Experiment: 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00083 and MPMX20_00084 are separated by 2 nucleotides MPMX20_00084 and MPMX20_00085 are separated by 3 nucleotides MPMX20_00085 and MPMX20_00086 are separated by 4 nucleotides
MPMX20_00083: MPMX20_00083 - Sodium/glutamate symporter, at 84,752 to 85,954
_00083
MPMX20_00084: MPMX20_00084 - ATP-dependent DNA helicase RecG, at 85,957 to 88,038
_00084
MPMX20_00085: MPMX20_00085 - tRNA (guanosine(18)-2'-O)-methyltransferase, at 88,042 to 88,731
_00085
MPMX20_00086: MPMX20_00086 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 88,736 to 90,850
_00086
Position (kb) 85
86
87
88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 84.981 kb on + strand, within MPMX20_00083 at 84.981 kb on + strand, within MPMX20_00083 at 84.989 kb on - strand, within MPMX20_00083 at 84.989 kb on - strand, within MPMX20_00083 at 85.043 kb on - strand, within MPMX20_00083 at 85.043 kb on - strand, within MPMX20_00083 at 85.186 kb on - strand, within MPMX20_00083 at 85.189 kb on + strand, within MPMX20_00083 at 85.237 kb on + strand, within MPMX20_00083 at 85.237 kb on + strand, within MPMX20_00083 at 85.259 kb on + strand, within MPMX20_00083 at 85.262 kb on - strand, within MPMX20_00083 at 85.381 kb on + strand, within MPMX20_00083 at 85.381 kb on + strand, within MPMX20_00083 at 85.439 kb on - strand, within MPMX20_00083 at 85.486 kb on + strand, within MPMX20_00083 at 85.490 kb on + strand, within MPMX20_00083 at 85.624 kb on + strand, within MPMX20_00083 at 85.629 kb on + strand, within MPMX20_00083 at 85.656 kb on - strand, within MPMX20_00083 at 85.696 kb on + strand, within MPMX20_00083 at 85.713 kb on - strand, within MPMX20_00083 at 85.787 kb on + strand, within MPMX20_00083 at 85.879 kb on - strand at 85.931 kb on - strand at 86.024 kb on - strand at 86.024 kb on - strand at 86.024 kb on - strand at 86.316 kb on + strand, within MPMX20_00084 at 86.372 kb on - strand, within MPMX20_00084 at 86.461 kb on - strand, within MPMX20_00084 at 86.477 kb on + strand, within MPMX20_00084 at 86.525 kb on + strand, within MPMX20_00084 at 86.547 kb on + strand, within MPMX20_00084 at 86.555 kb on - strand, within MPMX20_00084 at 86.584 kb on - strand, within MPMX20_00084 at 86.584 kb on - strand, within MPMX20_00084 at 86.654 kb on - strand, within MPMX20_00084 at 86.670 kb on + strand, within MPMX20_00084 at 86.738 kb on - strand, within MPMX20_00084 at 86.753 kb on - strand, within MPMX20_00084 at 86.798 kb on - strand, within MPMX20_00084 at 86.831 kb on - strand, within MPMX20_00084 at 86.839 kb on + strand, within MPMX20_00084 at 86.880 kb on + strand, within MPMX20_00084 at 86.949 kb on + strand, within MPMX20_00084 at 86.962 kb on + strand, within MPMX20_00084 at 86.987 kb on + strand, within MPMX20_00084 at 86.987 kb on + strand, within MPMX20_00084 at 86.997 kb on + strand, within MPMX20_00084 at 87.020 kb on - strand, within MPMX20_00084 at 87.037 kb on + strand, within