Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04108 and MPMX20_04109 are separated by 22 nucleotides MPMX20_04109 and MPMX20_04110 are separated by 82 nucleotides MPMX20_04110 and MPMX20_04111 are separated by 45 nucleotides MPMX20_04111 and MPMX20_04112 overlap by 4 nucleotides
MPMX20_04108: MPMX20_04108 - RNA polymerase sigma-54 factor, at 4,300,853 to 4,302,286
_04108
MPMX20_04109: MPMX20_04109 - Ribosome hibernation promoting factor, at 4,302,309 to 4,302,596
_04109
MPMX20_04110: MPMX20_04110 - Nitrogen regulatory protein, at 4,302,679 to 4,303,170
_04110
MPMX20_04111: MPMX20_04111 - RNase adapter protein RapZ, at 4,303,216 to 4,304,070
_04111
MPMX20_04112: MPMX20_04112 - Phosphocarrier protein NPr, at 4,304,067 to 4,304,339
_04112
Position (kb) 4302
4303
4304 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4302.180 kb on + strand at 4302.299 kb on + strand at 4302.314 kb on + strand at 4302.414 kb on - strand, within MPMX20_04109 at 4302.414 kb on - strand, within MPMX20_04109 at 4302.416 kb on + strand, within MPMX20_04109 at 4302.416 kb on + strand, within MPMX20_04109 at 4302.424 kb on - strand, within MPMX20_04109 at 4302.424 kb on - strand, within MPMX20_04109 at 4302.424 kb on - strand, within MPMX20_04109 at 4302.452 kb on + strand, within MPMX20_04109 at 4302.497 kb on - strand, within MPMX20_04109 at 4302.515 kb on + strand, within MPMX20_04109 at 4302.515 kb on + strand, within MPMX20_04109 at 4302.515 kb on + strand, within MPMX20_04109 at 4302.523 kb on - strand, within MPMX20_04109 at 4302.525 kb on + strand, within MPMX20_04109 at 4302.525 kb on + strand, within MPMX20_04109 at 4302.547 kb on + strand, within MPMX20_04109 at 4302.548 kb on + strand, within MPMX20_04109 at 4302.616 kb on - strand at 4302.657 kb on - strand at 4302.659 kb on - strand at 4302.684 kb on - strand at 4302.706 kb on - strand at 4302.740 kb on + strand, within MPMX20_04110 at 4302.740 kb on + strand, within MPMX20_04110 at 4302.838 kb on + strand, within MPMX20_04110 at 4302.881 kb on - strand, within MPMX20_04110 at 4302.934 kb on + strand, within MPMX20_04110 at 4302.953 kb on - strand, within MPMX20_04110 at 4302.955 kb on + strand, within MPMX20_04110 at 4303.014 kb on - strand, within MPMX20_04110 at 4303.026 kb on + strand, within MPMX20_04110 at 4303.105 kb on + strand, within MPMX20_04110 at 4303.105 kb on + strand, within MPMX20_04110 at 4303.105 kb on + strand, within MPMX20_04110 at 4303.105 kb on + strand, within MPMX20_04110 at 4303.113 kb on - strand, within MPMX20_04110 at 4303.113 kb on - strand, within MPMX20_04110 at 4303.113 kb on - strand, within MPMX20_04110 at 4303.114 kb on + strand, within MPMX20_04110 at 4303.196 kb on + strand at 4303.267 kb on - strand at 4303.316 kb on - strand, within MPMX20_04111 at 4303.459 kb on - strand, within MPMX20_04111 at 4303.486 kb on + strand, within MPMX20_04111 at 4303.486 kb on + strand, within MPMX20_04111 at 4303.494 kb on - strand, within MPMX20_04111 at 4303.726 kb on - strand, within MPMX20_04111 at 4303.818 kb on + strand, within MPMX20_04111 at 4303.889 kb on + strand, within MPMX20_04111 at 4303.897 kb on - strand, within MPMX20_04111 at 4303.897 kb on - strand, within MPMX20_04111 at 4304.095 kb on + strand, within MPMX20_04112 at 4304.166 kb on + strand, within MPMX20_04112
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 4,302,180 + +2.9 4,302,299 + -1.4 4,302,314 + -0.0 4,302,414 - MPMX20_04109 0.36 +1.3 4,302,414 - MPMX20_04109 0.36 -0.1 4,302,416 + MPMX20_04109 0.37 +0.3 4,302,416 + MPMX20_04109 0.37 +0.6 4,302,424 - MPMX20_04109 0.40 +0.6 4,302,424 - MPMX20_04109 0.40 +0.7 4,302,424 - MPMX20_04109 0.40 +1.0 4,302,452 + MPMX20_04109 0.50 -1.6 4,302,497 - MPMX20_04109 0.65 +0.1 4,302,515 + MPMX20_04109 0.72 +0.6 4,302,515 + MPMX20_04109 0.72 +0.3 4,302,515 + MPMX20_04109 0.72 +1.5 4,302,523 - MPMX20_04109 0.74 +0.1 4,302,525 + MPMX20_04109 0.75 -0.8 4,302,525 + MPMX20_04109 0.75 +0.4 4,302,547 + MPMX20_04109 0.83 -0.6 4,302,548 + MPMX20_04109 0.83 -1.2 4,302,616 - -1.5 4,302,657 - +2.3 4,302,659 - +0.1 4,302,684 - +1.3 4,302,706 - -0.4 4,302,740 + MPMX20_04110 0.12 -0.1 4,302,740 + MPMX20_04110 0.12 -0.6 4,302,838 + MPMX20_04110 0.32 -0.6 4,302,881 - MPMX20_04110 0.41 -0.1 4,302,934 + MPMX20_04110 0.52 +0.3 4,302,953 - MPMX20_04110 0.56 +0.1 4,302,955 + MPMX20_04110 0.56 -0.3 4,303,014 - MPMX20_04110 0.68 -0.6 4,303,026 + MPMX20_04110 0.71 +1.9 4,303,105 + MPMX20_04110 0.87 -1.6 4,303,105 + MPMX20_04110 0.87 -1.0 4,303,105 + MPMX20_04110 0.87 -1.3 4,303,105 + MPMX20_04110 0.87 -0.1 4,303,113 - MPMX20_04110 0.88 +1.7 4,303,113 - MPMX20_04110 0.88 +0.1 4,303,113 - MPMX20_04110 0.88 -0.3 4,303,114 + MPMX20_04110 0.88 -1.6 4,303,196 + -0.7 4,303,267 - +1.9 4,303,316 - MPMX20_04111 0.12 +0.7 4,303,459 - MPMX20_04111 0.28 -0.1 4,303,486 + MPMX20_04111 0.32 +0.9 4,303,486 + MPMX20_04111 0.32 -0.1 4,303,494 - MPMX20_04111 0.33 +1.0 4,303,726 - MPMX20_04111 0.60 +0.4 4,303,818 + MPMX20_04111 0.70 +0.2 4,303,889 + MPMX20_04111 0.79 -2.2 4,303,897 - MPMX20_04111 0.80 +0.6 4,303,897 - MPMX20_04111 0.80 -0.1 4,304,095 + MPMX20_04112 0.10 -0.1 4,304,166 + MPMX20_04112 0.36 -0.2
Or see this region's nucleotide sequence