Experiment: M9 plate, 1:1 coculture with Chryseobacterium proteolyticum TBS_027
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04006 and MPMX20_04007 are separated by 29 nucleotides MPMX20_04007 and MPMX20_04008 are separated by 1 nucleotides MPMX20_04008 and MPMX20_04009 overlap by 4 nucleotides MPMX20_04009 and MPMX20_04010 are separated by 209 nucleotides MPMX20_04010 and MPMX20_04011 are separated by 72 nucleotides
MPMX20_04006: MPMX20_04006 - Protein YqjC, at 4,204,703 to 4,205,071
_04006
MPMX20_04007: MPMX20_04007 - putative protein YqjD, at 4,205,101 to 4,205,406
_04007
MPMX20_04008: MPMX20_04008 - Inner membrane protein YqjE, at 4,205,408 to 4,205,806
_04008
MPMX20_04009: MPMX20_04009 - hypothetical protein, at 4,205,803 to 4,206,099
_04009
MPMX20_04010: MPMX20_04010 - Inner membrane protein YqjF, at 4,206,309 to 4,206,701
_04010
MPMX20_04011: MPMX20_04011 - Glutathionyl-hydroquinone reductase YqjG, at 4,206,774 to 4,207,760
_04011
Position (kb) 4205
4206
4207 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4204.839 kb on + strand, within MPMX20_04006 at 4204.847 kb on - strand, within MPMX20_04006 at 4204.847 kb on - strand, within MPMX20_04006 at 4204.847 kb on - strand, within MPMX20_04006 at 4204.847 kb on - strand, within MPMX20_04006 at 4204.884 kb on + strand, within MPMX20_04006 at 4204.897 kb on + strand, within MPMX20_04006 at 4205.020 kb on + strand, within MPMX20_04006 at 4205.110 kb on + strand at 4205.167 kb on - strand, within MPMX20_04007 at 4205.167 kb on - strand, within MPMX20_04007 at 4205.228 kb on - strand, within MPMX20_04007 at 4205.228 kb on - strand, within MPMX20_04007 at 4205.252 kb on + strand, within MPMX20_04007 at 4205.252 kb on + strand, within MPMX20_04007 at 4205.252 kb on + strand, within MPMX20_04007 at 4205.287 kb on + strand, within MPMX20_04007 at 4205.355 kb on + strand, within MPMX20_04007 at 4205.355 kb on - strand, within MPMX20_04007 at 4205.361 kb on - strand, within MPMX20_04007 at 4205.380 kb on - strand at 4205.380 kb on - strand at 4205.482 kb on + strand, within MPMX20_04008 at 4205.531 kb on - strand, within MPMX20_04008 at 4205.565 kb on + strand, within MPMX20_04008 at 4205.576 kb on + strand, within MPMX20_04008 at 4205.615 kb on + strand, within MPMX20_04008 at 4205.762 kb on + strand, within MPMX20_04008 at 4205.770 kb on - strand at 4205.793 kb on - strand at 4205.793 kb on - strand at 4205.816 kb on - strand at 4205.905 kb on - strand, within MPMX20_04009 at 4205.927 kb on + strand, within MPMX20_04009 at 4205.939 kb on + strand, within MPMX20_04009 at 4205.954 kb on + strand, within MPMX20_04009 at 4205.954 kb on + strand, within MPMX20_04009 at 4205.973 kb on - strand, within MPMX20_04009 at 4205.975 kb on + strand, within MPMX20_04009 at 4206.129 kb on - strand at 4206.443 