Experiment: 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00001 and MPMX20_00002 are separated by 4 nucleotides MPMX20_00002 and MPMX20_00003 are separated by 147 nucleotides
MPMX20_00001: MPMX20_00001 - Chromosomal replication initiator protein DnaA, at 70 to 1,398
_00001
MPMX20_00002: MPMX20_00002 - Beta sliding clamp, at 1,403 to 2,503
_00002
MPMX20_00003: MPMX20_00003 - DNA replication and repair protein RecF, at 2,651 to 3,724
_00003
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2.508 kb on - strand at 2.508 kb on - strand at 2.510 kb on + strand at 2.533 kb on + strand at 2.568 kb on - strand at 2.775 kb on - strand, within MPMX20_00003 at 2.813 kb on + strand, within MPMX20_00003 at 2.850 kb on + strand, within MPMX20_00003 at 2.850 kb on + strand, within MPMX20_00003 at 2.858 kb on - strand, within MPMX20_00003 at 2.873 kb on - strand, within MPMX20_00003 at 2.905 kb on + strand, within MPMX20_00003 at 2.913 kb on - strand, within MPMX20_00003 at 2.936 kb on + strand, within MPMX20_00003 at 2.980 kb on + strand, within MPMX20_00003 at 2.988 kb on - strand, within MPMX20_00003 at 2.988 kb on - strand, within MPMX20_00003 at 3.012 kb on + strand, within MPMX20_00003 at 3.020 kb on - strand, within MPMX20_00003 at 3.097 kb on + strand, within MPMX20_00003 at 3.098 kb on + strand, within MPMX20_00003 at 3.098 kb on + strand, within MPMX20_00003 at 3.105 kb on - strand, within MPMX20_00003 at 3.111 kb on - strand, within MPMX20_00003 at 3.128 kb on - strand, within MPMX20_00003 at 3.130 kb on + strand, within MPMX20_00003 at 3.135 kb on + strand, within MPMX20_00003 at 3.138 kb on - strand, within MPMX20_00003 at 3.138 kb on - strand, within MPMX20_00003 at 3.138 kb on - strand, within MPMX20_00003 at 3.138 kb on - strand, within MPMX20_00003 at 3.143 kb on - strand, within MPMX20_00003 at 3.143 kb on - strand, within MPMX20_00003 at 3.145 kb on + strand, within MPMX20_00003 at 3.160 kb on - strand, within MPMX20_00003 at 3.164 kb on + strand, within MPMX20_00003 at 3.164 kb on + strand, within MPMX20_00003 at 3.227 kb on + strand, within MPMX20_00003 at 3.260 kb on + strand, within MPMX20_00003 at 3.275 kb on - strand, within MPMX20_00003 at 3.277 kb on + strand, within MPMX20_00003 at 3.277 kb on + strand, within MPMX20_00003 at 3.285 kb on - strand, within MPMX20_00003 at 3.298 kb on + strand, within MPMX20_00003 at 3.395 kb on - strand, within MPMX20_00003 at 3.459 kb on + strand, within MPMX20_00003 at 3.476 kb on + strand, within MPMX20_00003 at 3.477 kb on - strand, within MPMX20_00003 at 3.484 kb on - strand, within MPMX20_00003
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, 1:1 coculture with Agromyces sp. SAI_196 remove 2,508 - -1.2 2,508 - -0.1 2,510 + -1.2 2,533 + -0.0 2,568 - +4.5 2,775 - MPMX20_00003 0.12 +3.2 2,813 + MPMX20_00003 0.15 -2.8 2,850 + MPMX20_00003 0.19 -0.3 2,850 + MPMX20_00003 0.19 -0.7 2,858 - MPMX20_00003 0.19 -2.4 2,873 - MPMX20_00003 0.21 -1.1 2,905 + MPMX20_00003 0.24 -1.1 2,913 - MPMX20_00003 0.24 -1.5 2,936 + MPMX20_00003 0.27 -2.4 2,980 + MPMX20_00003 0.31 -0.8 2,988 - MPMX20_00003 0.31 +4.5 2,988 - MPMX20_00003 0.31 +0.1 3,012 + MPMX20_00003 0.34 -1.1 3,020 - MPMX20_00003 0.34 -0.3 3,097 + MPMX20_00003 0.42 -0.1 3,098 + MPMX20_00003 0.42 +0.5 3,098 + MPMX20_00003 0.42 +0.2 3,105 - MPMX20_00003 0.42 +0.9 3,111 - MPMX20_00003 0.43 +2.0 3,128 - MPMX20_00003 0.44 -0.1 3,130 + MPMX20_00003 0.45 +1.5 3,135 + MPMX20_00003 0.45 +1.0 3,138 - MPMX20_00003 0.45 +1.5 3,138 - MPMX20_00003 0.45 -0.7 3,138 - MPMX20_00003 0.45 -0.5 3,138 - MPMX20_00003 0.45 -1.3 3,143 - MPMX20_00003 0.46 -1.4 3,143 - MPMX20_00003 0.46 +1.0 3,145 + MPMX20_00003 0.46 +1.3 3,160 - MPMX20_00003 0.47 -1.7 3,164 + MPMX20_00003 0.48 -0.5 3,164 + MPMX20_00003 0.48 -1.2 3,227 + MPMX20_00003 0.54 +1.5 3,260 + MPMX20_00003 0.57 -0.8 3,275 - MPMX20_00003 0.58 +0.2 3,277 + MPMX20_00003 0.58 -0.8 3,277 + MPMX20_00003 0.58 -0.1 3,285 - MPMX20_00003 0.59 -0.5 3,298 + MPMX20_00003 0.60 -1.3 3,395 - MPMX20_00003 0.69 -3.1 3,459 + MPMX20_00003 0.75 -1.4 3,476 + MPMX20_00003 0.77 +1.3 3,477 - MPMX20_00003 0.77 -1.6 3,484 - MPMX20_00003 0.78 +1.4
Or see this region's nucleotide sequence