Experiment: 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01352 and MPMX20_01353 are separated by 15 nucleotides MPMX20_01353 and MPMX20_01354 are separated by 226 nucleotides MPMX20_01354 and MPMX20_01355 are separated by 14 nucleotides
MPMX20_01352: MPMX20_01352 - Succinate dehydrogenase flavoprotein subunit, at 1,414,492 to 1,416,258
_01352
MPMX20_01353: MPMX20_01353 - Succinate dehydrogenase iron-sulfur subunit, at 1,416,274 to 1,416,990
_01353
MPMX20_01354: MPMX20_01354 - 2-oxoglutarate dehydrogenase E1 component, at 1,417,217 to 1,420,024
_01354
MPMX20_01355: MPMX20_01355 - Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, at 1,420,039 to 1,421,265
_01355
Position (kb)
1417
1418
1419
1420
1421 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1416.386 kb on + strand, within MPMX20_01353 at 1416.394 kb on - strand, within MPMX20_01353 at 1416.446 kb on - strand, within MPMX20_01353 at 1416.492 kb on + strand, within MPMX20_01353 at 1416.514 kb on + strand, within MPMX20_01353 at 1416.680 kb on + strand, within MPMX20_01353 at 1416.680 kb on + strand, within MPMX20_01353 at 1416.707 kb on + strand, within MPMX20_01353 at 1416.724 kb on - strand, within MPMX20_01353 at 1416.724 kb on - strand, within MPMX20_01353 at 1416.762 kb on - strand, within MPMX20_01353 at 1416.836 kb on - strand, within MPMX20_01353 at 1416.914 kb on + strand, within MPMX20_01353 at 1416.919 kb on + strand at 1416.920 kb on + strand at 1416.970 kb on - strand at 1416.983 kb on + strand at 1416.990 kb on + strand at 1417.020 kb on + strand at 1417.020 kb on + strand at 1417.020 kb on + strand at 1417.022 kb on - strand at 1417.027 kb on - strand at 1417.028 kb on - strand at 1417.039 kb on + strand at 1417.040 kb on - strand at 1419.439 kb on + strand, within MPMX20_01354 at 1419.439 kb on + strand, within MPMX20_01354 at 1419.490 kb on + strand, within MPMX20_01354 at 1419.581 kb on + strand, within MPMX20_01354 at 1419.586 kb on + strand, within MPMX20_01354 at 1419.586 kb on + strand, within MPMX20_01354 at 1419.959 kb on + strand at 1419.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040 remove 1,416,386 + MPMX20_01353 0.16 -1.7 1,416,394 - MPMX20_01353 0.17 +0.2 1,416,446 - MPMX20_01353 0.24 +0.5 1,416,492 + MPMX20_01353 0.30 -2.9 1,416,514 + MPMX20_01353 0.33 -1.9 1,416,680 + MPMX20_01353 0.57 -1.6 1,416,680 + MPMX20_01353 0.57 -0.9 1,416,707 + MPMX20_01353 0.60 -1.3 1,416,724 - MPMX20_01353 0.63 -0.6 1,416,724 - MPMX20_01353 0.63 -0.3 1,416,762 - MPMX20_01353 0.68 -1.8 1,416,836 - MPMX20_01353 0.78 -2.4 1,416,914 + MPMX20_01353 0.89 -1.8 1,416,919 + -0.3 1,416,920 + -1.2 1,416,970 - -2.8 1,416,983 + +0.8 1,416,990 + +1.9 1,417,020 + +3.3 1,417,020 + -2.3 1,417,020 + +0.4 1,417,022 - -0.1 1,417,027 - -0.9 1,417,028 - -2.2 1,417,039 + -0.7 1,417,040 - -0.8 1,419,439 + MPMX20_01354 0.79 +0.5 1,419,439 + MPMX20_01354 0.79 -2.1 1,419,490 + MPMX20_01354 0.81 -1.6 1,419,581 + MPMX20_01354 0.84 -0.3 1,419,586 + MPMX20_01354 0.84 +1.5 1,419,586 + MPMX20_01354 0.84 +0.9 1,419,959 + -1.5 1,419,992 + -0.9
Or see this region's nucleotide sequence