Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_344

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_343 and Pf6N2E2_344 are separated by 4 nucleotidesPf6N2E2_344 and Pf6N2E2_345 are separated by 153 nucleotides Pf6N2E2_343 - VacJ-like lipoprotein precursor, at 410,495 to 411,226 _343 Pf6N2E2_344 - hypothetical protein, at 411,231 to 411,455 _344 Pf6N2E2_345 - Manganese transport protein MntH, at 411,609 to 412,910 _345 Position (kb) 411 412Strain fitness (log2 ratio) -2 -1 0 1 2at 410.404 kb on - strandat 410.437 kb on + strandat 410.490 kb on + strandat 410.490 kb on + strandat 410.491 kb on - strandat 410.491 kb on - strandat 410.491 kb on - strandat 410.561 kb on + strandat 410.561 kb on + strandat 410.561 kb on + strandat 410.562 kb on - strandat 410.562 kb on - strandat 410.630 kb on + strand, within Pf6N2E2_343at 410.630 kb on + strand, within Pf6N2E2_343at 410.630 kb on + strand, within Pf6N2E2_343at 410.630 kb on + strand, within Pf6N2E2_343at 410.630 kb on + strand, within Pf6N2E2_343at 410.630 kb on + strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.631 kb on - strand, within Pf6N2E2_343at 410.734 kb on - strand, within Pf6N2E2_343at 410.745 kb on + strand, within Pf6N2E2_343at 410.745 kb on + strand, within Pf6N2E2_343at 410.745 kb on + strand, within Pf6N2E2_343at 410.745 kb on + strand, within Pf6N2E2_343at 410.746 kb on - strand, within Pf6N2E2_343at 410.746 kb on - strand, within Pf6N2E2_343at 410.746 kb on - strand, within Pf6N2E2_343at 410.858 kb on - strand, within Pf6N2E2_343at 411.104 kb on + strand, within Pf6N2E2_343at 411.105 kb on - strandat 411.105 kb on - strand, within Pf6N2E2_343at 411.105 kb on - strand, within Pf6N2E2_343at 411.107 kb on + strand, within Pf6N2E2_343at 411.340 kb on - strand, within Pf6N2E2_344at 411.340 kb on - strand, within Pf6N2E2_344at 411.351 kb on + strand, within Pf6N2E2_344at 411.352 kb on - strand, within Pf6N2E2_344at 411.352 kb on - strand, within Pf6N2E2_344at 411.483 kb on + strandat 411.484 kb on - strandat 411.518 kb on + strandat 411.518 kb on + strandat 411.762 kb on + strand, within Pf6N2E2_345at 411.810 kb on + strand, within Pf6N2E2_345at 411.810 kb on + strand, within Pf6N2E2_345at 411.879 kb on + strand, within Pf6N2E2_345at 411.879 kb on + strand, within Pf6N2E2_345at 411.879 kb on + strand, within Pf6N2E2_345at 411.880 kb on - strand, within Pf6N2E2_345at 411.921 kb on + strand, within Pf6N2E2_345at 411.921 kb on + strand, within Pf6N2E2_345at 411.921 kb on + strand, within Pf6N2E2_345at 411.954 kb on + strand, within Pf6N2E2_345at 411.954 kb on + strand, within Pf6N2E2_345at 411.954 kb on + strand, within Pf6N2E2_345at 411.954 kb on + strand, within Pf6N2E2_345at 411.954 kb on + strand, within Pf6N2E2_345at 411.955 kb on - strand, within Pf6N2E2_345at 411.955 kb on - strand, within Pf6N2E2_345at 411.955 kb on - strand, within Pf6N2E2_345at 411.955 kb on - strand, within Pf6N2E2_345at 412.065 kb on - strand, within Pf6N2E2_345at 412.068 kb on + strand, within Pf6N2E2_345at 412.068 kb on + strand, within Pf6N2E2_345at 412.068 kb on + strand, within Pf6N2E2_345at 412.284 kb on + strand, within Pf6N2E2_345at 412.284 kb on + strand, within Pf6N2E2_345at 412.328 kb on + strand, within Pf6N2E2_345at 412.329 kb on - strand, within Pf6N2E2_345

