Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3000 and Pf6N2E2_3001 are separated by 11 nucleotides Pf6N2E2_3001 and Pf6N2E2_3002 overlap by 13 nucleotides Pf6N2E2_3002 and Pf6N2E2_3003 overlap by 11 nucleotides Pf6N2E2_3003 and Pf6N2E2_3004 overlap by 4 nucleotides
Pf6N2E2_3000 - Prophage tail fiber protein, at 3,490,214 to 3,491,377
_3000
Pf6N2E2_3001 - FIG121501: Prophage tail protein, at 3,491,389 to 3,491,988
_3001
Pf6N2E2_3002 - Phage FluMu protein gp47, at 3,491,976 to 3,493,016
_3002
Pf6N2E2_3003 - Bacteriophage protein GP46, at 3,493,006 to 3,493,407
_3003
Pf6N2E2_3004 - Prophage baseplate assembly protein V, at 3,493,404 to 3,493,913
_3004
Position (kb)
3491
3492
3493
3494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3490.976 kb on - strand, within Pf6N2E2_3000 at 3490.976 kb on - strand, within Pf6N2E2_3000 at 3491.000 kb on + strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.001 kb on - strand, within Pf6N2E2_3000 at 3491.025 kb on + strand, within Pf6N2E2_3000 at 3491.025 kb on + strand, within Pf6N2E2_3000 at 3491.025 kb on + strand, within Pf6N2E2_3000 at 3491.025 kb on + strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.026 kb on - strand, within Pf6N2E2_3000 at 3491.384 kb on - strand at 3491.397 kb on - strand at 3491.397 kb on - strand at 3491.397 kb on - strand at 3491.397 kb on - strand at 3491.397 kb on - strand at 3491.397 kb on - strand at 3491.416 kb on - strand at 3491.666 kb on + strand, within Pf6N2E2_3001 at 3491.666 kb on + strand, within Pf6N2E2_3001 at 3491.666 kb on + strand, within Pf6N2E2_3001 at 3491.667 kb on - strand, within Pf6N2E2_3001 at 3491.667 kb on - strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.669 kb on + strand, within Pf6N2E2_3001 at 3491.670 kb on - strand, within Pf6N2E2_3001 at 3491.670 kb on - strand, within Pf6N2E2_3001 at 3491.732 kb on + strand, within Pf6N2E2_3001 at 3491.732 kb on + strand, within Pf6N2E2_3001 at 3491.733 kb on - strand, within Pf6N2E2_3001 at 3491.804 kb on - strand, within Pf6N2E2_3001 at 3491.804 kb on - strand, within Pf6N2E2_3001 at 3491.804 kb on - strand, within Pf6N2E2_3001 at 3491.961 kb on - strand at 3491.961 kb on - strand at 3491.977 kb on + strand at 3491.978 kb on - strand at 3491.978 kb on - strand at 3492.280 kb on + strand, within Pf6N2E2_3002 at 3492.281 kb on - strand, within Pf6N2E2_3002 at 3492.281 kb on - strand, within Pf6N2E2_3002 at 3492.373 kb on + strand, within Pf6N2E2_3002 at 3492.430 kb on + strand, within Pf6N2E2_3002 at 3492.431 kb on - strand, within Pf6N2E2_3002 at 3492.552 kb on - strand, within Pf6N2E2_3002 at 3492.552 kb on - strand, within Pf6N2E2_3002 at 3492.666 kb on - strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.886 kb on + strand, within Pf6N2E2_3002 at 3492.887 kb on - strand, within Pf6N2E2_3002 at 3492.887 kb on - strand, within Pf6N2E2_3002 at 3492.887 kb on - strand, within Pf6N2E2_3002 at 3492.887 kb on - strand, within Pf6N2E2_3002 at 3492.887 kb on - strand, within Pf6N2E2_3002 at 3493.016 kb on + strand at 3493.016 kb on + strand at 3493.017 kb on - strand at 3493.017 kb on - strand at 3493.017 kb on - strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.032 kb on + strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.033 kb on - strand at 3493.182 kb on - strand, within Pf6N2E2_3003 