Experiment: 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01296 and MPMX20_01297 are separated by 270 nucleotides MPMX20_01297 and MPMX20_01298 are separated by 1 nucleotides MPMX20_01298 and MPMX20_01299 overlap by 1 nucleotides MPMX20_01299 and MPMX20_01300 are separated by 14 nucleotides
MPMX20_01296: MPMX20_01296 - Ribosomal silencing factor RsfS, at 1,357,331 to 1,357,648
_01296
MPMX20_01297: MPMX20_01297 - Nicotinate-nucleotide adenylyltransferase, at 1,357,919 to 1,358,575
_01297
MPMX20_01298: MPMX20_01298 - DNA polymerase III subunit delta, at 1,358,577 to 1,359,608
_01298
MPMX20_01299: MPMX20_01299 - LPS-assembly lipoprotein LptE, at 1,359,608 to 1,360,177
_01299
MPMX20_01300: MPMX20_01300 - Leucine--tRNA ligase, at 1,360,192 to 1,362,774
_01300
Position (kb)
1358
1359
1360 Strain fitness (log2 ratio)
-2
-1
0
1 at 1357.593 kb on - strand, within MPMX20_01296 at 1357.593 kb on - strand, within MPMX20_01296 at 1357.594 kb on - strand, within MPMX20_01296 at 1357.594 kb on - strand, within MPMX20_01296 at 1357.643 kb on - strand at 1357.643 kb on - strand at 1357.643 kb on - strand at 1357.669 kb on - strand at 1357.700 kb on + strand at 1357.710 kb on - strand at 1357.713 kb on - strand at 1357.713 kb on - strand at 1357.767 kb on + strand at 1357.775 kb on - strand at 1357.851 kb on + strand at 1357.853 kb on + strand at 1357.853 kb on + strand at 1357.859 kb on - strand at 1357.861 kb on - strand at 1357.861 kb on - strand at 1357.861 kb on - strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.865 kb on + strand at 1357.867 kb on - strand at 1357.867 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.873 kb on - strand at 1357.962 kb on - strand at 1358.614 kb on - strand at 1358.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196 remove 1,357,593 - MPMX20_01296 0.82 -1.7 1,357,593 - MPMX20_01296 0.82 +0.5 1,357,594 - MPMX20_01296 0.83 -1.2 1,357,594 - MPMX20_01296 0.83 -1.8 1,357,643 - -0.7 1,357,643 - -2.1 1,357,643 - +1.4 1,357,669 - -0.4 1,357,700 + +1.5 1,357,710 - +0.2 1,357,713 - +0.4 1,357,713 - -0.6 1,357,767 + -2.5 1,357,775 - -2.2 1,357,851 + +1.2 1,357,853 + -1.4 1,357,853 + -1.5 1,357,859 - -2.1 1,357,861 - +0.7 1,357,861 - -0.2 1,357,861 - -1.9 1,357,865 + -1.8 1,357,865 + -2.3 1,357,865 + -1.2 1,357,865 + +0.2 1,357,865 + +0.3 1,357,865 + +0.5 1,357,867 - -1.2 1,357,867 - -0.3 1,357,873 - +1.2 1,357,873 - -1.2 1,357,873 - -0.0 1,357,873 - -0.1 1,357,873 - +1.0 1,357,962 - +0.2 1,358,614 - -1.8 1,358,624 - +0.3
Or see this region's nucleotide sequence