Experiment: TSB plate, 1:1 coculture with Bacillus megaterium TBS_037
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00155 and MPMX20_00156 are separated by 224 nucleotides MPMX20_00156 and MPMX20_00157 are separated by 3 nucleotides MPMX20_00157 and MPMX20_00158 are separated by 4 nucleotides
MPMX20_00155: MPMX20_00155 - 2,3-diketo-L-gulonate reductase, at 158,571 to 159,569
_00155
MPMX20_00156: MPMX20_00156 - DNA-binding transcriptional repressor YiaJ, at 159,794 to 160,612
_00156
MPMX20_00157: MPMX20_00157 - Iron-sulfur protein, at 160,616 to 161,071
_00157
MPMX20_00158: MPMX20_00158 - Valine--pyruvate aminotransferase, at 161,076 to 162,332
_00158
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 158.855 kb on + strand, within MPMX20_00155 at 158.855 kb on + strand, within MPMX20_00155 at 158.855 kb on + strand, within MPMX20_00155 at 158.855 kb on + strand, within MPMX20_00155 at 158.858 kb on - strand, within MPMX20_00155 at 158.880 kb on + strand, within MPMX20_00155 at 158.880 kb on + strand, within MPMX20_00155 at 158.886 kb on + strand, within MPMX20_00155 at 158.886 kb on + strand, within MPMX20_00155 at 158.888 kb on - strand, within MPMX20_00155 at 158.888 kb on - strand, within MPMX20_00155 at 158.888 kb on - strand, within MPMX20_00155 at 158.888 kb on - strand, within MPMX20_00155 at 158.893 kb on - strand, within MPMX20_00155 at 158.961 kb on + strand, within MPMX20_00155 at 158.998 kb on + strand, within MPMX20_00155 at 159.091 kb on + strand, within MPMX20_00155 at 159.099 kb on - strand, within MPMX20_00155 at 159.106 kb on - strand, within MPMX20_00155 at 159.106 kb on - strand, within MPMX20_00155 at 159.111 kb on - strand, within MPMX20_00155 at 159.155 kb on - strand, within MPMX20_00155 at 159.346 kb on - strand, within MPMX20_00155 at 159.415 kb on - strand, within MPMX20_00155 at 159.428 kb on + strand, within MPMX20_00155 at 159.428 kb on + strand, within MPMX20_00155 at 159.428 kb on + strand, within MPMX20_00155 at 159.428 kb on + strand, within MPMX20_00155 at 159.428 kb on + strand, within MPMX20_00155 at 159.434 kb on - strand, within MPMX20_00155 at 159.436 kb on - strand, within MPMX20_00155 at 159.436 kb on - strand, within MPMX20_00155 at 159.445 kb on - strand, within MPMX20_00155 at 159.455 kb on + strand, within MPMX20_00155 at 159.486 kb on + strand at 159.494 kb on - strand at 159.524 kb on + strand at 159.528 kb on - strand at 159.528 kb on - strand at 159.796 kb on - strand at 159.864 kb on + strand at 159.955 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.972 kb on + strand, within MPMX20_00156 at 159.980 kb on - strand, within MPMX20_00156 at 159.980 kb on - strand, within MPMX20_00156 at 159.980 kb on - strand, within MPMX20_00156 at 159.993 kb on - strand, within MPMX20_00156 at 159.995 kb on + strand, within MPMX20_00156 at 160.017 kb