Experiment: 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03906 and MPMX20_03907 are separated by 236 nucleotides MPMX20_03907 and MPMX20_03908 are separated by 114 nucleotides MPMX20_03908 and MPMX20_03909 are separated by 150 nucleotides
MPMX20_03906: MPMX20_03906 - tRNA N6-adenosine threonylcarbamoyltransferase, at 4,114,670 to 4,115,683
_03906
MPMX20_03907: MPMX20_03907 - 30S ribosomal protein S21, at 4,115,920 to 4,116,135
_03907
MPMX20_03908: MPMX20_03908 - DNA primase, at 4,116,250 to 4,117,995
_03908
MPMX20_03909: MPMX20_03909 - RNA polymerase sigma factor RpoD, at 4,118,146 to 4,119,993
_03909
Position (kb)
4116
4117
4118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4115.700 kb on + strand at 4115.708 kb on - strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.715 kb on + strand at 4115.721 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.723 kb on - strand at 4115.765 kb on + strand at 4115.769 kb on + strand at 4115.769 kb on + strand at 4115.769 kb on + strand at 4115.769 kb on + strand at 4115.777 kb on + strand at 4115.777 kb on - strand at 4115.777 kb on - strand at 4115.777 kb on - strand at 4115.777 kb on - strand at 4115.779 kb on + strand at 4115.779 kb on + strand at 4115.779 kb on + strand at 4115.785 kb on - strand at 4115.786 kb on - strand at 4115.787 kb on - strand at 4116.157 kb on + strand at 4118.041 kb on + strand at 4118.045 kb on + strand at 4118.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196 remove 4,115,700 + +1.0 4,115,708 - +0.2 4,115,715 + -1.0 4,115,715 + -0.7 4,115,715 + +0.5 4,115,715 + +1.5 4,115,715 + +1.2 4,115,715 + +0.9 4,115,715 + +0.5 4,115,715 + -0.8 4,115,715 + +0.2 4,115,715 + -0.3 4,115,715 + +0.5 4,115,715 + +1.3 4,115,721 - -0.8 4,115,723 - +0.3 4,115,723 - -1.7 4,115,723 - -1.0 4,115,723 - +3.2 4,115,723 - -0.7 4,115,723 - -0.7 4,115,723 - -2.2 4,115,723 - -2.1 4,115,723 - +0.5 4,115,723 - -0.4 4,115,723 - +1.2 4,115,723 - +2.7 4,115,723 - +0.2 4,115,723 - -1.5 4,115,723 - -2.1 4,115,723 - -2.8 4,115,723 - +0.1 4,115,723 - -1.6 4,115,723 - +0.4 4,115,765 + -2.3 4,115,769 + -1.7 4,115,769 + -0.4 4,115,769 + +0.8 4,115,769 + -0.6 4,115,777 + -3.3 4,115,777 - +1.1 4,115,777 - -0.3 4,115,777 - -0.7 4,115,777 - +1.6 4,115,779 + -1.9 4,115,779 + -0.3 4,115,779 + -1.6 4,115,785 - +0.0 4,115,786 - -1.7 4,115,787 - +0.2 4,116,157 + +0.2 4,118,041 + +0.5 4,118,045 + +0.5 4,118,083 + +2.5
Or see this region's nucleotide sequence