Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02147 and MPMX20_02148 are separated by 2 nucleotides MPMX20_02148 and MPMX20_02149 are separated by 1 nucleotides MPMX20_02149 and MPMX20_02150 overlap by 11 nucleotides MPMX20_02150 and MPMX20_02151 overlap by 1 nucleotides
MPMX20_02147: MPMX20_02147 - 2,3-dehydroadipyl-CoA hydratase, at 2,239,453 to 2,240,220
_02147
MPMX20_02148: MPMX20_02148 - 1,2-epoxyphenylacetyl-CoA isomerase, at 2,240,223 to 2,241,008
_02148
MPMX20_02149: MPMX20_02149 - 3-hydroxyadipyl-CoA dehydrogenase, at 2,241,010 to 2,242,437
_02149
MPMX20_02150: MPMX20_02150 - Acyl-coenzyme A thioesterase PaaI, at 2,242,427 to 2,242,849
_02150
MPMX20_02151: MPMX20_02151 - 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase, at 2,242,849 to 2,244,054
_02151
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2240.037 kb on - strand, within MPMX20_02147 at 2240.048 kb on - strand, within MPMX20_02147 at 2240.116 kb on + strand, within MPMX20_02147 at 2240.116 kb on + strand, within MPMX20_02147 at 2240.116 kb on + strand, within MPMX20_02147 at 2240.124 kb on - strand, within MPMX20_02147 at 2240.130 kb on + strand, within MPMX20_02147 at 2240.142 kb on + strand, within MPMX20_02147 at 2240.160 kb on - strand at 2240.271 kb on - strand at 2240.276 kb on - strand at 2240.359 kb on - strand, within MPMX20_02148 at 2240.563 kb on - strand, within MPMX20_02148 at 2240.568 kb on - strand, within MPMX20_02148 at 2240.596 kb on - strand, within MPMX20_02148 at 2240.622 kb on + strand, within MPMX20_02148 at 2240.664 kb on - strand, within MPMX20_02148 at 2240.706 kb on - strand, within MPMX20_02148 at 2240.711 kb on - strand, within MPMX20_02148 at 2240.741 kb on + strand, within MPMX20_02148 at 2240.761 kb on + strand, within MPMX20_02148 at 2240.768 kb on + strand, within MPMX20_02148 at 2240.812 kb on - strand, within MPMX20_02148 at 2240.836 kb on + strand, within MPMX20_02148 at 2240.839 kb on - strand, within MPMX20_02148 at 2240.844 kb on - strand, within MPMX20_02148 at 2240.844 kb on - strand, within MPMX20_02148 at 2240.844 kb on - strand, within MPMX20_02148 at 2240.887 kb on - strand, within MPMX20_02148 at 2240.960 kb on + strand at 2240.976 kb on + strand at 2241.351 kb on - strand, within MPMX20_02149 at 2241.351 kb on - strand, within MPMX20_02149 at 2241.565 kb on + strand, within MPMX20_02149 at 2241.566 kb on + strand, within MPMX20_02149 at 2241.574 kb on - strand, within MPMX20_02149 at 2241.605 kb on + strand, within MPMX20_02149 at 2241.631 kb on - strand, within MPMX20_02149 at 2241.706 kb on - strand, within MPMX20_02149 at 2241.754 kb on - strand, within MPMX20_02149 at 2241.869 kb on + strand, within MPMX20_02149 at 2241.919 kb on - strand, within MPMX20_02149 at 2242.087 kb on - strand, within MPMX20_02149 at 2242.257 kb on - strand, within MPMX20_02149 at 2242.307 kb on - strand at 2242.367 kb on - strand at 2242.383 kb on + strand at 2242.388 kb on + strand at 2242.419 kb on + strand at 2242.419 kb on - strand at 2242.419 kb on - strand at 2242.453 kb on - strand at 2242.634 kb on + strand, within MPMX20_02150 at 2242.635 kb on + strand, within MPMX20_02150 at 2242.655 kb on + strand, within MPMX20_02150 at 2242.723 kb on + strand, within MPMX20_02150 at 2242.731 kb on - strand, within MPMX20_02150 at 2242.759 kb on - strand, within MPMX20_02150 at 2242.838 kb on + strand at 2242.914 kb on - strand at 2242.937 kb on + strand at 2242.950 kb on - strand at 2243.087 kb on - strand, within MPMX20_02151 at 2243.097 kb on - strand, within MPMX20_02151 at 2243.120 kb on + strand, within MPMX20_02151 at 2243.128 kb on - strand, within MPMX20_02151 at 2243.154 kb on + strand, within MPMX20_02151 at 2243.229 kb on + strand, within MPMX20_02151 at 2243.237 kb on - strand, within MPMX20_02151 at 2243.286 kb on - strand, within MPMX20_02151 at 2243.423 kb on + strand, within MPMX20_02151
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 2,240,037 - MPMX20_02147 0.76 -1.2 2,240,048 - MPMX20_02147 0.77 -2.2 2,240,116 + MPMX20_02147 0.86 -1.7 2,240,116 + MPMX20_02147 0.86 -2.7 2,240,116 + MPMX20_02147 0.86 -1.8 2,240,124 - MPMX20_02147 0.87 -0.8 2,240,130 + MPMX20_02147 0.88 -2.0 2,240,142 + MPMX20_02147 0.90 -2.4 2,240,160 - +0.1 2,240,271 - +1.3 2,240,276 - -1.6 2,240,359 - MPMX20_02148 0.17 -0.3 2,240,563 - MPMX20_02148 0.43 -0.1 2,240,568 - MPMX20_02148 0.44 -1.1 2,240,596 - MPMX20_02148 0.47 -0.3 2,240,622 + MPMX20_02148 0.51 -0.1 2,240,664 - MPMX20_02148 0.56 +0.0 2,240,706 - MPMX20_02148 0.61 -1.0 2,240,711 - MPMX20_02148 0.62 -0.4 2,240,741 + MPMX20_02148 0.66 -0.2 2,240,761 + MPMX20_02148 0.68 -1.0 2,240,768 + MPMX20_02148 0.69 -0.2 2,240,812 - MPMX20_02148 0.75 +1.1 2,240,836 + MPMX20_02148 0.78 -1.6 2,240,839 - MPMX20_02148 0.78 +0.6 2,240,844 - MPMX20_02148 0.79 +0.1 2,240,844 - MPMX20_02148 0.79 -1.6 2,240,844 - MPMX20_02148 0.79 -1.2 2,240,887 - MPMX20_02148 0.84 +0.5 2,240,960 + -1.9 2,240,976 + -0.9 2,241,351 - MPMX20_02149 0.24 -0.4 2,241,351 - MPMX20_02149 0.24 -0.1 2,241,565 + MPMX20_02149 0.39 +0.4 2,241,566 + MPMX20_02149 0.39 -0.3 2,241,574 - MPMX20_02149 0.39 -0.8 2,241,605 + MPMX20_02149 0.42 -1.5 2,241,631 - MPMX20_02149 0.43 -1.8 2,241,706 - MPMX20_02149 0.49 +0.9 2,241,754 - MPMX20_02149 0.52 -0.4 2,241,869 + MPMX20_02149 0.60 -0.3 2,241,919 - MPMX20_02149 0.64 -2.3 2,242,087 - MPMX20_02149 0.75 -1.8 2,242,257 - MPMX20_02149 0.87 -2.1 2,242,307 - -2.9 2,242,367 - -0.8 2,242,383 + -1.2 2,242,388 + -1.4 2,242,419 + -0.3 2,242,419 - +0.1 2,242,419 - +1.0 2,242,453 - -0.6 2,242,634 + MPMX20_02150 0.49 -1.5 2,242,635 + MPMX20_02150 0.49 -1.9 2,242,655 + MPMX20_02150 0.54 +0.5 2,242,723 + MPMX20_02150 0.70 +0.7 2,242,731 - MPMX20_02150 0.72 -0.3 2,242,759 - MPMX20_02150 0.78 +1.3 2,242,838 + +1.1 2,242,914 - -0.4 2,242,937 + -0.1 2,242,950 - +0.0 2,243,087 - MPMX20_02151 0.20 +0.9 2,243,097 - MPMX20_02151 0.21 +0.5 2,243,120 + MPMX20_02151 0.22 -1.5 2,243,128 - MPMX20_02151 0.23 -1.0 2,243,154 + MPMX20_02151 0.25 -0.3 2,243,229 + MPMX20_02151 0.32 +2.4 2,243,237 - MPMX20_02151 0.32 -0.3 2,243,286 - MPMX20_02151 0.36 +3.0 2,243,423 + MPMX20_02151 0.48 +0.4
Or see this region's nucleotide sequence