on + strand, within MPMX20_00156 at 160.025 kb on - strand, within MPMX20_00156 at 160.066 kb on - strand, within MPMX20_00156 at 160.092 kb on - strand, within MPMX20_00156 at 160.100 kb on - strand, within MPMX20_00156 at 160.107 kb on - strand, within MPMX20_00156 at 160.119 kb on + strand, within MPMX20_00156 at 160.130 kb on + strand, within MPMX20_00156 at 160.165 kb on - strand, within MPMX20_00156 at 160.186 kb on + strand, within MPMX20_00156 at 160.194 kb on + strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.231 kb on - strand, within MPMX20_00156 at 160.245 kb on - strand, within MPMX20_00156 at 160.279 kb on - strand, within MPMX20_00156 at 160.293 kb on - strand, within MPMX20_00156 at 160.314 kb on + strand, within MPMX20_00156 at 160.362 kb on + strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.446 kb on - strand, within MPMX20_00156 at 160.466 kb on + strand, within MPMX20_00156 at 160.543 kb on + strand at 160.590 kb on + strand at 160.590 kb on + strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.665 kb on + strand, within MPMX20_00157 at 160.688 kb on - strand, within MPMX20_00157 at 160.736 kb on - strand, within MPMX20_00157 at 160.756 kb on + strand, within MPMX20_00157 at 160.763 kb on + strand, within MPMX20_00157 at 160.771 kb on - strand, within MPMX20_00157 at 160.823 kb on + strand, within MPMX20_00157 at 160.975 kb on - strand, within MPMX20_00157 at 160.987 kb on - strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 161.020 kb on - strand, within MPMX20_00157 at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.092 kb on + strand at 161.171 kb on - strand at 161.191 kb on + strand at 161.202 kb on + strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.220 kb on - strand, within MPMX20_00158 at 161.411 kb on + strand, within MPMX20_00158 at 161.449 kb on - strand, within MPMX20_00158 at 161.492 kb on + strand, within MPMX20_00158 at 161.500 kb on - strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.595 kb on + strand, within MPMX20_00158
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Bacillus megaterium TBS_037 remove 158,855 + MPMX20_00155 0.28 -0.7 158,855 + MPMX20_00155 0.28 +1.3 158,855 + MPMX20_00155 0.28 -0.7 158,855 + MPMX20_00155 0.28 -0.2 158,858 - MPMX20_00155 0.29 -2.3 158,880 + MPMX20_00155 0.31 +1.8 158,880 + MPMX20_00155 0.31 +1.6 158,886 + MPMX20_00155 0.32 +1.0 158,886 + MPMX20_00155 0.32 -0.1 158,888 - MPMX20_00155 0.32 -1.6 158,888 - MPMX20_00155 0.32 -1.2 158,888 - MPMX20_00155 0.32 -0.7 158,888 - MPMX20_00155 0.32 -0.7 158,893 - MPMX20_00155 0.32 +1.1 158,961 + MPMX20_00155 0.39 +2.2 158,998 + MPMX20_00155 0.43 +1.2 159,091 + MPMX20_00155 0.52 -2.0 159,099 - MPMX20_00155 0.53 -0.0 159,106 - MPMX20_00155 0.54 -0.0 159,106 - MPMX20_00155 0.54 +0.5 159,111 - MPMX20_00155 0.54 -1.8 159,155 - MPMX20_00155 0.58 +0.1 159,346 - MPMX20_00155 0.78 -0.2 159,415 - MPMX20_00155 0.84 -2.0 159,428 + MPMX20_00155 0.86 -0.4 159,428 + MPMX20_00155 0.86 +1.2 159,428 + MPMX20_00155 0.86 +1.8 159,428 + MPMX20_00155 0.86 -1.4 159,428 + MPMX20_00155 0.86 +2.4 159,434 - MPMX20_00155 0.86 +0.2 159,436 - MPMX20_00155 0.87 -2.4 159,436 - MPMX20_00155 0.87 -0.5 159,445 - MPMX20_00155 0.87 -0.4 159,455 + MPMX20_00155 0.88 -0.9 159,486 + -0.7 159,494 - +1.8 159,524 + +0.3 159,528 - -1.7 159,528 - -2.3 159,796 - +0.5 159,864 + -1.8 159,955 + MPMX20_00156 0.20 -2.3 159,972 + MPMX20_00156 0.22 +0.8 159,972 + MPMX20_00156 0.22 +2.8 159,972 + MPMX20_00156 0.22 -1.4 159,972 + MPMX20_00156 0.22 +0.7 159,972 + MPMX20_00156 0.22 -1.5 159,972 + MPMX20_00156 0.22 -0.9 159,972 + MPMX20_00156 0.22 -1.1 159,980 - MPMX20_00156 0.23 -0.6 159,980 - MPMX20_00156 0.23 -1.0 159,980 - MPMX20_00156 0.23 -0.5 159,993 - MPMX20_00156 0.24 -1.2 159,995 + MPMX20_00156 0.25 +2.8 160,017 + MPMX20_00156 0.27 -1.7 160,025 - MPMX20_00156 0.28 -0.0 160,066 - MPMX20_00156 0.33 -1.7 160,092 - MPMX20_00156 0.36 +0.5 160,100 - MPMX20_00156 0.37 +0.5 160,107 - MPMX20_00156 0.38 +2.8 160,119 + MPMX20_00156 0.40 -1.4 160,130 + MPMX20_00156 0.41 -3.5 160,165 - MPMX20_00156 0.45 -1.1 160,186 + MPMX20_00156 0.48 +0.5 160,194 + MPMX20_00156 0.49 +0.6 160,194 - MPMX20_00156 0.49 -0.5 160,194 - MPMX20_00156 0.49 -1.7 160,231 - MPMX20_00156 0.53 -0.2 160,245 - MPMX20_00156 0.55 -0.7 160,279 - MPMX20_00156 0.59 -0.1 160,293 - MPMX20_00156 0.61 -0.9 160,314 + MPMX20_00156 0.63 -2.4 160,362 + MPMX20_00156 0.69 +0.5 160,407 - MPMX20_00156 0.75 -2.9 160,407 - MPMX20_00156 0.75 -1.0 160,446 - MPMX20_00156 0.80 -1.0 160,466 + MPMX20_00156 0.82 -1.5 160,543 + -1.1 160,590 + -0.9 160,590 + -1.5 160,598 - +1.0 160,598 - +0.7 160,598 - -0.0 160,665 + MPMX20_00157 0.11 +0.2 160,688 - MPMX20_00157 0.16 -0.3 160,736 - MPMX20_00157 0.26 -1.4 160,756 + MPMX20_00157 0.31 -0.6 160,763 + MPMX20_00157 0.32 -0.3 160,771 - MPMX20_00157 0.34 +0.2 160,823 + MPMX20_00157 0.45 +0.0 160,975 - MPMX20_00157 0.79 -1.3 160,987 - MPMX20_00157 0.81 -0.5 160,989 + MPMX20_00157 0.82 -0.4 160,989 + MPMX20_00157 0.82 -0.7 160,989 + MPMX20_00157 0.82 +1.8 160,989 + MPMX20_00157 0.82 +2.8 160,989 + MPMX20_00157 0.82 -2.7 160,997 - MPMX20_00157 0.84 +1.5 160,997 - MPMX20_00157 0.84 +0.5 161,020 - MPMX20_00157 0.89 -0.7 161,030 - -0.4 161,030 - -1.3 161,030 - +0.2 161,030 - -1.6 161,092 + -0.8 161,171 - +0.8 161,191 + -0.7 161,202 + MPMX20_00158 0.10 -1.0 161,210 - MPMX20_00158 0.11 -1.0 161,210 - MPMX20_00158 0.11 -0.9 161,220 - MPMX20_00158 0.11 -0.4 161,411 + MPMX20_00158 0.27 +0.6 161,449 - MPMX20_00158 0.30 -1.0 161,492 + MPMX20_00158 0.33 -0.6 161,500 - MPMX20_00158 0.34 -0.0 161,594 + MPMX20_00158 0.41 -1.0 161,594 + MPMX20_00158 0.41 -0.8 161,595 + MPMX20_00158 0.41 +2.2
Or see this region's nucleotide sequence