Experiment: M9 plate, 1:1 coculture with Amycolatopsis sp. SAI_101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04463 and MPMX20_04464 are separated by 80 nucleotides MPMX20_04464 and MPMX20_04465 overlap by 1 nucleotides MPMX20_04465 and MPMX20_04466 overlap by 4 nucleotides
MPMX20_04463: MPMX20_04463 - DNA helicase II, at 4,666,589 to 4,668,751
_04463
MPMX20_04464: MPMX20_04464 - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 4,668,832 to 4,669,548
_04464
MPMX20_04465: MPMX20_04465 - Tyrosine recombinase XerC, at 4,669,548 to 4,670,450
_04465
MPMX20_04466: MPMX20_04466 - hypothetical protein, at 4,670,447 to 4,671,154
_04466
Position (kb)
4668
4669
4670 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4667.963 kb on + strand, within MPMX20_04463 at 4668.018 kb on - strand, within MPMX20_04463 at 4668.026 kb on - strand, within MPMX20_04463 at 4668.053 kb on + strand, within MPMX20_04463 at 4668.120 kb on + strand, within MPMX20_04463 at 4668.181 kb on + strand, within MPMX20_04463 at 4668.199 kb on - strand, within MPMX20_04463 at 4668.199 kb on - strand, within MPMX20_04463 at 4668.199 kb on - strand, within MPMX20_04463 at 4668.214 kb on + strand, within MPMX20_04463 at 4668.222 kb on - strand, within MPMX20_04463 at 4668.400 kb on - strand, within MPMX20_04463 at 4668.400 kb on - strand, within MPMX20_04463 at 4668.599 kb on + strand at 4668.668 kb on - strand at 4668.736 kb on + strand at 4668.879 kb on + strand at 4668.910 kb on - strand, within MPMX20_04464 at 4668.989 kb on - strand, within MPMX20_04464 at 4669.122 kb on + strand, within MPMX20_04464 at 4669.130 kb on - strand, within MPMX20_04464 at 4669.251 kb on + strand, within MPMX20_04464 at 4669.251 kb on + strand, within MPMX20_04464 at 4669.259 kb on - strand, within MPMX20_04464 at 4669.259 kb on - strand, within MPMX20_04464 at 4669.259 kb on - strand, within MPMX20_04464 at 4669.284 kb on + strand, within MPMX20_04464 at 4669.324 kb on - strand, within MPMX20_04464 at 4669.356 kb on - strand, within MPMX20_04464 at 4669.369 kb on + strand, within MPMX20_04464 at 4669.408 kb on + strand, within MPMX20_04464 at 4669.439 kb on - strand, within MPMX20_04464 at 4669.457 kb on + strand, within MPMX20_04464 at 4669.470 kb on - strand, within MPMX20_04464 at 4669.508 kb on - strand at 4669.508 kb on - strand at 4669.508 kb on - strand at 4669.520 kb on - strand at 4669.534 kb on - strand at 4669.618 kb on + strand at 4669.618 kb on + strand at 4669.626 kb on - strand at 4669.650 kb on + strand, within MPMX20_04465 at 4669.658 kb on - strand, within MPMX20_04465 at 4669.743 kb on + strand, within MPMX20_04465 at 4669.769 kb on - strand, within MPMX20_04465 at 4669.769 kb on - strand, within MPMX20_04465 at 4669.774 kb on + strand, within MPMX20_04465 at 4669.787 kb on - strand, within MPMX20_04465 at 4669.851 kb on - strand, within MPMX20_04465 at 4669.862 kb on + strand, within MPMX20_04465 at 4669.862 kb on + strand, within MPMX20_04465 at 4669.867 kb on + strand, within MPMX20_04465 at 4669.870 kb on - strand, within MPMX20_04465 at 4669.875 kb on - strand, within MPMX20_04465 at 4669.927 kb on + strand, within MPMX20_04465 at 4669.999 kb on - strand, within MPMX20_04465 