MPMX20_00084 at 87.040 kb on + strand, within MPMX20_00084 at 87.101 kb on - strand, within MPMX20_00084 at 87.151 kb on + strand, within MPMX20_00084 at 87.219 kb on + strand, within MPMX20_00084 at 87.284 kb on + strand, within MPMX20_00084 at 87.292 kb on - strand, within MPMX20_00084 at 87.339 kb on + strand, within MPMX20_00084 at 87.363 kb on + strand, within MPMX20_00084 at 87.371 kb on - strand, within MPMX20_00084 at 87.404 kb on + strand, within MPMX20_00084 at 87.411 kb on + strand, within MPMX20_00084 at 87.441 kb on - strand, within MPMX20_00084 at 87.441 kb on - strand, within MPMX20_00084 at 87.457 kb on - strand, within MPMX20_00084 at 87.463 kb on + strand, within MPMX20_00084 at 87.463 kb on + strand, within MPMX20_00084 at 87.464 kb on + strand, within MPMX20_00084 at 87.493 kb on - strand, within MPMX20_00084 at 87.511 kb on + strand, within MPMX20_00084 at 87.552 kb on + strand, within MPMX20_00084 at 87.609 kb on + strand, within MPMX20_00084 at 87.617 kb on - strand, within MPMX20_00084 at 87.627 kb on - strand, within MPMX20_00084 at 87.689 kb on + strand, within MPMX20_00084 at 87.694 kb on + strand, within MPMX20_00084 at 87.694 kb on + strand, within MPMX20_00084 at 87.694 kb on + strand, within MPMX20_00084 at 87.697 kb on - strand, within MPMX20_00084 at 87.697 kb on - strand, within MPMX20_00084 at 87.702 kb on + strand, within MPMX20_00084 at 87.702 kb on - strand, within MPMX20_00084 at 87.702 kb on - strand, within MPMX20_00084 at 87.792 kb on - strand, within MPMX20_00084 at 87.794 kb on + strand, within MPMX20_00084 at 87.816 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.837 kb on - strand at 87.837 kb on - strand at 88.062 kb on - strand at 88.152 kb on - strand, within MPMX20_00085 at 88.256 kb on - strand, within MPMX20_00085 at 88.284 kb on + strand, within MPMX20_00085 at 88.390 kb on + strand, within MPMX20_00085 at 88.448 kb on - strand, within MPMX20_00085 at 88.599 kb on + strand, within MPMX20_00085 at 88.601 kb on + strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.609 kb on - strand, within MPMX20_00085 at 88.636 kb on + strand, within MPMX20_00085 at 88.656 kb on + strand, within MPMX20_00085 at 88.656 kb on + strand, within MPMX20_00085 at 88.687 kb on - strand at 88.922 kb on + strand at 88.987 kb on + strand, within MPMX20_00086 at 89.012 kb on - strand, within MPMX20_00086
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196 remove 84,981 + MPMX20_00083 0.19 -1.3 84,981 + MPMX20_00083 0.19 +0.5 84,989 - MPMX20_00083 0.20 -1.3 84,989 - MPMX20_00083 0.20 +1.3 85,043 - MPMX20_00083 0.24 -0.8 85,043 - MPMX20_00083 0.24 -3.2 85,186 - MPMX20_00083 0.36 +0.8 85,189 + MPMX20_00083 0.36 +0.2 85,237 + MPMX20_00083 0.40 +1.2 85,237 + MPMX20_00083 0.40 -1.3 85,259 + MPMX20_00083 0.42 -1.8 85,262 - MPMX20_00083 0.42 -0.3 85,381 + MPMX20_00083 0.52 +0.2 85,381 + MPMX20_00083 0.52 +0.8 85,439 - MPMX20_00083 0.57 -1.1 85,486 + MPMX20_00083 0.61 +1.9 