0.52 -0.7 925,391 + MPMX20_00873 0.69 -1.2 925,391 + MPMX20_00873 0.69 -0.4 925,399 - MPMX20_00873 0.72 +0.6 925,535 - MPMX20_00874 0.38 -0.9 925,539 + MPMX20_00874 0.40 +2.9 925,585 + MPMX20_00874 0.63 +1.0 925,585 + MPMX20_00874 0.63 -1.5 925,597 + MPMX20_00874 0.69 -1.4 925,597 + MPMX20_00874 0.69 -1.0 925,597 + MPMX20_00874 0.69 -0.8 925,605 - MPMX20_00874 0.73 -2.1 925,664 + +1.0 925,672 - +0.2 925,672 - -0.4 925,687 - +0.2 925,689 - +0.9 925,706 - +0.4 925,721 - +0.4 925,757 - +0.3 925,757 - -1.2 925,799 - +0.8 925,834 - +0.3 925,846 + +0.3 925,926 - MPMX20_00875 0.13 -1.4 925,926 - MPMX20_00875 0.13 +0.8 926,073 + MPMX20_00875 0.40 +0.2 926,151 + MPMX20_00875 0.54 -0.7 926,223 - MPMX20_00875 0.67 +4.6 926,271 + MPMX20_00875 0.76 +0.0 926,319 + MPMX20_00875 0.85 -0.3 926,331 + MPMX20_00875 0.87 +0.8 926,416 + -0.7 926,476 + MPMX20_00876 0.18 -0.7 926,570 + MPMX20_00876 0.41 +0.5 926,584 - MPMX20_00876 0.44 -2.3 926,617 + MPMX20_00876 0.52 -2.2 926,622 + MPMX20_00876 0.53 -0.0 926,730 - MPMX20_00876 0.78 +0.8 926,735 + MPMX20_00876 0.80 +0.9 926,745 + MPMX20_00876 0.82 +0.8 926,871 - -1.8 926,990 + MPMX20_00877 0.19 +0.6 927,000 - MPMX20_00877 0.21 +1.2 927,000 - MPMX20_00877 0.21 -0.8 927,076 - MPMX20_00877 0.32 +0.7 927,123 - MPMX20_00877 0.38 +0.8 927,195 - MPMX20_00877 0.49 +1.1 927,242 + MPMX20_00877 0.56 -0.9 927,250 - MPMX20_00877 0.57 -1.1 927,381 + MPMX20_00877 0.75 +3.2 927,381 + MPMX20_00877 0.75 -1.7
Or see this region's nucleotide sequence