Experiment: 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03984 and MPMX20_03985 are separated by 12 nucleotides MPMX20_03985 and MPMX20_03986 are separated by 38 nucleotides MPMX20_03986 and MPMX20_03987 overlap by 4 nucleotides MPMX20_03987 and MPMX20_03988 are separated by 11 nucleotides
MPMX20_03984: MPMX20_03984 - hypothetical protein, at 4,179,660 to 4,180,346
_03984
MPMX20_03985: MPMX20_03985 - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,180,359 to 4,181,207
_03985
MPMX20_03986: MPMX20_03986 - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 4,181,246 to 4,181,539
_03986
MPMX20_03987: MPMX20_03987 - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 4,181,536 to 4,182,423
_03987
MPMX20_03988: MPMX20_03988 - Autoinducer 2-binding protein LsrB, at 4,182,435 to 4,183,436
_03988
Position (kb)
4181
4182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4180.257 kb on + strand, within MPMX20_03984 at 4180.281 kb on - strand at 4180.408 kb on + strand at 4180.505 kb on + strand, within MPMX20_03985 at 4180.518 kb on - strand, within MPMX20_03985 at 4180.518 kb on - strand, within MPMX20_03985 at 4180.579 kb on + strand, within MPMX20_03985 at 4180.641 kb on - strand, within MPMX20_03985 at 4180.661 kb on - strand, within MPMX20_03985 at 4180.680 kb on - strand, within MPMX20_03985 at 4180.695 kb on + strand, within MPMX20_03985 at 4180.695 kb on + strand, within MPMX20_03985 at 4180.704 kb on - strand, within MPMX20_03985 at 4180.712 kb on + strand, within MPMX20_03985 at 4180.714 kb on - strand, within MPMX20_03985 at 4180.850 kb on + strand, within MPMX20_03985 at 4180.888 kb on + strand, within MPMX20_03985 at 4180.974 kb on - strand, within MPMX20_03985 at 4181.042 kb on + strand, within MPMX20_03985 at 4181.127 kb on - strand at 4181.152 kb on + strand at 4181.160 kb on - strand at 4181.204 kb on - strand at 4181.223 kb on + strand at 4181.241 kb on + strand at 4181.292 kb on + strand, within MPMX20_03986 at 4181.309 kb on - strand, within MPMX20_03986 at 4181.321 kb on + strand, within MPMX20_03986 at 4181.477 kb on + strand, within MPMX20_03986 at 4181.477 kb on - strand, within MPMX20_03986 at 4181.505 kb on + strand, within MPMX20_03986 at 4181.513 kb on - strand at 4181.564 kb on + strand at 4181.642 kb on + strand, within MPMX20_03987 at 4181.686 kb on + strand, within MPMX20_03987 at 4181.737 kb on + strand, within MPMX20_03987 at 4181.748 kb on + strand, within MPMX20_03987 at 4181.843 kb on + strand, within MPMX20_03987 at 4182.001 kb on - strand, within MPMX20_03987 at 4182.001 kb on - strand, within MPMX20_03987 at 4182.001 kb on - strand, within MPMX20_03987 at 4182.135 kb on + strand, within MPMX20_03987 at 4182.136 kb on - strand, within MPMX20_03987 at 4182.190 kb on - strand, within MPMX20_03987 at 4182.216 kb on + strand, within MPMX20_03987 at 4182.224 kb on - strand, within MPMX20_03987 at 4182.224 kb on - strand, within MPMX20_03987 at 4182.224 kb on - strand, within MPMX20_03987 at 4182.224 kb on - strand, within MPMX20_03987 at 4182.224 kb on - strand, within MPMX20_03987 at 4182.305 kb on + strand, within MPMX20_03987 at 4182.313 kb on - strand, within MPMX20_03987 at 4182.328 kb on - strand, within MPMX20_03987 at 4182.343 kb on + strand at 4182.350 kb on - strand at 4182.350 kb on - strand at 4182.371 kb on + strand at 4182.473 kb on - strand at 4182.491 kb on + strand at 4182.507 kb on - strand at 4182.519 kb on - strand at 4182.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040 remove 4,180,257 + MPMX20_03984 0.87 -0.9 4,180,281 - -1.2 4,180,408 + +0.7 4,180,505 + MPMX20_03985 0.17 -1.8 4,180,518 - MPMX20_03985 0.19 +0.8 4,180,518 - MPMX20_03985 0.19 +0.9 4,180,579 + MPMX20_03985 0.26 +2.2 4,180,641 - MPMX20_03985 0.33 +0.8 4,180,661 - MPMX20_03985 0.36 +1.8 4,180,680 - MPMX20_03985 0.38 -2.4 4,180,695 + MPMX20_03985 0.40 +0.2 4,180,695 + MPMX20_03985 0.40 -0.7 4,180,704 - MPMX20_03985 0.41 +1.7 4,180,712 + MPMX20_03985 0.42 +0.0 4,180,714 - MPMX20_03985 0.42 +0.8 4,180,850 + MPMX20_03985 0.58 +1.7 4,180,888 + MPMX20_03985 0.62 -0.8 4,180,974 - MPMX20_03985 0.72 -0.7 4,181,042 + MPMX20_03985 0.80 +1.1 4,181,127 - +0.1 4,181,152 + -0.5 4,181,160 - +2.5 4,181,204 - +0.9 4,181,223 + -0.3 4,181,241 + +1.8 4,181,292 + MPMX20_03986 0.16 -2.7 4,181,309 - MPMX20_03986 0.21 -1.2 4,181,321 + MPMX20_03986 0.26 -1.9 4,181,477 + MPMX20_03986 0.79 -1.6 4,181,477 - MPMX20_03986 0.79 -3.6 4,181,505 + MPMX20_03986 0.88 -2.6 4,181,513 - -1.8 4,181,564 + -2.3 4,181,642 + MPMX20_03987 0.12 +0.9 4,181,686 + MPMX20_03987 0.17 -2.7 4,181,737 + MPMX20_03987 0.23 -0.9 4,181,748 + MPMX20_03987 0.24 -0.6 4,181,843 + MPMX20_03987 0.35 -2.2 4,182,001 - MPMX20_03987 0.52 +0.6 4,182,001 - MPMX20_03987 0.52 -0.9 4,182,001 - MPMX20_03987 0.52 +3.4 4,182,135 + MPMX20_03987 0.67 -1.7 4,182,136 - MPMX20_03987 0.68 +2.5 4,182,190 - MPMX20_03987 0.74 +2.3 4,182,216 + MPMX20_03987 0.77 +0.9 4,182,224 - MPMX20_03987 0.77 -0.5 4,182,224 - MPMX20_03987 0.77 -1.1 4,182,224 - MPMX20_03987 0.77 -1.7 4,182,224 - MPMX20_03987 0.77 +0.5 4,182,224 - MPMX20_03987 0.77 -0.1 4,182,305 + MPMX20_03987 0.87 -0.3 4,182,313 - MPMX20_03987 0.88 -0.5 4,182,328 - MPMX20_03987 0.89 -0.1 4,182,343 + -1.7 4,182,350 - -2.3 4,182,350 - -0.8 4,182,371 + -3.3 4,182,473 - -2.9 4,182,491 + -0.5 4,182,507 - -0.9 4,182,519 - -2.5 4,182,533 - -0.8
Or see this region's nucleotide sequence