Experiment: M9 plate, 1:1 coculture with Brevibacillus agri TBS_043
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00425 and MPMX20_00426 are separated by 40 nucleotides MPMX20_00426 and MPMX20_00427 are separated by 55 nucleotides MPMX20_00427 and MPMX20_00428 are separated by 108 nucleotides MPMX20_00428 and MPMX20_00429 are separated by 38 nucleotides
MPMX20_00425: MPMX20_00425 - L-lysine 2,3-aminomutase, at 441,442 to 442,470
_00425
MPMX20_00426: MPMX20_00426 - Elongation factor P, at 442,511 to 443,077
_00426
MPMX20_00427: MPMX20_00427 - hypothetical protein, at 443,133 to 443,267
_00427
MPMX20_00428: MPMX20_00428 - hypothetical protein, at 443,376 to 443,522
_00428
MPMX20_00429: MPMX20_00429 - Transcriptional regulatory protein RcsA, at 443,561 to 444,160
_00429
Position (kb) 443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 442.185 kb on - strand, within MPMX20_00425 at 443.164 kb on + strand, within MPMX20_00427 at 443.169 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.195 kb on + strand, within MPMX20_00427 at 443.197 kb on + strand, within MPMX20_00427 at 443.203 kb on - strand, within MPMX20_00427 at 443.262 kb on + strand at 443.262 kb on + strand at 443.341 kb on + strand at 443.344 kb on - strand at 443.349 kb on - strand at 443.371 kb on + strand at 443.409 kb on + strand, within MPMX20_00428 at 443.436 kb on + strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.463 kb on - strand, within MPMX20_00428 at 443.506 kb on + strand, within MPMX20_00428 at 443.514 kb on - strand at 443.516 kb on + strand at 443.534 kb on + strand at 443.539 kb on + strand at 443.550 kb on + strand at 443.642 kb on + strand, within MPMX20_00429 at 443.642 kb on + strand, within MPMX20_00429 at 443.642 kb on + strand, within MPMX20_00429 at 443.642 kb on + strand, within MPMX20_00429 at 443.650 kb on - strand, within MPMX20_00429 at 443.650 kb on - strand, within MPMX20_00429 at 443.650 kb on - strand, within MPMX20_00429 at 443.651 kb on - strand, within MPMX20_00429 at 443.669 kb on + strand, within MPMX20_00429 at 443.696 kb on + strand, within MPMX20_00429 at 443.700 kb on + strand, within MPMX20_00429 at 443.709 kb on - strand, within MPMX20_00429 at 443.722 kb on - strand, within MPMX20_00429 at 443.764 kb on + strand, within MPMX20_00429 at 443.764 kb on + strand, within MPMX20_00429 at 443.772 kb on + strand, within MPMX20_00429 at 443.772 kb on - strand, within MPMX20_00429 at 443.803 kb on - strand, within MPMX20_00429 at 443.808 kb on + strand, within MPMX20_00429 at 443.808 kb on + strand, within MPMX20_00429 at 443.808 kb on + strand, within MPMX20_00429 at 443.808 kb on + strand, within MPMX20_00429 at 443.808 kb on + strand, within MPMX20_00429 at 443.816 kb on - strand, within MPMX20_00429 at 443.816 kb on - strand, within MPMX20_00429 at 443.826 kb on - strand, within MPMX20_00429 at 443.826 kb on - strand, within MPMX20_00429 at 443.832 kb on + strand, within MPMX20_00429 at 443.834 kb on + strand, within MPMX20_00429 at 443.842 kb on + strand, within MPMX20_00429 at 443.842 kb on + strand, within MPMX20_00429 at 443.850 kb on - strand, within MPMX20_00429 at 443.850 kb on - strand, within MPMX20_00429 at 443.888 kb on + strand, within MPMX20_00429 at 443.888 kb on + strand, within MPMX20_00429 at 443.896 kb on - strand, within MPMX20_00429 at 443.896 kb on - strand, within MPMX20_00429 at 443.896 kb on - strand, within MPMX20_00429 at 443.896 kb on - strand, within MPMX20_00429 at 443.920 kb on + strand, within MPMX20_00429 at 443.946 kb on - strand, within MPMX20_00429 at 443.946 kb on - strand, within MPMX20_00429 at 443.946 kb on - strand, within MPMX20_00429 at 443.953 kb on + strand, within MPMX20_00429 at 443.962 kb on + strand, within MPMX20_00429 at 443.968 kb on - strand, within MPMX20_00429 at 443.970 kb on - strand, within MPMX20_00429 at 443.971 kb on - strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.974 kb on + strand, within MPMX20_00429 at 443.982 kb on - strand, within MPMX20_00429 at 443.982 kb on - strand, within MPMX20_00429 at 443.982 kb on - strand, within MPMX20_00429 at 443.989 kb on + strand, within MPMX20_00429 at 443.989 kb on + strand, within MPMX20_00429 at 443.989 kb on + strand, within MPMX20_00429 at 443.989 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.994 kb on + strand, within MPMX20_00429 at 443.997 kb on - strand, within MPMX20_00429 at 443.997 kb on - strand, within MPMX20_00429 at 443.997 kb on - strand, within MPMX20_00429 at 443.997 kb on - strand, within MPMX20_00429 at 443.997 kb on - strand, within MPMX20_00429 at 444.002 kb on - strand, within MPMX20_00429 at 444.002 kb on - strand, within MPMX20_00429 at 444.036 kb on - strand, within MPMX20_00429 at 444.067 kb on - strand, within MPMX20_00429 at 444.106 kb on + strand at 444.114 kb on - strand at 444.122 kb on + strand at 444.184 kb on - strand at 444.200 kb on + strand at 444.205 kb on + strand at 444.224 kb on + strand at 444.242 kb on + strand at 444.242 kb on + strand at 444.250 kb on - strand at 444.250 kb on - strand at 444.250 kb on - strand at 444.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Brevibacillus agri TBS_043 remove 442,185 - MPMX20_00425 0.72 +0.5 443,164 + MPMX20_00427 0.23 -1.0 443,169 + MPMX20_00427 0.27 -0.8 443,174 + MPMX20_00427 0.30 +7.6 443,174 + MPMX20_00427 0.30 -2.6 443,174 + MPMX20_00427 0.30 +0.2 443,174 + MPMX20_00427 0.30 +1.2 443,174 + MPMX20_00427 0.30 +0.2 443,174 + MPMX20_00427 0.30 -0.9 443,174 + MPMX20_00427 0.30 -0.9 443,182 - MPMX20_00427 0.36 -0.5 443,182 - MPMX20_00427 0.36 +2.8 443,182 - MPMX20_00427 0.36 -1.5 443,182 - MPMX20_00427 0.36 +0.6 443,182 - MPMX20_00427 0.36 +0.2 443,195 + MPMX20_00427 0.46 -1.0 443,197 + MPMX20_00427 0.47 +0.4 443,203 - MPMX20_00427 0.52 +0.8 443,262 + -0.3 443,262 + +1.1 443,341 + -0.1 443,344 - +2.7 443,349 - +2.1 443,371 + -1.8 443,409 + MPMX20_00428 0.22 -0.7 443,436 + MPMX20_00428 0.41 -0.9 443,446 - MPMX20_00428 0.48 -2.4 