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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410,404 - +0.2
410,437 + +0.5
410,490 + -2.1
410,490 + -0.8
410,491 - +0.1
410,491 - -1.3
410,491 - -0.1
410,561 + -1.4
410,561 + +0.1
410,561 + +1.0
410,562 - -2.6
410,562 - +0.6
410,630 + Pf6N2E2_343 0.18 +1.0
410,630 + Pf6N2E2_343 0.18 +0.2
410,630 + Pf6N2E2_343 0.18 +0.3
410,630 + Pf6N2E2_343 0.18 +0.3
410,630 + Pf6N2E2_343 0.18 +0.6
410,630 + Pf6N2E2_343 0.18 +1.0
410,631 - Pf6N2E2_343 0.19 -0.0
410,631 - Pf6N2E2_343 0.19 +0.9
410,631 - Pf6N2E2_343 0.19 +0.8
410,631 - Pf6N2E2_343 0.19 -0.1
410,631 - Pf6N2E2_343 0.19 -0.1
410,631 - Pf6N2E2_343 0.19 -0.1
410,631 - Pf6N2E2_343 0.19 +1.0
410,734 - Pf6N2E2_343 0.33 -0.3
410,745 + Pf6N2E2_343 0.34 -0.4
410,745 + Pf6N2E2_343 0.34 +0.2
410,745 + Pf6N2E2_343 0.34 +1.1
410,745 + Pf6N2E2_343 0.34 +2.1
410,746 - Pf6N2E2_343 0.34 +1.4
410,746 - Pf6N2E2_343 0.34 -0.4
410,746 - Pf6N2E2_343 0.34 +0.9
410,858 - Pf6N2E2_343 0.50 -0.6
411,104 + Pf6N2E2_343 0.83 -0.3
411,105 - -0.1
411,105 - Pf6N2E2_343 0.83 -0.2
411,105 - Pf6N2E2_343 0.83 -1.3
411,107 + Pf6N2E2_343 0.84 +1.4
411,340 - Pf6N2E2_344 0.48 +0.9
411,340 - Pf6N2E2_344 0.48 +1.5
411,351 + Pf6N2E2_344 0.53 +0.0
411,352 - Pf6N2E2_344 0.54 +0.7
411,352 - Pf6N2E2_344 0.54 +2.5
411,483 + -0.8
411,484 - +0.4
411,518 + +0.3
411,518 + +0.1
411,762 + Pf6N2E2_345 0.12 +0.5
411,810 + Pf6N2E2_345 0.15 +0.8
411,810 + Pf6N2E2_345 0.15 -1.0
411,879 + Pf6N2E2_345 0.21 -0.3
411,879 + Pf6N2E2_345 0.21 -0.6
411,879 + Pf6N2E2_345 0.21 +0.6
411,880 - Pf6N2E2_345 0.21 -0.1
411,921 + Pf6N2E2_345 0.24 +1.5
411,921 + Pf6N2E2_345 0.24 +1.0
411,921 + Pf6N2E2_345 0.24 -0.5
411,954 + Pf6N2E2_345 0.26 +0.0
411,954 + Pf6N2E2_345 0.26 -1.0
411,954 + Pf6N2E2_345 0.26 +0.7
411,954 + Pf6N2E2_345 0.26 +0.2
411,954 + Pf6N2E2_345 0.26 +0.5
411,955 - Pf6N2E2_345 0.27 +1.8
411,955 - Pf6N2E2_345 0.27 -0.6
411,955 - Pf6N2E2_345 0.27 -1.5
411,955 - Pf6N2E2_345 0.27 +1.6
412,065 - Pf6N2E2_345 0.35 -0.1
412,068 + Pf6N2E2_345 0.35 -0.0
412,068 + Pf6N2E2_345 0.35 +1.3
412,068 + Pf6N2E2_345 0.35 +0.0
412,284 + Pf6N2E2_345 0.52 -1.2
412,284 + Pf6N2E2_345 0.52 -1.9
412,328 + Pf6N2E2_345 0.55 +0.3
412,329 - Pf6N2E2_345 0.55 +0.9

Or see this region's nucleotide sequence