Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01000 and MPMX20_01001 are separated by 142 nucleotides MPMX20_01001 and MPMX20_01002 are separated by 14 nucleotides MPMX20_01002 and MPMX20_01003 are separated by 48 nucleotides
MPMX20_01000: MPMX20_01000 - Protein translocase subunit SecF, at 1,054,606 to 1,055,577
_01000
MPMX20_01001: MPMX20_01001 - hypothetical protein, at 1,055,720 to 1,056,085
_01001
MPMX20_01002: MPMX20_01002 - hypothetical protein, at 1,056,100 to 1,056,780
_01002
MPMX20_01003: MPMX20_01003 - Nucleoside-specific channel-forming protein Tsx, at 1,056,829 to 1,057,719
_01003
Position (kb)
1055
1056
1057 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1055.662 kb on + strand at 1055.677 kb on + strand at 1055.685 kb on - strand at 1055.800 kb on + strand, within MPMX20_01001 at 1055.806 kb on + strand, within MPMX20_01001 at 1055.807 kb on - strand, within MPMX20_01001 at 1055.814 kb on - strand, within MPMX20_01001 at 1055.903 kb on - strand, within MPMX20_01001 at 1055.904 kb on + strand, within MPMX20_01001 at 1055.914 kb on - strand, within MPMX20_01001 at 1055.965 kb on + strand, within MPMX20_01001 at 1056.040 kb on - strand, within MPMX20_01001 at 1056.078 kb on - strand at 1056.078 kb on - strand at 1056.078 kb on - strand at 1056.097 kb on + strand at 1056.185 kb on + strand, within MPMX20_01002 at 1056.185 kb on + strand, within MPMX20_01002 at 1056.193 kb on - strand, within MPMX20_01002 at 1056.357 kb on + strand, within MPMX20_01002 at 1056.403 kb on + strand, within MPMX20_01002 at 1056.416 kb on - strand, within MPMX20_01002 at 1056.416 kb on - strand, within MPMX20_01002 at 1056.428 kb on + strand, within MPMX20_01002 at 1056.517 kb on - strand, within MPMX20_01002 at 1056.517 kb on - strand, within MPMX20_01002 at 1056.532 kb on + strand, within MPMX20_01002 at 1056.566 kb on - strand, within MPMX20_01002 at 1056.566 kb on - strand, within MPMX20_01002 at 1056.626 kb on - strand, within MPMX20_01002 at 1056.631 kb on - strand, within MPMX20_01002 at 1056.650 kb on + strand, within MPMX20_01002 at 1056.675 kb on - strand, within MPMX20_01002 at 1056.909 kb on - strand at 1056.986 kb on + strand, within MPMX20_01003 at 1056.990 kb on + strand, within MPMX20_01003 at 1056.992 kb on - strand, within MPMX20_01003 at 1057.068 kb on - strand, within MPMX20_01003 at 1057.073 kb on - strand, within MPMX20_01003 at 1057.073 kb on - strand, within MPMX20_01003
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 1,055,662 + -0.4 1,055,677 + -1.2 1,055,685 - +2.0 1,055,800 + MPMX20_01001 0.22 -1.3 1,055,806 + MPMX20_01001 0.23 +0.8 1,055,807 - MPMX20_01001 0.24 -1.3 1,055,814 - MPMX20_01001 0.26 -1.8 1,055,903 - MPMX20_01001 0.50 -2.1 1,055,904 + MPMX20_01001 0.50 -1.6 1,055,914 - MPMX20_01001 0.53 +1.0 1,055,965 + MPMX20_01001 0.67 +0.3 1,056,040 - MPMX20_01001 0.87 -0.6 1,056,078 - -0.8 1,056,078 - +2.8 1,056,078 - -0.2 1,056,097 + -0.5 1,056,185 + MPMX20_01002 0.12 -0.7 1,056,185 + MPMX20_01002 0.12 +0.2 1,056,193 - MPMX20_01002 0.14 +0.1 1,056,357 + MPMX20_01002 0.38 -0.2 1,056,403 + MPMX20_01002 0.44 -0.9 1,056,416 - MPMX20_01002 0.46 +0.2 1,056,416 - MPMX20_01002 0.46 +1.3 1,056,428 + MPMX20_01002 0.48 -1.3 1,056,517 - MPMX20_01002 0.61 -1.7 1,056,517 - MPMX20_01002 0.61 -0.7 1,056,532 + MPMX20_01002 0.63 -1.1 1,056,566 - MPMX20_01002 0.68 +0.3 1,056,566 - MPMX20_01002 0.68 +0.4 1,056,626 - MPMX20_01002 0.77 -0.0 1,056,631 - MPMX20_01002 0.78 -0.1 1,056,650 + MPMX20_01002 0.81 +0.4 1,056,675 - MPMX20_01002 0.84 +1.0 1,056,909 - -0.7 1,056,986 + MPMX20_01003 0.18 -1.9 1,056,990 + MPMX20_01003 0.18 -1.3 1,056,992 - MPMX20_01003 0.18 +1.8 1,057,068 - MPMX20_01003 0.27 -2.6 1,057,073 - MPMX20_01003 0.27 -0.5 1,057,073 - MPMX20_01003 0.27 -0.6
Or see this region's nucleotide sequence