443,446 - MPMX20_00428 0.48 +2.1 443,446 - MPMX20_00428 0.48 +1.2 443,463 - MPMX20_00428 0.59 +5.8 443,506 + MPMX20_00428 0.88 +2.1 443,514 - +1.6 443,516 + -0.4 443,534 + -1.6 443,539 + +1.1 443,550 + -1.4 443,642 + MPMX20_00429 0.14 -0.4 443,642 + MPMX20_00429 0.14 +2.4 443,642 + MPMX20_00429 0.14 +0.9 443,642 + MPMX20_00429 0.14 +0.3 443,650 - MPMX20_00429 0.15 +0.5 443,650 - MPMX20_00429 0.15 +0.1 443,650 - MPMX20_00429 0.15 -0.5 443,651 - MPMX20_00429 0.15 -2.9 443,669 + MPMX20_00429 0.18 +1.1 443,696 + MPMX20_00429 0.23 -0.9 443,700 + MPMX20_00429 0.23 +2.5 443,709 - MPMX20_00429 0.25 -0.4 443,722 - MPMX20_00429 0.27 +4.1 443,764 + MPMX20_00429 0.34 +0.1 443,764 + MPMX20_00429 0.34 +0.2 443,772 + MPMX20_00429 0.35 -0.2 443,772 - MPMX20_00429 0.35 -0.7 443,803 - MPMX20_00429 0.40 +1.1 443,808 + MPMX20_00429 0.41 -2.0 443,808 + MPMX20_00429 0.41 +6.2 443,808 + MPMX20_00429 0.41 -0.2 443,808 + MPMX20_00429 0.41 +1.0 443,808 + MPMX20_00429 0.41 +1.5 443,816 - MPMX20_00429 0.42 +0.5 443,816 - MPMX20_00429 0.42 +0.8 443,826 - MPMX20_00429 0.44 -0.1 443,826 - MPMX20_00429 0.44 -0.2 443,832 + MPMX20_00429 0.45 -2.5 443,834 + MPMX20_00429 0.46 +1.8 443,842 + MPMX20_00429 0.47 -0.7 443,842 + MPMX20_00429 0.47 +0.5 443,850 - MPMX20_00429 0.48 +0.6 443,850 - MPMX20_00429 0.48 -0.8 443,888 + MPMX20_00429 0.55 +1.5 443,888 + MPMX20_00429 0.55 +1.1 443,896 - MPMX20_00429 0.56 +5.8 443,896 - MPMX20_00429 0.56 -0.2 443,896 - MPMX20_00429 0.56 -0.2 443,896 - MPMX20_00429 0.56 +1.1 443,920 + MPMX20_00429 0.60 -0.8 443,946 - MPMX20_00429 0.64 +1.9 443,946 - MPMX20_00429 0.64 +2.8 443,946 - MPMX20_00429 0.64 +1.1 443,953 + MPMX20_00429 0.65 +1.8 443,962 + MPMX20_00429 0.67 +1.9 443,968 - MPMX20_00429 0.68 -2.2 443,970 - MPMX20_00429 0.68 +1.4 443,971 - MPMX20_00429 0.68 +2.7 443,974 + MPMX20_00429 0.69 +0.5 443,974 + MPMX20_00429 0.69 +1.6 443,974 + MPMX20_00429 0.69 +2.2 443,974 + MPMX20_00429 0.69 -1.2 443,974 + MPMX20_00429 0.69 +0.5 443,974 + MPMX20_00429 0.69 +2.1 443,982 - MPMX20_00429 0.70 -0.3 443,982 - MPMX20_00429 0.70 -2.7 443,982 - MPMX20_00429 0.70 +2.8 443,989 + MPMX20_00429 0.71 +1.1 443,989 + MPMX20_00429 0.71 -1.4 443,989 + MPMX20_00429 0.71 +0.5 443,989 + MPMX20_00429 0.71 +0.7 443,994 + MPMX20_00429 0.72 +1.5 443,994 + MPMX20_00429 0.72 +0.5 443,994 + MPMX20_00429 0.72 +1.8 443,994 + MPMX20_00429 0.72 +0.8 443,994 + MPMX20_00429 0.72 -1.2 443,994 + MPMX20_00429 0.72 +0.2 443,994 + MPMX20_00429 0.72 +1.6 443,997 - MPMX20_00429 0.73 +1.7 443,997 - MPMX20_00429 0.73 -0.7 443,997 - MPMX20_00429 0.73 +0.1 443,997 - MPMX20_00429 0.73 +1.8 443,997 - MPMX20_00429 0.73 -1.2 444,002 - MPMX20_00429 0.73 -1.7 444,002 - MPMX20_00429 0.73 +0.1 444,036 - MPMX20_00429 0.79 +0.3 444,067 - MPMX20_00429 0.84 +2.2 444,106 + -0.9 444,114 - +0.2 444,122 + -0.4 444,184 - -0.8 444,200 + +1.1 444,205 + -0.5 444,224 + +1.0 444,242 + +0.0 444,242 + -1.4 444,250 - +0.6 444,250 - +5.8 444,250 - -1.0 444,250 - -0.9
Or see this region's nucleotide sequence