Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_334 and Pf6N2E2_335 are separated by 100 nucleotides Pf6N2E2_335 and Pf6N2E2_336 overlap by 4 nucleotides Pf6N2E2_336 and Pf6N2E2_337 are separated by 173 nucleotides Pf6N2E2_337 and Pf6N2E2_338 are separated by 147 nucleotides
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Pf6N2E2_335 - Aldo-keto reductase, at 402,771 to 403,634
_335
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Pf6N2E2_338 - Transcriptional regulator, AraC family, at 405,007 to 405,510
_338
Position (kb) 403
404
405 Strain fitness (log2 ratio) -2
-1
0
1
2 at 402.686 kb on - strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.957 kb on + strand, within Pf6N2E2_335 at 403.006 kb on - strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.633 kb on - strand at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.865 kb on + strand, within Pf6N2E2_336 at 403.866 kb on - strand, within Pf6N2E2_336 at 403.866 kb on - strand at 403.866 kb on - strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 403.938 kb on + strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.039 kb on - strand, within Pf6N2E2_336 at 404.116 kb on - strand, within Pf6N2E2_336 at 404.467 kb on + strand at 404.467 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand at 404.669 kb on - strand, within Pf6N2E2_337 at 404.875 kb on + strand at 404.875 kb on + strand at 404.876 kb on - strand at 405.098 kb on + strand, within Pf6N2E2_338 at 405.098 kb on + strand, within Pf6N2E2_338
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 402,686 - -0.4 402,733 + -0.1 402,733 + +1.8 402,733 + +2.0 402,733 + -0.7 402,733 + +1.7 402,734 - -0.5 402,734 - +0.6 402,734 - -0.0 402,734 - -0.6 402,734 - -0.0 402,734 - -0.6 402,957 + Pf6N2E2_335 0.22 +0.7 403,006 - Pf6N2E2_335 0.27 -0.3 403,332 + Pf6N2E2_335 0.65 -2.0 403,332 + Pf6N2E2_335 0.65 +0.1 403,395 + Pf6N2E2_335 0.72 -0.4 403,395 + Pf6N2E2_335 0.72 +0.6 403,396 - Pf6N2E2_335 0.72 +0.7 403,396 - Pf6N2E2_335 0.72 +0.1 403,396 - Pf6N2E2_335 0.72 +0.2 403,633 - +0.0 403,865 + Pf6N2E2_336 0.30 -0.3 403,865 + Pf6N2E2_336 0.30 +1.8 403,865 + Pf6N2E2_336 0.30 +0.5 403,865 + Pf6N2E2_336 0.30 +1.9 403,866 - Pf6N2E2_336 0.30 +0.4 403,866 - -0.1 403,866 - Pf6N2E2_336 0.30 +1.5 403,938 + Pf6N2E2_336 0.39 +0.0 403,938 + Pf6N2E2_336 0.39 +0.5 403,938 + Pf6N2E2_336 0.39 -0.9 404,039 - Pf6N2E2_336 0.52 +0.3 404,039 - Pf6N2E2_336 0.52 +1.0 404,039 - Pf6N2E2_336 0.52 +2.3 404,116 - Pf6N2E2_336 0.62 -0.5 404,467 + +1.1 404,467 + -1.9 404,536 + -0.1 404,536 + +0.7 404,536 + +0.9 404,537 - +0.6 404,669 - Pf6N2E2_337 0.30 +0.0 404,875 + +0.4 404,875 + +0.4 404,876 - +0.3 405,098 + Pf6N2E2_338 0.18 -1.1 405,098 + Pf6N2E2_338 0.18 +0.9
Or see this region's nucleotide sequence