Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS06250 and ABIE51_RS06255 are separated by 76 nucleotides ABIE51_RS06255 and ABIE51_RS06260 are separated by 13 nucleotides ABIE51_RS06260 and ABIE51_RS06265 overlap by 4 nucleotides
ABIE51_RS06250: ABIE51_RS06250 - imidazolonepropionase, at 1,334,639 to 1,335,865
_RS06250
ABIE51_RS06255: ABIE51_RS06255 - formimidoylglutamate deiminase, at 1,335,942 to 1,337,291
_RS06255
ABIE51_RS06260: ABIE51_RS06260 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 1,337,305 to 1,337,805
_RS06260
ABIE51_RS06265: ABIE51_RS06265 - DNA-3-methyladenine glycosylase 2, at 1,337,802 to 1,339,385
_RS06265
Position (kb)
1335
1336
1337
1338 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1335.285 kb on + strand, within ABIE51_RS06250 at 1335.286 kb on - strand, within ABIE51_RS06250 at 1335.286 kb on - strand, within ABIE51_RS06250 at 1335.286 kb on - strand, within ABIE51_RS06250 at 1335.337 kb on - strand, within ABIE51_RS06250 at 1335.337 kb on - strand, within ABIE51_RS06250 at 1335.337 kb on - strand, within ABIE51_RS06250 at 1335.570 kb on + strand, within ABIE51_RS06250 at 1335.735 kb on + strand, within ABIE51_RS06250 at 1335.735 kb on + strand, within ABIE51_RS06250 at 1335.736 kb on - strand, within ABIE51_RS06250 at 1335.736 kb on - strand, within ABIE51_RS06250 at 1335.736 kb on - strand, within ABIE51_RS06250 at 1335.736 kb on - strand, within ABIE51_RS06250 at 1335.847 kb on + strand at 1335.847 kb on + strand at 1335.847 kb on + strand at 1335.847 kb on + strand at 1335.847 kb on + strand at 1335.848 kb on - strand at 1335.848 kb on - strand at 1335.878 kb on + strand at 1336.186 kb on - strand, within ABIE51_RS06255 at 1336.186 kb on - strand, within ABIE51_RS06255 at 1336.186 kb on - strand, within ABIE51_RS06255 at 1336.186 kb on - strand, within ABIE51_RS06255 at 1336.242 kb on + strand, within ABIE51_RS06255 at 1336.243 kb on - strand, within ABIE51_RS06255 at 1336.290 kb on + strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.291 kb on - strand, within ABIE51_RS06255 at 1336.321 kb on - strand, within ABIE51_RS06255 at 1336.321 kb on - strand, within ABIE51_RS06255 at 1336.404 kb on + strand, within ABIE51_RS06255 at 1336.404 kb on + strand, within ABIE51_RS06255 at 1336.404 kb on + strand, within ABIE51_RS06255 at 1336.404 kb on + strand, within ABIE51_RS06255 at 1336.405 kb on - strand, within ABIE51_RS06255 at 1336.405 kb on - strand, within ABIE51_RS06255 at 1336.405 kb on - strand, within ABIE51_RS06255 at 1336.405 kb on - strand, within ABIE51_RS06255 at 1336.476 kb on + strand, within ABIE51_RS06255 at 1336.477 kb on - strand, within ABIE51_RS06255 at 1336.477 kb on - strand, within ABIE51_RS06255 at 1336.477 kb on - strand, within ABIE51_RS06255 at 1336.477 kb on - strand, within ABIE51_RS06255 at 1337.077 kb on - strand, within ABIE51_RS06255 at 1337.077 kb on - strand, within ABIE51_RS06255 at 1337.190 kb on + strand at 1337.190 kb on + strand at 1337.190 kb on + strand at 1337.191 kb on - strand at 1337.191 kb on - strand at 1337.516 kb on + strand, within ABIE51_RS06260 at 1337.516 kb on + strand, within ABIE51_RS06260 at 1337.517 kb on - strand, within ABIE51_RS06260 at 1337.517 kb on - strand, within ABIE51_RS06260 at 1337.630 kb on + strand, within ABIE51_RS06260 at 1337.795 kb on + strand at 1337.972 kb on + strand, within ABIE51_RS06265 at 1337.972 kb on + strand, within ABIE51_RS06265 at 1337.973 kb on - strand, within ABIE51_RS06265 at 1337.973 kb on - strand, within ABIE51_RS06265 at 1337.973 kb on - strand, within ABIE51_RS06265 at 1338.061 kb on + strand, within ABIE51_RS06265 at 1338.061 kb on + strand, within ABIE51_RS06265 at 1338.062 kb on - strand, within ABIE51_RS06265 at 1338.062 kb on - strand, within ABIE51_RS06265 at 1338.081 kb on + strand, within ABIE51_RS06265
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,335,285 + ABIE51_RS06250 0.53 -2.8 1,335,286 - ABIE51_RS06250 0.53 -4.1 1,335,286 - ABIE51_RS06250 0.53 -2.6 1,335,286 - ABIE51_RS06250 0.53 -3.1 1,335,337 - ABIE51_RS06250 0.57 -2.1 1,335,337 - ABIE51_RS06250 0.57 -4.9 1,335,337 - ABIE51_RS06250 0.57 -4.2 1,335,570 + ABIE51_RS06250 0.76 -2.9 1,335,735 + ABIE51_RS06250 0.89 -5.0 1,335,735 + ABIE51_RS06250 0.89 -4.4 1,335,736 - ABIE51_RS06250 0.89 -4.0 1,335,736 - ABIE51_RS06250 0.89 -4.3 1,335,736 - ABIE51_RS06250 0.89 -4.0 1,335,736 - ABIE51_RS06250 0.89 -4.0 1,335,847 + -1.6 1,335,847 + -1.9 1,335,847 + -1.5 1,335,847 + -2.3 1,335,847 + +0.2 1,335,848 - -0.4 1,335,848 - -1.1 1,335,878 + -0.7 1,336,186 - ABIE51_RS06255 0.18 -3.9 1,336,186 - ABIE51_RS06255 0.18 -1.7 1,336,186 - ABIE51_RS06255 0.18 -3.2 1,336,186 - ABIE51_RS06255 0.18 -2.9 1,336,242 + ABIE51_RS06255 0.22 -2.8 1,336,243 - ABIE51_RS06255 0.22 -3.7 1,336,290 + ABIE51_RS06255 0.26 -3.1 1,336,291 - ABIE51_RS06255 0.26 -2.0 1,336,291 - ABIE51_RS06255 0.26 -1.7 1,336,291 - ABIE51_RS06255 0.26 -3.2 1,336,291 - ABIE51_RS06255 0.26 -3.3 1,336,291 - ABIE51_RS06255 0.26 -2.3 1,336,291 - ABIE51_RS06255 0.26 -0.8 1,336,321 - ABIE51_RS06255 0.28 -1.3 1,336,321 - ABIE51_RS06255 0.28 -1.1 1,336,404 + ABIE51_RS06255 0.34 -3.2 1,336,404 + ABIE51_RS06255 0.34 -2.1 1,336,404 + ABIE51_RS06255 0.34 -4.2 1,336,404 + ABIE51_RS06255 0.34 -2.4 1,336,405 - ABIE51_RS06255 0.34 -3.7 1,336,405 - ABIE51_RS06255 0.34 -1.7 1,336,405 - ABIE51_RS06255 0.34 -2.7 1,336,405 - ABIE51_RS06255 0.34 -3.1 1,336,476 + ABIE51_RS06255 0.40 -2.6 1,336,477 - ABIE51_RS06255 0.40 -2.1 1,336,477 - ABIE51_RS06255 0.40 -1.6 1,336,477 - ABIE51_RS06255 0.40 -3.2 1,336,477 - ABIE51_RS06255 0.40 -0.7 1,337,077 - ABIE51_RS06255 0.84 -3.7 1,337,077 - ABIE51_RS06255 0.84 -4.3 1,337,190 + -2.9 1,337,190 + -2.4 1,337,190 + -1.6 1,337,191 - -2.3 1,337,191 - -2.1 1,337,516 + ABIE51_RS06260 0.42 +0.4 1,337,516 + ABIE51_RS06260 0.42 +0.0 1,337,517 - ABIE51_RS06260 0.42 +0.3 1,337,517 - ABIE51_RS06260 0.42 -0.2 1,337,630 + ABIE51_RS06260 0.65 +0.7 1,337,795 + -0.2 1,337,972 + ABIE51_RS06265 0.11 -0.2 1,337,972 + ABIE51_RS06265 0.11 +0.2 1,337,973 - ABIE51_RS06265 0.11 -0.4 1,337,973 - ABIE51_RS06265 0.11 +0.7 1,337,973 - ABIE51_RS06265 0.11 +0.2 1,338,061 + ABIE51_RS06265 0.16 +0.1 1,338,061 + ABIE51_RS06265 0.16 +0.3 1,338,062 - ABIE51_RS06265 0.16 -1.7 1,338,062 - ABIE51_RS06265 0.16 -0.2 1,338,081 + ABIE51_RS06265 0.18 -0.1
Or see this region's nucleotide sequence