Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01662 and MPMX20_01663 are separated by 90 nucleotides MPMX20_01663 and MPMX20_01664 overlap by 4 nucleotides
MPMX20_01662: MPMX20_01662 - hypothetical protein, at 1,761,028 to 1,761,960
_01662
MPMX20_01663: MPMX20_01663 - hypothetical protein, at 1,762,051 to 1,762,437
_01663
MPMX20_01664: MPMX20_01664 - 1-carboxybiuret hydrolase subunit AtzE, at 1,762,434 to 1,763,831
_01664
Position (kb)
1762
1763 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1761.106 kb on - strand at 1761.130 kb on + strand, within MPMX20_01662 at 1761.190 kb on - strand, within MPMX20_01662 at 1761.291 kb on - strand, within MPMX20_01662 at 1761.391 kb on + strand, within MPMX20_01662 at 1761.468 kb on + strand, within MPMX20_01662 at 1761.468 kb on + strand, within MPMX20_01662 at 1761.476 kb on - strand, within MPMX20_01662 at 1761.476 kb on - strand, within MPMX20_01662 at 1761.484 kb on + strand, within MPMX20_01662 at 1761.588 kb on - strand, within MPMX20_01662 at 1761.603 kb on - strand, within MPMX20_01662 at 1761.633 kb on - strand, within MPMX20_01662 at 1761.661 kb on - strand, within MPMX20_01662 at 1761.664 kb on + strand, within MPMX20_01662 at 1761.717 kb on + strand, within MPMX20_01662 at 1761.723 kb on - strand, within MPMX20_01662 at 1761.735 kb on + strand, within MPMX20_01662 at 1761.735 kb on + strand, within MPMX20_01662 at 1761.743 kb on - strand, within MPMX20_01662 at 1761.780 kb on + strand, within MPMX20_01662 at 1761.875 kb on + strand at 1761.880 kb on + strand at 1761.900 kb on + strand at 1761.900 kb on + strand at 1761.905 kb on + strand at 1761.913 kb on - strand at 1761.963 kb on + strand at 1761.971 kb on - strand at 1761.993 kb on - strand at 1762.003 kb on - strand at 1762.039 kb on + strand at 1762.049 kb on - strand at 1762.074 kb on - strand at 1762.074 kb on - strand at 1762.172 kb on + strand, within MPMX20_01663 at 1762.172 kb on + strand, within MPMX20_01663 at 1762.180 kb on - strand, within MPMX20_01663 at 1762.180 kb on - strand, within MPMX20_01663 at 1762.180 kb on - strand, within MPMX20_01663 at 1762.180 kb on - strand, within MPMX20_01663 at 1762.181 kb on + strand, within MPMX20_01663 at 1762.271 kb on + strand, within MPMX20_01663 at 1762.286 kb on - strand, within MPMX20_01663 at 1762.298 kb on - strand, within MPMX20_01663 at 1762.316 kb on + strand, within MPMX20_01663 at 1762.324 kb on - strand, within MPMX20_01663 at 1762.346 kb on - strand, within MPMX20_01663 at 1762.357 kb on - strand, within MPMX20_01663 at 1762.375 kb on + strand, within MPMX20_01663 at 1762.381 kb on - strand, within MPMX20_01663 at 1762.388 kb on - strand, within MPMX20_01663 at 1762.421 kb on - strand at 1762.579 kb on - strand, within MPMX20_01664 at 1762.623 kb on + strand, within MPMX20_01664 