Experiment: 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00866 and MPMX20_00867 overlap by 4 nucleotides MPMX20_00867 and MPMX20_00868 are separated by 38 nucleotides MPMX20_00868 and MPMX20_00869 are separated by 12 nucleotides
MPMX20_00866: MPMX20_00866 - Lipid-A-disaccharide synthase, at 914,917 to 916,065
_00866
MPMX20_00867: MPMX20_00867 - Ribonuclease HII, at 916,062 to 916,658
_00867
MPMX20_00868: MPMX20_00868 - DNA polymerase III subunit alpha, at 916,697 to 920,179
_00868
MPMX20_00869: MPMX20_00869 - Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, at 920,192 to 921,151
_00869
Position (kb) 916
917
918
919
920
921 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 916.043 kb on + strand at 916.045 kb on + strand at 916.045 kb on + strand at 916.232 kb on + strand, within MPMX20_00867 at 916.259 kb on + strand, within MPMX20_00867 at 916.259 kb on + strand, within MPMX20_00867 at 916.267 kb on + strand, within MPMX20_00867 at 916.318 kb on + strand, within MPMX20_00867 at 916.427 kb on + strand, within MPMX20_00867 at 916.434 kb on + strand, within MPMX20_00867 at 916.511 kb on + strand, within MPMX20_00867 at 916.517 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867 at 916.544 kb on + strand, within MPMX20_00867
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196 remove 916,043 + -1.1 916,045 + -2.5 916,045 + -0.7 916,232 + MPMX20_00867 0.28 -0.6 916,259 + MPMX20_00867 0.33 -1.2 916,259 + MPMX20_00867 0.33 +1.7 916,267 + MPMX20_00867 0.34 -0.2 916,318 + MPMX20_00867 0.43 -1.0 916,427 + MPMX20_00867 0.61 -3.2 916,434 + MPMX20_00867 0.62 +1.0 916,511 + MPMX20_00867 0.75 -1.7 916,517 + MPMX20_00867 0.76 -0.8 916,544 + MPMX20_00867 0.81 +1.5 916,544 + MPMX20_00867 0.81 +0.8 916,544 + MPMX20_00867 0.81 -0.8 916,544 + MPMX20_00867 0.81 -0.3 916,544 + MPMX20_00867 0.81 -0.1 916,544 + MPMX20_00867 0.81 -1.3 916,544 + MPMX20_00867 0.81 +1.9
Or see this region's nucleotide sequence