85,490 + MPMX20_00083 0.61 -1.4 85,624 + MPMX20_00083 0.72 +0.2 85,629 + MPMX20_00083 0.73 -0.7 85,656 - MPMX20_00083 0.75 -0.3 85,696 + MPMX20_00083 0.78 -2.3 85,713 - MPMX20_00083 0.80 -1.9 85,787 + MPMX20_00083 0.86 -2.3 85,879 - +0.3 85,931 - -0.8 86,024 - -0.1 86,024 - +1.2 86,024 - -2.6 86,316 + MPMX20_00084 0.17 -0.9 86,372 - MPMX20_00084 0.20 +0.0 86,461 - MPMX20_00084 0.24 +1.2 86,477 + MPMX20_00084 0.25 +0.7 86,525 + MPMX20_00084 0.27 -0.5 86,547 + MPMX20_00084 0.28 -1.0 86,555 - MPMX20_00084 0.29 -1.6 86,584 - MPMX20_00084 0.30 +2.5 86,584 - MPMX20_00084 0.30 -2.0 86,654 - MPMX20_00084 0.33 -1.0 86,670 + MPMX20_00084 0.34 -0.3 86,738 - MPMX20_00084 0.38 -2.9 86,753 - MPMX20_00084 0.38 -0.8 86,798 - MPMX20_00084 0.40 +0.7 86,831 - MPMX20_00084 0.42 -0.1 86,839 + MPMX20_00084 0.42 -1.2 86,880 + MPMX20_00084 0.44 -0.7 86,949 + MPMX20_00084 0.48 +0.5 86,962 + MPMX20_00084 0.48 -0.3 86,987 + MPMX20_00084 0.49 -2.7 86,987 + MPMX20_00084 0.49 -1.7 86,997 + MPMX20_00084 0.50 -1.8 87,020 - MPMX20_00084 0.51 -1.2 87,037 + MPMX20_00084 0.52 +0.9 87,040 + MPMX20_00084 0.52 -0.2 87,101 - MPMX20_00084 0.55 -1.1 87,151 + MPMX20_00084 0.57 +0.9 87,219 + MPMX20_00084 0.61 -0.1 87,284 + MPMX20_00084 0.64 -1.4 87,292 - MPMX20_00084 0.64 -1.1 87,339 + MPMX20_00084 0.66 -1.0 87,363 + MPMX20_00084 0.68 -1.6 87,371 - MPMX20_00084 0.68 -0.7 87,404 + MPMX20_00084 0.70 -2.3 87,411 + MPMX20_00084 0.70 -1.5 87,441 - MPMX20_00084 0.71 -0.2 87,441 - MPMX20_00084 0.71 +1.3 87,457 - MPMX20_00084 0.72 -0.1 87,463 + MPMX20_00084 0.72 -1.0 87,463 + MPMX20_00084 0.72 -2.5 87,464 + MPMX20_00084 0.72 -2.8 87,493 - MPMX20_00084 0.74 -2.1 87,511 + MPMX20_00084 0.75 -1.2 87,552 + MPMX20_00084 0.77 -2.6 87,609 + MPMX20_00084 0.79 -1.6 87,617 - MPMX20_00084 0.80 -1.0 87,627 - MPMX20_00084 0.80 -0.7 87,689 + MPMX20_00084 0.83 +0.5 87,694 + MPMX20_00084 0.83 -1.4 87,694 + MPMX20_00084 0.83 -0.5 87,694 + MPMX20_00084 0.83 -1.1 87,697 - MPMX20_00084 0.84 +2.5 87,697 - MPMX20_00084 0.84 -1.6 87,702 + MPMX20_00084 0.84 +3.5 87,702 - MPMX20_00084 0.84 -0.7 87,702 - MPMX20_00084 0.84 -2.2 87,792 - MPMX20_00084 0.88 -0.3 87,794 + MPMX20_00084 0.88 +0.2 87,816 + MPMX20_00084 0.89 -1.2 87,829 + MPMX20_00084 0.90 -3.1 87,829 + MPMX20_00084 0.90 -1.5 87,829 + MPMX20_00084 0.90 -1.5 87,837 - -2.7 87,837 - -3.1 88,062 - -0.6 88,152 - MPMX20_00085 0.16 -3.0 88,256 - MPMX20_00085 0.31 +1.5 88,284 + MPMX20_00085 0.35 -2.0 88,390 + MPMX20_00085 0.50 -0.1 88,448 - MPMX20_00085 0.59 -0.5 88,599 + MPMX20_00085 0.81 +2.5 88,601 + MPMX20_00085 0.81 +1.4 88,607 - MPMX20_00085 0.82 -0.5 88,607 - MPMX20_00085 0.82 -1.3 88,607 - MPMX20_00085 0.82 +0.6 88,607 - MPMX20_00085 0.82 +1.4 88,607 - MPMX20_00085 0.82 +0.3 88,609 - MPMX20_00085 0.82 -1.4 88,636 + MPMX20_00085 0.86 -1.3 88,656 + MPMX20_00085 0.89 -1.7 88,656 + MPMX20_00085 0.89 +0.2 88,687 - -0.1 88,922 + -1.1 88,987 + MPMX20_00086 0.12 -0.9 89,012 - MPMX20_00086 0.13 -2.0
Or see this region's nucleotide sequence