at 1762.640 kb on - strand, within MPMX20_01664 at 1762.818 kb on - strand, within MPMX20_01664 at 1762.863 kb on - strand, within MPMX20_01664 at 1762.929 kb on - strand, within MPMX20_01664 at 1762.973 kb on - strand, within MPMX20_01664 at 1762.973 kb on - strand, within MPMX20_01664 at 1763.085 kb on + strand, within MPMX20_01664 at 1763.093 kb on - strand, within MPMX20_01664 at 1763.141 kb on - strand, within MPMX20_01664 at 1763.203 kb on - strand, within MPMX20_01664 at 1763.258 kb on - strand, within MPMX20_01664 at 1763.331 kb on - strand, within MPMX20_01664 at 1763.407 kb on - strand, within MPMX20_01664
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 1,761,106 - -0.6 1,761,130 + MPMX20_01662 0.11 -0.1 1,761,190 - MPMX20_01662 0.17 +0.2 1,761,291 - MPMX20_01662 0.28 -0.4 1,761,391 + MPMX20_01662 0.39 -0.4 1,761,468 + MPMX20_01662 0.47 -0.7 1,761,468 + MPMX20_01662 0.47 -0.2 1,761,476 - MPMX20_01662 0.48 -0.5 1,761,476 - MPMX20_01662 0.48 +0.6 1,761,484 + MPMX20_01662 0.49 -1.7 1,761,588 - MPMX20_01662 0.60 +0.5 1,761,603 - MPMX20_01662 0.62 +1.7 1,761,633 - MPMX20_01662 0.65 -0.4 1,761,661 - MPMX20_01662 0.68 -0.7 1,761,664 + MPMX20_01662 0.68 +0.1 1,761,717 + MPMX20_01662 0.74 +0.1 1,761,723 - MPMX20_01662 0.74 -1.9 1,761,735 + MPMX20_01662 0.76 -1.2 1,761,735 + MPMX20_01662 0.76 -0.6 1,761,743 - MPMX20_01662 0.77 -0.6 1,761,780 + MPMX20_01662 0.81 -1.3 1,761,875 + +0.7 1,761,880 + +0.3 1,761,900 + -0.1 1,761,900 + +0.6 1,761,905 + +1.5 1,761,913 - +1.1 1,761,963 + -0.3 1,761,971 - -1.0 1,761,993 - -1.1 1,762,003 - -2.2 1,762,039 + +0.4 1,762,049 - -1.8 1,762,074 - -0.0 1,762,074 - +0.1 1,762,172 + MPMX20_01663 0.31 -1.5 1,762,172 + MPMX20_01663 0.31 -0.9 1,762,180 - MPMX20_01663 0.33 -1.4 1,762,180 - MPMX20_01663 0.33 +1.7 1,762,180 - MPMX20_01663 0.33 +2.7 1,762,180 - MPMX20_01663 0.33 -0.6 1,762,181 + MPMX20_01663 0.34 +0.7 1,762,271 + MPMX20_01663 0.57 +1.0 1,762,286 - MPMX20_01663 0.61 +0.0 1,762,298 - MPMX20_01663 0.64 -0.5 1,762,316 + MPMX20_01663 0.68 +1.7 1,762,324 - MPMX20_01663 0.71 -0.2 1,762,346 - MPMX20_01663 0.76 -0.1 1,762,357 - MPMX20_01663 0.79 -0.3 1,762,375 + MPMX20_01663 0.84 -0.5 1,762,381 - MPMX20_01663 0.85 +1.0 1,762,388 - MPMX20_01663 0.87 -0.6 1,762,421 - -0.2 1,762,579 - MPMX20_01664 0.10 -1.9 1,762,623 + MPMX20_01664 0.14 +0.3 1,762,640 - MPMX20_01664 0.15 -1.7 1,762,818 - MPMX20_01664 0.27 -1.4 1,762,863 - MPMX20_01664 0.31 +0.4 1,762,929 - MPMX20_01664 0.35 -1.3 1,762,973 - MPMX20_01664 0.39 +0.7 1,762,973 - MPMX20_01664 0.39 -0.7 1,763,085 + MPMX20_01664 0.47 +0.9 1,763,093 - MPMX20_01664 0.47 +0.1 1,763,141 - MPMX20_01664 0.51 +0.6 1,763,203 - MPMX20_01664 0.55 +0.1 1,763,258 - MPMX20_01664 0.59 +0.5 1,763,331 - MPMX20_01664 0.64 -2.2 1,763,407 - MPMX20_01664 0.70 +2.0
Or see this region's nucleotide sequence