Experiment: 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03251 and MPMX20_03252 are separated by 154 nucleotides MPMX20_03252 and MPMX20_03253 are separated by 28 nucleotides MPMX20_03253 and MPMX20_03254 overlap by 1 nucleotides
MPMX20_03251: MPMX20_03251 - Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, at 3,421,030 to 3,421,935
_03251
MPMX20_03252: MPMX20_03252 - Protein DedA, at 3,422,090 to 3,422,749
_03252
MPMX20_03253: MPMX20_03253 - tRNA pseudouridine synthase A, at 3,422,778 to 3,423,590
_03253
MPMX20_03254: MPMX20_03254 - USG-1 protein, at 3,423,590 to 3,424,603
_03254
Position (kb)
3422
3423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3422.140 kb on + strand at 3422.149 kb on + strand at 3422.247 kb on + strand, within MPMX20_03252 at 3422.247 kb on + strand, within MPMX20_03252 at 3422.247 kb on + strand, within MPMX20_03252 at 3422.255 kb on - strand, within MPMX20_03252 at 3422.255 kb on - strand, within MPMX20_03252 at 3422.255 kb on - strand, within MPMX20_03252 at 3422.255 kb on - strand, within MPMX20_03252 at 3422.255 kb on - strand, within MPMX20_03252 at 3422.460 kb on + strand, within MPMX20_03252 at 3422.468 kb on - strand, within MPMX20_03252 at 3422.483 kb on + strand, within MPMX20_03252 at 3422.648 kb on + strand, within MPMX20_03252 at 3422.664 kb on + strand, within MPMX20_03252 at 3422.673 kb on - strand, within MPMX20_03252 at 3422.689 kb on + strand at 3422.706 kb on + strand at 3422.716 kb on + strand at 3422.771 kb on + strand at 3422.779 kb on - strand at 3422.802 kb on + strand at 3422.827 kb on - strand at 3422.895 kb on + strand, within MPMX20_03253 at 3422.895 kb on + strand, within MPMX20_03253 at 3422.898 kb on - strand, within MPMX20_03253 at 3422.928 kb on - strand, within MPMX20_03253 at 3422.936 kb on + strand, within MPMX20_03253 at 3422.973 kb on + strand, within MPMX20_03253 at 3422.989 kb on - strand, within MPMX20_03253 at 3422.993 kb on - strand, within MPMX20_03253 at 3423.023 kb on + strand, within MPMX20_03253 at 3423.051 kb on - strand, within MPMX20_03253 at 3423.073 kb on + strand, within MPMX20_03253 at 3423.081 kb on - strand, within MPMX20_03253 at 3423.084 kb on + strand, within MPMX20_03253 at 3423.092 kb on - strand, within MPMX20_03253 at 3423.124 kb on + strand, within MPMX20_03253 at 3423.134 kb on + strand, within MPMX20_03253 at 3423.157 kb on + strand, within MPMX20_03253 at 3423.192 kb on + strand, within MPMX20_03253 at 3423.200 kb on - strand, within MPMX20_03253 at 3423.200 kb on - strand, within MPMX20_03253 at 3423.200 kb on - strand, within MPMX20_03253 at 3423.230 kb on - strand, within MPMX20_03253 at 3423.286 kb on + strand, within MPMX20_03253 at 3423.292 kb on - strand, within MPMX20_03253 at 3423.427 kb on - strand, within MPMX20_03253 at 3423.452 kb on + strand, within MPMX20_03253 at 3423.517 kb on + strand at 3423.588 kb on + strand at 3423.588 kb on + strand at 3423.588 kb on + strand at 3423.596 kb on - strand at 3423.715 kb on + strand, within MPMX20_03254
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040 remove 3,422,140 + -2.3 3,422,149 + -0.5 3,422,247 + MPMX20_03252 0.24 +0.4 3,422,247 + MPMX20_03252 0.24 -2.6 3,422,247 + MPMX20_03252 0.24 -2.3 3,422,255 - MPMX20_03252 0.25 -2.5 3,422,255 - MPMX20_03252 0.25 +0.0 3,422,255 - MPMX20_03252 0.25 +3.1 3,422,255 - MPMX20_03252 0.25 -1.8 3,422,255 - MPMX20_03252 0.25 -0.5 3,422,460 + MPMX20_03252 0.56 -2.8 3,422,468 - MPMX20_03252 0.57 +0.4 3,422,483 + MPMX20_03252 0.60 -2.1 3,422,648 + MPMX20_03252 0.85 +1.0 3,422,664 + MPMX20_03252 0.87 -2.0 3,422,673 - MPMX20_03252 0.88 -0.2 3,422,689 + +1.1 3,422,706 + -0.8 3,422,716 + -0.2 3,422,771 + -1.5 3,422,779 - -0.2 3,422,802 + -1.1 3,422,827 - +0.9 3,422,895 + MPMX20_03253 0.14 -2.0 3,422,895 + MPMX20_03253 0.14 -0.2 3,422,898 - MPMX20_03253 0.15 +1.6 3,422,928 - MPMX20_03253 0.18 +0.0 3,422,936 + MPMX20_03253 0.19 +2.2 3,422,973 + MPMX20_03253 0.24 +1.4 3,422,989 - MPMX20_03253 0.26 -0.5 3,422,993 - MPMX20_03253 0.26 -0.5 3,423,023 + MPMX20_03253 0.30 -1.5 3,423,051 - MPMX20_03253 0.34 +2.6 3,423,073 + MPMX20_03253 0.36 -0.0 3,423,081 - MPMX20_03253 0.37 +0.3 3,423,084 + MPMX20_03253 0.38 +1.2 3,423,092 - MPMX20_03253 0.39 -1.6 3,423,124 + MPMX20_03253 0.43 +0.5 3,423,134 + MPMX20_03253 0.44 +0.1 3,423,157 + MPMX20_03253 0.47 -1.5 3,423,192 + MPMX20_03253 0.51 +0.8 3,423,200 - MPMX20_03253 0.52 +0.5 3,423,200 - MPMX20_03253 0.52 -0.5 3,423,200 - MPMX20_03253 0.52 -1.0 3,423,230 - MPMX20_03253 0.56 -2.5 3,423,286 + MPMX20_03253 0.62 +2.6 3,423,292 - MPMX20_03253 0.63 +2.0 3,423,427 - MPMX20_03253 0.80 +0.3 3,423,452 + MPMX20_03253 0.83 -1.2 3,423,517 + -1.8 3,423,588 + -0.8 3,423,588 + -1.0 3,423,588 + -0.7 3,423,596 - -1.6 3,423,715 + MPMX20_03254 0.12 +1.0
Or see this region's nucleotide sequence