Experiment: 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00084 and MPMX20_00085 are separated by 3 nucleotides MPMX20_00085 and MPMX20_00086 are separated by 4 nucleotides MPMX20_00086 and MPMX20_00087 are separated by 19 nucleotides MPMX20_00087 and MPMX20_00088 are separated by 54 nucleotides
MPMX20_00084: MPMX20_00084 - ATP-dependent DNA helicase RecG, at 85,957 to 88,038
_00084
MPMX20_00085: MPMX20_00085 - tRNA (guanosine(18)-2'-O)-methyltransferase, at 88,042 to 88,731
_00085
MPMX20_00086: MPMX20_00086 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 88,736 to 90,850
_00086
MPMX20_00087: MPMX20_00087 - DNA-directed RNA polymerase subunit omega, at 90,870 to 91,145
_00087
MPMX20_00088: MPMX20_00088 - Guanylate kinase, at 91,200 to 91,823
_00088
Position (kb) 88
89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 87.792 kb on - strand, within MPMX20_00084 at 87.794 kb on + strand, within MPMX20_00084 at 87.816 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.829 kb on + strand, within MPMX20_00084 at 87.837 kb on - strand at 87.837 kb on - strand at 88.062 kb on - strand at 88.152 kb on - strand, within MPMX20_00085 at 88.256 kb on - strand, within MPMX20_00085 at 88.284 kb on + strand, within MPMX20_00085 at 88.390 kb on + strand, within MPMX20_00085 at 88.448 kb on - strand, within MPMX20_00085 at 88.599 kb on + strand, within MPMX20_00085 at 88.601 kb on + strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.607 kb on - strand, within MPMX20_00085 at 88.609 kb on - strand, within MPMX20_00085 at 88.636 kb on + strand, within MPMX20_00085 at 88.656 kb on + strand, within MPMX20_00085 at 88.656 kb on + strand, within MPMX20_00085 at 88.687 kb on - strand at 88.922 kb on + strand at 88.987 kb on + strand, within MPMX20_00086 at 89.012 kb on - strand, within MPMX20_00086 at 89.081 kb on - strand, within MPMX20_00086 at 89.113 kb on + strand, within MPMX20_00086 at 89.146 kb on - strand, within MPMX20_00086 at 89.146 kb on - strand, within MPMX20_00086 at 89.420 kb on - strand, within MPMX20_00086 at 90.887 kb on - strand at 90.887 kb on - strand at 90.977 kb on - strand, within MPMX20_00087 at 91.056 kb on + strand, within MPMX20_00087 at 91.059 kb on - strand, within MPMX20_00087 at 91.064 kb on - strand, within MPMX20_00087 at 91.064 kb on - strand, within MPMX20_00087 at 91.099 kb on - strand, within MPMX20_00087 at 91.099 kb on - strand, within MPMX20_00087 at 91.122 kb on + strand at 91.122 kb on + strand at 91.122 kb on + strand at 91.130 kb on - strand at 91.130 kb on - strand at 91.186 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, 1:1 coculture with Bacillus zanthoxyli TBS_040 remove 87,792 - MPMX20_00084 0.88 -0.4 87,794 + MPMX20_00084 0.88 +0.1 87,816 + MPMX20_00084 0.89 +0.3 87,829 + MPMX20_00084 0.90 -3.2 87,829 + MPMX20_00084 0.90 -1.6 87,829 + MPMX20_00084 0.90 -1.6 87,837 - -1.4 87,837 - -0.6 88,062 - +1.1 88,152 - MPMX20_00085 0.16 -0.3 88,256 - MPMX20_00085 0.31 -0.2 88,284 + MPMX20_00085 0.35 +0.2 88,390 + MPMX20_00085 0.50 +0.8 88,448 - MPMX20_00085 0.59 +1.7 88,599 + MPMX20_00085 0.81 +2.4 88,601 + MPMX20_00085 0.81 -0.8 88,607 - MPMX20_00085 0.82 -0.1 88,607 - MPMX20_00085 0.82 +0.0 88,607 - MPMX20_00085 0.82 -1.1 88,607 - MPMX20_00085 0.82 -1.7 88,607 - MPMX20_00085 0.82 -1.4 88,609 - MPMX20_00085 0.82 -1.5 88,636 + MPMX20_00085 0.86 -1.4 88,656 + MPMX20_00085 0.89 -0.1 88,656 + MPMX20_00085 0.89 +2.4 88,687 - -0.8 88,922 + -1.2 88,987 + MPMX20_00086 0.12 +0.2 89,012 - MPMX20_00086 0.13 -3.1 89,081 - MPMX20_00086 0.16 -1.9 89,113 + MPMX20_00086 0.18 -1.4 89,146 - MPMX20_00086 0.19 -0.8 89,146 - MPMX20_00086 0.19 -2.3 89,420 - MPMX20_00086 0.32 -1.8 90,887 - +0.8 90,887 - -1.9 90,977 - MPMX20_00087 0.39 +0.1 91,056 + MPMX20_00087 0.67 +0.4 91,059 - MPMX20_00087 0.68 -1.3 91,064 - MPMX20_00087 0.70 +0.8 91,064 - MPMX20_00087 0.70 -1.3 91,099 - MPMX20_00087 0.83 -0.4 91,099 - MPMX20_00087 0.83 +1.4 91,122 + -1.2 91,122 + +0.1 91,122 + +0.4 91,130 - -0.4 91,130 - +0.8 91,186 + -0.4
Or see this region's nucleotide sequence