Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4060 and Pf6N2E2_4061 are separated by 30 nucleotides Pf6N2E2_4061 and Pf6N2E2_4062 overlap by 4 nucleotides Pf6N2E2_4062 and Pf6N2E2_4063 overlap by 2 nucleotides Pf6N2E2_4063 and Pf6N2E2_4064 overlap by 1 nucleotides
Pf6N2E2_4060 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 4,737,038 to 4,738,198
_4060
Pf6N2E2_4061 - Rubredoxin, at 4,738,229 to 4,738,396
_4061
Pf6N2E2_4062 - hypothetical protein, at 4,738,393 to 4,738,548
_4062
Pf6N2E2_4063 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 4,738,547 to 4,739,152
_4063
Pf6N2E2_4064 - 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39), at 4,739,152 to 4,740,042
_4064
Position (kb)
4738
4739 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4737.258 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.330 kb on + strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.331 kb on - strand, within Pf6N2E2_4060 at 4737.375 kb on + strand, within Pf6N2E2_4060 at 4737.375 kb on + strand, within Pf6N2E2_4060 at 4737.375 kb on + strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.376 kb on - strand, within Pf6N2E2_4060 at 4737.614 kb on + strand, within Pf6N2E2_4060 at 4737.614 kb on + strand, within Pf6N2E2_4060 at 4737.615 kb on - strand, within Pf6N2E2_4060 at 4737.687 kb on + strand, within Pf6N2E2_4060 at 4737.688 kb on - strand, within Pf6N2E2_4060 at 4737.688 kb on - strand, within Pf6N2E2_4060 at 4737.688 kb on - strand, within Pf6N2E2_4060 at 4737.789 kb on + strand, within Pf6N2E2_4060 at 4737.789 kb on + strand, within Pf6N2E2_4060 at 4737.789 kb on + strand, within Pf6N2E2_4060 at 4737.789 kb on + strand, within Pf6N2E2_4060 at 4737.790 kb on - strand, within Pf6N2E2_4060 at 4737.790 kb on - strand, within Pf6N2E2_4060 at 4737.790 kb on - strand, within Pf6N2E2_4060 at 4737.888 kb on + strand, within Pf6N2E2_4060 at 4737.889 kb on - strand, within Pf6N2E2_4060 at 4738.065 kb on + strand, within Pf6N2E2_4060 at 4738.144 kb on - strand at 4738.144 kb on - strand at 4738.359 kb on + strand, within Pf6N2E2_4061 at 4738.360 kb on - strand, within Pf6N2E2_4061 at 4738.378 kb on + strand, within Pf6N2E2_4061 at 4738.378 kb on + strand, within Pf6N2E2_4061 at 4738.378 kb on + strand, within Pf6N2E2_4061 at 4738.378 kb on + strand, within Pf6N2E2_4061 at 4738.379 kb on - strand, within Pf6N2E2_4061 at 4738.379 kb on - strand, within Pf6N2E2_4061 at 4738.379 kb on - strand, within Pf6N2E2_4061 at 4738.379 kb on - strand, within Pf6N2E2_4061 at 4738.379 kb on - strand, within Pf6N2E2_4061 at 4738.402 kb on + strand at 4738.402 kb on + strand at 4738.403 kb on - strand at 4738.403 kb on - strand at 4738.403 kb on - strand at 4738.433 kb on + strand, within Pf6N2E2_4062 at 4738.433 kb on + strand, within Pf6N2E2_4062 at 4738.433 kb on + strand, within Pf6N2E2_4062 at 4738.433 kb on + strand, within Pf6N2E2_4062 at 4738.433 kb on + strand, within Pf6N2E2_4062 at 4738.434 kb on - strand, within Pf6N2E2_4062 at 4738.434 kb on - strand, within Pf6N2E2_4062 at 4738.551 kb on + strand at 4738.551 kb on + strand at 4738.552 kb on - strand at 4738.552 kb on - strand at 4738.552 kb on - strand at 4738.597 kb on + strand at 4738.835 kb on + strand, within Pf6N2E2_4063 at 4738.835 kb on + strand, within Pf6N2E2_4063 at 4738.835 kb on + strand, within Pf6N2E2_4063 at 4738.835 kb on + strand, within Pf6N2E2_4063 at 4738.836 kb on - strand, within Pf6N2E2_4063 at 4738.836 kb on - strand, within Pf6N2E2_4063 at 4738.836 kb on - strand, within Pf6N2E2_4063 at 4738.953 kb on + strand, within Pf6N2E2_4063 at 4739.088 kb on + strand, within Pf6N2E2_4063 at 4739.089 kb on - strand, within Pf6N2E2_4063 at 4739.089 kb on - strand, within Pf6N2E2_4063 at 4739.089 kb on - strand, within Pf6N2E2_4063 at 4739.135 kb on + strand at 4739.155 kb on + strand at 4739.155 kb on + strand at 4739.155 kb on + strand at 4739.155 kb on + strand at 4739.155 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 4,737,258 + Pf6N2E2_4060 0.19 +1.2 4,737,330 + Pf6N2E2_4060 0.25 -0.3 4,737,330 + Pf6N2E2_4060 0.25 -0.5 4,737,330 + Pf6N2E2_4060 0.25 -2.0 4,737,330 + Pf6N2E2_4060 0.25 +0.3 4,737,330 + Pf6N2E2_4060 0.25 +0.5 4,737,330 + Pf6N2E2_4060 0.25 +0.5 4,737,330 + Pf6N2E2_4060 0.25 +0.6 4,737,330 + Pf6N2E2_4060 0.25 +0.8 4,737,330 + Pf6N2E2_4060 0.25 +0.5 4,737,330 + Pf6N2E2_4060 0.25 +0.1 4,737,330 + Pf6N2E2_4060 0.25 +1.3 4,737,331 - Pf6N2E2_4060 0.25 +0.3 4,737,331 - Pf6N2E2_4060 0.25 +0.0 4,737,331 - Pf6N2E2_4060 0.25 -1.2 4,737,331 - Pf6N2E2_4060 0.25 -0.5 4,737,331 - Pf6N2E2_4060 0.25 -0.3 4,737,331 - Pf6N2E2_4060 0.25 +1.1 4,737,331 - Pf6N2E2_4060 0.25 +0.2 4,737,331 - Pf6N2E2_4060 0.25 -0.8 4,737,331 - Pf6N2E2_4060 0.25 -1.2 4,737,331 - Pf6N2E2_4060 0.25 +0.3 4,737,331 - Pf6N2E2_4060 0.25 +1.8 4,737,331 - Pf6N2E2_4060 0.25 +0.1 4,737,331 - Pf6N2E2_4060 0.25 -0.2 4,737,375 + Pf6N2E2_4060 0.29 -0.5 4,737,375 + Pf6N2E2_4060 0.29 +0.9 4,737,375 + Pf6N2E2_4060 0.29 +3.2 4,737,376 - Pf6N2E2_4060 0.29 -1.6 4,737,376 - Pf6N2E2_4060 0.29 -0.6 4,737,376 - Pf6N2E2_4060 0.29 -0.2 4,737,376 - Pf6N2E2_4060 0.29 +0.6 4,737,376 - Pf6N2E2_4060 0.29 -0.8 4,737,376 - Pf6N2E2_4060 0.29 +1.0 4,737,376 - Pf6N2E2_4060 0.29 -0.4 4,737,614 + Pf6N2E2_4060 0.50 -1.3 4,737,614 + Pf6N2E2_4060 0.50 -0.5 4,737,615 - Pf6N2E2_4060 0.50 -1.3 4,737,687 + Pf6N2E2_4060 0.56 +0.5 4,737,688 - Pf6N2E2_4060 0.56 +0.7 4,737,688 - Pf6N2E2_4060 0.56 +0.5 4,737,688 - Pf6N2E2_4060 0.56 +1.0 4,737,789 + Pf6N2E2_4060 0.65 -0.2 4,737,789 + Pf6N2E2_4060 0.65 +0.4 4,737,789 + Pf6N2E2_4060 0.65 +0.2 4,737,789 + Pf6N2E2_4060 0.65 -1.1 4,737,790 - Pf6N2E2_4060 0.65 +0.6 4,737,790 - Pf6N2E2_4060 0.65 +0.3 4,737,790 - Pf6N2E2_4060 0.65 +0.4 4,737,888 + Pf6N2E2_4060 0.73 -0.4 4,737,889 - Pf6N2E2_4060 0.73 +0.4 4,738,065 + Pf6N2E2_4060 0.88 -0.1 4,738,144 - +0.5 4,738,144 - +0.2 4,738,359 + Pf6N2E2_4061 0.77 +0.8 4,738,360 - Pf6N2E2_4061 0.78 -0.6 4,738,378 + Pf6N2E2_4061 0.89 -0.6 4,738,378 + Pf6N2E2_4061 0.89 -0.1 4,738,378 + Pf6N2E2_4061 0.89 -0.4 4,738,378 + Pf6N2E2_4061 0.89 -0.6 4,738,379 - Pf6N2E2_4061 0.89 -0.5 4,738,379 - Pf6N2E2_4061 0.89 -0.0 4,738,379 - Pf6N2E2_4061 0.89 -0.7 4,738,379 - Pf6N2E2_4061 0.89 -1.4 4,738,379 - Pf6N2E2_4061 0.89 +1.0 4,738,402 + +0.1 4,738,402 + +0.1 4,738,403 - +0.5 4,738,403 - +0.6 4,738,403 - -0.2 4,738,433 + Pf6N2E2_4062 0.26 -1.3 4,738,433 + Pf6N2E2_4062 0.26 +1.0 4,738,433 + Pf6N2E2_4062 0.26 +0.7 4,738,433 + Pf6N2E2_4062 0.26 +0.0 4,738,433 + Pf6N2E2_4062 0.26 +0.6 4,738,434 - Pf6N2E2_4062 0.26 -0.8 4,738,434 - Pf6N2E2_4062 0.26 -2.0 4,738,551 + -0.2 4,738,551 + +0.1 4,738,552 - -0.0 4,738,552 - -2.5 4,738,552 - +0.6 4,738,597 + +1.0 4,738,835 + Pf6N2E2_4063 0.48 +0.6 4,738,835 + Pf6N2E2_4063 0.48 -0.1 4,738,835 + Pf6N2E2_4063 0.48 -0.1 4,738,835 + Pf6N2E2_4063 0.48 +0.4 4,738,836 - Pf6N2E2_4063 0.48 -0.2 4,738,836 - Pf6N2E2_4063 0.48 +0.8 4,738,836 - Pf6N2E2_4063 0.48 -0.4 4,738,953 + Pf6N2E2_4063 0.67 -0.5 4,739,088 + Pf6N2E2_4063 0.89 -0.7 4,739,089 - Pf6N2E2_4063 0.89 -2.3 4,739,089 - Pf6N2E2_4063 0.89 -1.6 4,739,089 - Pf6N2E2_4063 0.89 +0.3 4,739,135 + +0.6 4,739,155 + -0.9 4,739,155 + -0.7 4,739,155 + +0.8 4,739,155 + +0.3 4,739,155 + -1.3
Or see this region's nucleotide sequence