at 4669.999 kb on - strand, within MPMX20_04465 at 4669.999 kb on - strand, within MPMX20_04465 at 4670.022 kb on - strand, within MPMX20_04465 at 4670.022 kb on - strand, within MPMX20_04465 at 4670.040 kb on + strand, within MPMX20_04465 at 4670.136 kb on - strand, within MPMX20_04465 at 4670.169 kb on - strand, within MPMX20_04465 at 4670.180 kb on - strand, within MPMX20_04465 at 4670.197 kb on + strand, within MPMX20_04465 at 4670.240 kb on - strand, within MPMX20_04465 at 4670.371 kb on + strand at 4670.385 kb on - strand at 4670.404 kb on - strand at 4670.404 kb on - strand at 4670.404 kb on - strand at 4670.481 kb on + strand at 4670.493 kb on - strand at 4670.518 kb on - strand, within MPMX20_04466 at 4670.535 kb on - strand, within MPMX20_04466
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Amycolatopsis sp. SAI_101 remove 4,667,963 + MPMX20_04463 0.64 +0.3 4,668,018 - MPMX20_04463 0.66 +0.1 4,668,026 - MPMX20_04463 0.66 -3.1 4,668,053 + MPMX20_04463 0.68 -0.3 4,668,120 + MPMX20_04463 0.71 -0.4 4,668,181 + MPMX20_04463 0.74 -0.9 4,668,199 - MPMX20_04463 0.74 -2.4 4,668,199 - MPMX20_04463 0.74 -1.3 4,668,199 - MPMX20_04463 0.74 +0.3 4,668,214 + MPMX20_04463 0.75 +0.6 4,668,222 - MPMX20_04463 0.75 -0.7 4,668,400 - MPMX20_04463 0.84 -1.7 4,668,400 - MPMX20_04463 0.84 -0.4 4,668,599 + -3.3 4,668,668 - -2.2 4,668,736 + -2.3 4,668,879 + -0.2 4,668,910 - MPMX20_04464 0.11 -1.1 4,668,989 - MPMX20_04464 0.22 -1.0 4,669,122 + MPMX20_04464 0.40 -1.3 4,669,130 - MPMX20_04464 0.42 -1.1 4,669,251 + MPMX20_04464 0.58 -0.3 4,669,251 + MPMX20_04464 0.58 -1.6 4,669,259 - MPMX20_04464 0.60 -0.1 4,669,259 - MPMX20_04464 0.60 -0.4 4,669,259 - MPMX20_04464 0.60 -0.7 4,669,284 + MPMX20_04464 0.63 -0.3 4,669,324 - MPMX20_04464 0.69 -0.8 4,669,356 - MPMX20_04464 0.73 -0.6 4,669,369 + MPMX20_04464 0.75 -0.0 4,669,408 + MPMX20_04464 0.80 -1.1 4,669,439 - MPMX20_04464 0.85 -0.1 4,669,457 + MPMX20_04464 0.87 +0.5 4,669,470 - MPMX20_04464 0.89 -1.6 4,669,508 - +0.5 4,669,508 - -1.6 4,669,508 - +0.2 4,669,520 - +1.0 4,669,534 - +0.1 4,669,618 + -1.4 4,669,618 + -0.1 4,669,626 - -2.1 4,669,650 + MPMX20_04465 0.11 -1.7 4,669,658 - MPMX20_04465 0.12 -1.8 4,669,743 + MPMX20_04465 0.22 -0.7 4,669,769 - MPMX20_04465 0.24 -2.5 4,669,769 - MPMX20_04465 0.24 -0.7 4,669,774 + MPMX20_04465 0.25 -0.7 4,669,787 - MPMX20_04465 0.26 +0.9 4,669,851 - MPMX20_04465 0.34 -0.4 4,669,862 + MPMX20_04465 0.35 -1.8 4,669,862 + MPMX20_04465 0.35 -1.7 4,669,867 + MPMX20_04465 0.35 -0.4 4,669,870 - MPMX20_04465 0.36 -1.3 4,669,875 - MPMX20_04465 0.36 -1.0 4,669,927 + MPMX20_04465 0.42 +1.9 4,669,999 - MPMX20_04465 0.50 -1.3 4,669,999 - MPMX20_04465 0.50 -1.4 4,669,999 - MPMX20_04465 0.50 -1.4 4,670,022 - MPMX20_04465 0.52 -1.3 4,670,022 - MPMX20_04465 0.52 -0.1 4,670,040 + MPMX20_04465 0.54 -2.6 4,670,136 - MPMX20_04465 0.65 -0.7 4,670,169 - MPMX20_04465 0.69 -0.6 4,670,180 - MPMX20_04465 0.70 +0.6 4,670,197 + MPMX20_04465 0.72 -0.1 4,670,240 - MPMX20_04465 0.77 -0.3 4,670,371 + +0.3 4,670,385 - -1.1 4,670,404 - +0.3 4,670,404 - -1.7 4,670,404 - -0.7 4,670,481 + -3.0 4,670,493 - +1.9 4,670,518 - MPMX20_04466 0.10 -3.4 4,670,535 - MPMX20_04466 0.12 -0.6
Or see this region's nucleotide sequence