Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS09420 and ABIE51_RS09425 overlap by 4 nucleotides ABIE51_RS09425 and ABIE51_RS09430 are separated by 105 nucleotides ABIE51_RS09430 and ABIE51_RS09435 overlap by 1 nucleotides
ABIE51_RS09420: ABIE51_RS09420 - type IV pilus biogenesis/stability protein PilW, at 2,031,118 to 2,031,954
_RS09420
ABIE51_RS09425: ABIE51_RS09425 - helix-turn-helix domain-containing protein, at 2,031,951 to 2,032,754
_RS09425
ABIE51_RS09430: ABIE51_RS09430 - tetratricopeptide repeat protein, at 2,032,860 to 2,033,492
_RS09430
ABIE51_RS09435: ABIE51_RS09435 - outer membrane protein assembly factor BamB, at 2,033,492 to 2,034,691
_RS09435
Position (kb)
2032
2033
2034 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.883 kb on + strand at 2031.884 kb on - strand at 2031.884 kb on - strand at 2031.884 kb on - strand at 2031.884 kb on - strand at 2031.884 kb on - strand at 2031.884 kb on - strand at 2032.599 kb on + strand, within ABIE51_RS09425 at 2032.599 kb on + strand, within ABIE51_RS09425 at 2032.599 kb on + strand, within ABIE51_RS09425 at 2032.599 kb on + strand, within ABIE51_RS09425 at 2032.600 kb on - strand, within ABIE51_RS09425 at 2032.600 kb on - strand, within ABIE51_RS09425 at 2032.600 kb on - strand, within ABIE51_RS09425 at 2032.600 kb on - strand, within ABIE51_RS09425 at 2032.715 kb on + strand at 2032.715 kb on + strand at 2032.715 kb on + strand at 2032.715 kb on + strand at 2032.716 kb on - strand at 2032.716 kb on - strand at 2032.716 kb on - strand at 2032.716 kb on - strand at 2032.716 kb on - strand at 2032.720 kb on + strand at 2032.720 kb on + strand at 2032.720 kb on + strand at 2032.721 kb on - strand at 2032.721 kb on - strand at 2032.763 kb on - strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.808 kb on + strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.809 kb on - strand at 2032.855 kb on + strand at 2033.034 kb on + strand, within ABIE51_RS09430 at 2033.034 kb on + strand, within ABIE51_RS09430 at 2033.034 kb on + strand, within ABIE51_RS09430 at 2033.035 kb on - strand, within ABIE51_RS09430 at 2033.115 kb on + strand, within ABIE51_RS09430 at 2033.115 kb on + strand, within ABIE51_RS09430 at 2033.116 kb on - strand, within ABIE51_RS09430 at 2033.116 kb on - strand, within ABIE51_RS09430 at 2033.382 kb on + strand, within ABIE51_RS09430 at 2033.382 kb on + strand, within ABIE51_RS09430 at 2033.382 kb on + strand, within ABIE51_RS09430 at 2033.382 kb on + strand, within ABIE51_RS09430 at 2033.383 kb on - strand, within ABIE51_RS09430 at 2033.383 kb on - strand, within ABIE51_RS09430
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 2,031,883 + -0.2 2,031,883 + +1.3 2,031,883 + +0.1 2,031,883 + +1.2 2,031,883 + +0.6 2,031,883 + +0.1 2,031,883 + +0.1 2,031,883 + -0.1 2,031,884 - -1.9 2,031,884 - -2.5 2,031,884 - -1.7 2,031,884 - -2.7 2,031,884 - -1.7 2,031,884 - -1.7 2,032,599 + ABIE51_RS09425 0.81 -0.1 2,032,599 + ABIE51_RS09425 0.81 -1.7 2,032,599 + ABIE51_RS09425 0.81 -1.1 2,032,599 + ABIE51_RS09425 0.81 -2.1 2,032,600 - ABIE51_RS09425 0.81 -1.7 2,032,600 - ABIE51_RS09425 0.81 -1.7 2,032,600 - ABIE51_RS09425 0.81 -1.1 2,032,600 - ABIE51_RS09425 0.81 -1.1 2,032,715 + -1.1 2,032,715 + -1.7 2,032,715 + -2.5 2,032,715 + -2.5 2,032,716 - -1.1 2,032,716 - -2.7 2,032,716 - -0.1 2,032,716 - -1.5 2,032,716 - -1.1 2,032,720 + -3.0 2,032,720 + -1.7 2,032,720 + -1.7 2,032,721 - -0.1 2,032,721 - -2.7 2,032,763 - -3.5 2,032,808 + +0.0 2,032,808 + -0.4 2,032,808 + -0.1 2,032,808 + +0.2 2,032,808 + +0.3 2,032,808 + +0.5 2,032,808 + +0.8 2,032,808 + +5.5 2,032,808 + -0.2 2,032,809 - -3.9 2,032,809 - -1.2 2,032,809 - -1.7 2,032,809 - -5.1 2,032,809 - -3.2 2,032,809 - -4.2 2,032,809 - -6.1 2,032,809 - -3.5 2,032,855 + +0.3 2,033,034 + ABIE51_RS09430 0.27 -2.0 2,033,034 + ABIE51_RS09430 0.27 -1.7 2,033,034 + ABIE51_RS09430 0.27 +2.1 2,033,035 - ABIE51_RS09430 0.28 -1.2 2,033,115 + ABIE51_RS09430 0.40 -2.2 2,033,115 + ABIE51_RS09430 0.40 -1.9 2,033,116 - ABIE51_RS09430 0.40 -3.4 2,033,116 - ABIE51_RS09430 0.40 -1.7 2,033,382 + ABIE51_RS09430 0.82 -2.5 2,033,382 + ABIE51_RS09430 0.82 -2.6 2,033,382 + ABIE51_RS09430 0.82 -2.6 2,033,382 + ABIE51_RS09430 0.82 -3.6 2,033,383 - ABIE51_RS09430 0.83 -3.2 2,033,383 - ABIE51_RS09430 0.83 -3.6
Or see this region's nucleotide sequence