Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01549 and MPMX20_01550 are separated by 114 nucleotides MPMX20_01550 and MPMX20_01551 are separated by 517 nucleotides
MPMX20_01549: MPMX20_01549 - ATP-binding/permease protein CydD, at 1,621,491 to 1,623,257
_01549
MPMX20_01550: MPMX20_01550 - Thioredoxin reductase, at 1,623,372 to 1,624,367
_01550
MPMX20_01551: MPMX20_01551 - Leucine-responsive regulatory protein, at 1,624,885 to 1,625,379
_01551
Position (kb)
1623
1624
1625 Strain fitness (log2 ratio)
-1
0
1
2 at 1622.504 kb on + strand, within MPMX20_01549 at 1622.534 kb on + strand, within MPMX20_01549 at 1622.534 kb on - strand, within MPMX20_01549 at 1622.540 kb on - strand, within MPMX20_01549 at 1622.881 kb on - strand, within MPMX20_01549 at 1622.913 kb on - strand, within MPMX20_01549 at 1623.053 kb on + strand, within MPMX20_01549 at 1623.150 kb on - strand at 1623.223 kb on - strand at 1623.228 kb on + strand at 1623.578 kb on - strand, within MPMX20_01550 at 1623.670 kb on + strand, within MPMX20_01550 at 1623.791 kb on - strand, within MPMX20_01550 at 1623.835 kb on - strand, within MPMX20_01550 at 1624.267 kb on + strand, within MPMX20_01550 at 1624.267 kb on + strand, within MPMX20_01550 at 1624.267 kb on + strand, within MPMX20_01550 at 1624.275 kb on - strand at 1624.275 kb on - strand at 1624.275 kb on - strand at 1624.323 kb on + strand at 1624.323 kb on + strand at 1624.323 kb on + strand at 1624.325 kb on + strand at 1624.333 kb on - strand at 1624.336 kb on + strand at 1624.343 kb on - strand at 1624.454 kb on - strand at 1624.467 kb on + strand at 1624.524 kb on - strand at 1624.526 kb on - strand at 1624.583 kb on + strand at 1624.647 kb on - strand at 1624.695 kb on + strand at 1624.716 kb on - strand at 1624.819 kb on + strand at 1624.819 kb on + strand at 1624.819 kb on + strand at 1624.827 kb on - strand at 1624.827 kb on - strand at 1624.831 kb on + strand at 1624.832 kb on - strand at 1624.839 kb on - strand at 1624.841 kb on + strand at 1624.841 kb on + strand at 1624.841 kb on + strand at 1624.842 kb on + strand at 1624.842 kb on + strand at 1624.843 kb on + strand at 1624.851 kb on - strand at 1624.855 kb on - strand at 1624.856 kb on - strand at 1624.863 kb on + strand at 1624.923 kb on - strand at 1624.923 kb on - strand at 1625.019 kb on + strand, within MPMX20_01551 at 1625.027 kb on - strand, within MPMX20_01551 at 1625.034 kb on - strand, within MPMX20_01551 at 1625.061 kb on + strand, within MPMX20_01551 at 1625.061 kb on + strand, within MPMX20_01551 at 1625.061 kb on + strand, within MPMX20_01551 at 1625.069 kb on - strand, within MPMX20_01551 at 1625.069 kb on - strand, within MPMX20_01551 at 1625.069 kb on - strand, within MPMX20_01551 at 1625.069 kb on - strand, within MPMX20_01551 at 1625.071 kb on + strand, within MPMX20_01551 at 1625.074 kb on - strand, within MPMX20_01551 at 1625.082 kb on - strand, within MPMX20_01551 at 1625.272 kb on - strand, within MPMX20_01551 at 1625.285 kb on - strand, within MPMX20_01551 at 1625.321 kb on + strand, within MPMX20_01551 at 1625.329 kb on - strand, within MPMX20_01551 at 1625.329 kb on - strand, within MPMX20_01551 at 1625.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 1,622,504 + MPMX20_01549 0.57 +1.2 1,622,534 + MPMX20_01549 0.59 +0.2 1,622,534 - MPMX20_01549 0.59 +1.7 1,622,540 - MPMX20_01549 0.59 +1.2 1,622,881 - MPMX20_01549 0.79 +0.7 1,622,913 - MPMX20_01549 0.80 +2.7 1,623,053 + MPMX20_01549 0.88 +2.7 1,623,150 - +0.4 1,623,223 - +0.7 1,623,228 + -0.1 1,623,578 - MPMX20_01550 0.21 +1.2 1,623,670 + MPMX20_01550 0.30 -0.7 1,623,791 - MPMX20_01550 0.42 -0.7 1,623,835 - MPMX20_01550 0.46 +0.4 1,624,267 + MPMX20_01550 0.90 -1.2 1,624,267 + MPMX20_01550 0.90 +0.7 1,624,267 + MPMX20_01550 0.90 +0.2 1,624,275 - -0.1 1,624,275 - -1.3 1,624,275 - -0.8 1,624,323 + -0.7 1,624,323 + +0.2 1,624,323 + +2.7 1,624,325 + +0.2 1,624,333 - +0.2 1,624,336 + -0.6 1,624,343 - -0.1 1,624,454 - -0.4 1,624,467 + +2.3 1,624,524 - -1.5 1,624,526 - +0.4 1,624,583 + +0.5 1,624,647 - +0.4 1,624,695 + -0.8 1,624,716 - -0.1 1,624,819 + +0.6 1,624,819 + -0.7 1,624,819 + -1.5 1,624,827 - -1.0 1,624,827 - +0.1 1,624,831 + -1.2 1,624,832 - +0.6 1,624,839 - -1.0 1,624,841 + +0.1 1,624,841 + -1.7 1,624,841 + -0.6 1,624,842 + -0.3 1,624,842 + -0.2 1,624,843 + +0.6 1,624,851 - +2.1 1,624,855 - +1.2 1,624,856 - -0.1 1,624,863 + -0.5 1,624,923 - -0.8 1,624,923 - +0.7 1,625,019 + MPMX20_01551 0.27 +0.4 1,625,027 - MPMX20_01551 0.29 -1.7 1,625,034 - MPMX20_01551 0.30 -0.5 1,625,061 + MPMX20_01551 0.36 +0.2 1,625,061 + MPMX20_01551 0.36 +0.2 1,625,061 + MPMX20_01551 0.36 +0.7 1,625,069 - MPMX20_01551 0.37 +1.6 1,625,069 - MPMX20_01551 0.37 +0.7 1,625,069 - MPMX20_01551 0.37 -0.8 1,625,069 - MPMX20_01551 0.37 +1.4 1,625,071 + MPMX20_01551 0.38 -0.6 1,625,074 - MPMX20_01551 0.38 +2.7 1,625,082 - MPMX20_01551 0.40 +0.7 1,625,272 - MPMX20_01551 0.78 +0.4 1,625,285 - MPMX20_01551 0.81 +1.3 1,625,321 + MPMX20_01551 0.88 -0.3 1,625,329 - MPMX20_01551 0.90 +1.7 1,625,329 - MPMX20_01551 0.90 -0.5 1,625,349 + -0.4
Or see this region's nucleotide sequence