kb on - strand, within MPMX20_04010 at 4206.522 kb on + strand, within MPMX20_04010 at 4206.527 kb on + strand, within MPMX20_04010 at 4206.537 kb on + strand, within MPMX20_04010 at 4206.541 kb on + strand, within MPMX20_04010 at 4206.545 kb on - strand, within MPMX20_04010 at 4206.585 kb on + strand, within MPMX20_04010 at 4206.593 kb on - strand, within MPMX20_04010 at 4206.631 kb on + strand, within MPMX20_04010 at 4206.644 kb on + strand, within MPMX20_04010 at 4206.654 kb on + strand, within MPMX20_04010 at 4206.655 kb on + strand, within MPMX20_04010 at 4206.704 kb on + strand at 4206.717 kb on - strand at 4206.964 kb on - strand, within MPMX20_04011 at 4206.967 kb on - strand, within MPMX20_04011 at 4207.002 kb on - strand, within MPMX20_04011 at 4207.031 kb on + strand, within MPMX20_04011
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Chryseobacterium proteolyticum TBS_027 remove 4,204,839 + MPMX20_04006 0.37 +0.7 4,204,847 - MPMX20_04006 0.39 -0.9 4,204,847 - MPMX20_04006 0.39 -1.5 4,204,847 - MPMX20_04006 0.39 -0.5 4,204,847 - MPMX20_04006 0.39 -0.4 4,204,884 + MPMX20_04006 0.49 -0.5 4,204,897 + MPMX20_04006 0.53 -0.7 4,205,020 + MPMX20_04006 0.86 -1.5 4,205,110 + -0.3 4,205,167 - MPMX20_04007 0.22 +1.4 4,205,167 - MPMX20_04007 0.22 -0.7 4,205,228 - MPMX20_04007 0.42 -1.9 4,205,228 - MPMX20_04007 0.42 -1.2 4,205,252 + MPMX20_04007 0.49 -1.3 4,205,252 + MPMX20_04007 0.49 +0.7 4,205,252 + MPMX20_04007 0.49 -0.1 4,205,287 + MPMX20_04007 0.61 +0.7 4,205,355 + MPMX20_04007 0.83 +1.8 4,205,355 - MPMX20_04007 0.83 -0.4 4,205,361 - MPMX20_04007 0.85 +0.2 4,205,380 - -1.5 4,205,380 - +0.1 4,205,482 + MPMX20_04008 0.19 -0.6 4,205,531 - MPMX20_04008 0.31 -0.3 4,205,565 + MPMX20_04008 0.39 -2.0 4,205,576 + MPMX20_04008 0.42 +0.8 4,205,615 + MPMX20_04008 0.52 +0.4 4,205,762 + MPMX20_04008 0.89 +0.1 4,205,770 - -1.1 4,205,793 - +0.4 4,205,793 - -0.6 4,205,816 - +0.8 4,205,905 - MPMX20_04009 0.34 +0.6 4,205,927 + MPMX20_04009 0.42 +2.4 4,205,939 + MPMX20_04009 0.46 -0.4 4,205,954 + MPMX20_04009 0.51 +0.9 4,205,954 + MPMX20_04009 0.51 +1.2 4,205,973 - MPMX20_04009 0.57 +0.0 4,205,975 + MPMX20_04009 0.58 +0.2 4,206,129 - +0.4 4,206,443 - MPMX20_04010 0.34 +0.7 4,206,522 + MPMX20_04010 0.54 -0.8 4,206,527 + MPMX20_04010 0.55 -1.5 4,206,537 + MPMX20_04010 0.58 +1.2 4,206,541 + MPMX20_04010 0.59 -1.1 4,206,545 - MPMX20_04010 0.60 -0.9 4,206,585 + MPMX20_04010 0.70 +1.4 4,206,593 - MPMX20_04010 0.72 -2.0 4,206,631 + MPMX20_04010 0.82 +0.0 4,206,644 + MPMX20_04010 0.85 +0.4 4,206,654 + MPMX20_04010 0.88 -1.0 4,206,655 + MPMX20_04010 0.88 -0.2 4,206,704 + +0.7 4,206,717 - -0.7 4,206,964 - MPMX20_04011 0.19 -1.5 4,206,967 - MPMX20_04011 0.20 -1.7 4,207,002 - MPMX20_04011 0.23 -0.7 4,207,031 + MPMX20_04011 0.26 -0.6
Or see this region's nucleotide sequence