at 3493.182 kb on - strand, within Pf6N2E2_3003 at 3493.182 kb on - strand, within Pf6N2E2_3003 at 3493.449 kb on + strand at 3493.449 kb on + strand at 3493.450 kb on - strand at 3493.642 kb on + strand, within Pf6N2E2_3004 at 3493.642 kb on + strand, within Pf6N2E2_3004 at 3493.642 kb on + strand, within Pf6N2E2_3004 at 3493.905 kb on + strand at 3493.905 kb on + strand at 3493.967 kb on + strand at 3493.967 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 3,490,976 - Pf6N2E2_3000 0.65 +0.1 3,490,976 - Pf6N2E2_3000 0.65 -0.7 3,491,000 + Pf6N2E2_3000 0.68 -0.1 3,491,001 - Pf6N2E2_3000 0.68 -0.7 3,491,001 - Pf6N2E2_3000 0.68 +0.5 3,491,001 - Pf6N2E2_3000 0.68 -0.1 3,491,001 - Pf6N2E2_3000 0.68 -0.4 3,491,001 - Pf6N2E2_3000 0.68 +1.2 3,491,001 - Pf6N2E2_3000 0.68 +0.8 3,491,001 - Pf6N2E2_3000 0.68 -0.2 3,491,025 + Pf6N2E2_3000 0.70 -1.4 3,491,025 + Pf6N2E2_3000 0.70 +0.7 3,491,025 + Pf6N2E2_3000 0.70 +0.6 3,491,025 + Pf6N2E2_3000 0.70 -0.3 3,491,026 - Pf6N2E2_3000 0.70 -0.6 3,491,026 - Pf6N2E2_3000 0.70 -1.5 3,491,026 - Pf6N2E2_3000 0.70 +0.2 3,491,026 - Pf6N2E2_3000 0.70 -0.1 3,491,026 - Pf6N2E2_3000 0.70 -0.5 3,491,026 - Pf6N2E2_3000 0.70 +0.8 3,491,384 - +0.5 3,491,397 - +1.2 3,491,397 - -1.7 3,491,397 - -0.8 3,491,397 - -0.2 3,491,397 - +0.0 3,491,397 - +0.1 3,491,416 - -0.4 3,491,666 + Pf6N2E2_3001 0.46 -0.0 3,491,666 + Pf6N2E2_3001 0.46 -0.6 3,491,666 + Pf6N2E2_3001 0.46 +0.2 3,491,667 - Pf6N2E2_3001 0.46 -0.3 3,491,667 - Pf6N2E2_3001 0.46 -1.1 3,491,669 + Pf6N2E2_3001 0.47 +0.1 3,491,669 + Pf6N2E2_3001 0.47 +0.5 3,491,669 + Pf6N2E2_3001 0.47 -0.0 3,491,669 + Pf6N2E2_3001 0.47 +0.7 3,491,669 + Pf6N2E2_3001 0.47 +1.8 3,491,669 + Pf6N2E2_3001 0.47 -1.1 3,491,670 - Pf6N2E2_3001 0.47 -0.7 3,491,670 - Pf6N2E2_3001 0.47 +2.2 3,491,732 + Pf6N2E2_3001 0.57 -0.4 3,491,732 + Pf6N2E2_3001 0.57 +0.8 3,491,733 - Pf6N2E2_3001 0.57 +1.9 3,491,804 - Pf6N2E2_3001 0.69 +0.7 3,491,804 - Pf6N2E2_3001 0.69 +0.7 3,491,804 - Pf6N2E2_3001 0.69 +0.1 3,491,961 - -0.5 3,491,961 - +0.4 3,491,977 + +0.8 3,491,978 - +0.5 3,491,978 - -0.5 3,492,280 + Pf6N2E2_3002 0.29 +0.7 3,492,281 - Pf6N2E2_3002 0.29 -0.8 3,492,281 - Pf6N2E2_3002 0.29 +0.2 3,492,373 + Pf6N2E2_3002 0.38 +1.4 3,492,430 + Pf6N2E2_3002 0.44 +0.4 3,492,431 - Pf6N2E2_3002 0.44 -0.0 3,492,552 - Pf6N2E2_3002 0.55 +1.1 3,492,552 - Pf6N2E2_3002 0.55 +1.9 3,492,666 - Pf6N2E2_3002 0.66 -0.0 3,492,886 + Pf6N2E2_3002 0.87 +0.2 3,492,886 + Pf6N2E2_3002 0.87 +0.6 3,492,886 + Pf6N2E2_3002 0.87 -0.3 3,492,886 + Pf6N2E2_3002 0.87 -0.1 3,492,886 + Pf6N2E2_3002 0.87 +0.0 3,492,886 + Pf6N2E2_3002 0.87 +1.1 3,492,886 + Pf6N2E2_3002 0.87 +0.2 3,492,887 - Pf6N2E2_3002 0.88 +1.4 3,492,887 - Pf6N2E2_3002 0.88 -0.1 3,492,887 - Pf6N2E2_3002 0.88 +1.0 3,492,887 - Pf6N2E2_3002 0.88 +1.2 3,492,887 - Pf6N2E2_3002 0.88 +0.2 3,493,016 + +1.3 3,493,016 + -0.5 3,493,017 - +1.0 3,493,017 - +1.2 3,493,017 - +0.6 3,493,032 + +0.1 3,493,032 + +0.1 3,493,032 + -0.5 3,493,032 + +0.6 3,493,032 + +0.6 3,493,032 + +0.6 3,493,032 + -0.6 3,493,033 - +1.3 3,493,033 - +0.2 3,493,033 - -2.8 3,493,033 - -1.6 3,493,033 - +0.8 3,493,033 - -0.4 3,493,033 - +0.2 3,493,182 - Pf6N2E2_3003 0.44 -0.0 3,493,182 - Pf6N2E2_3003 0.44 +0.3 3,493,182 - Pf6N2E2_3003 0.44 -0.2 3,493,449 + +0.1 3,493,449 + +0.6 3,493,450 - +0.2 3,493,642 + Pf6N2E2_3004 0.47 -1.4 3,493,642 + Pf6N2E2_3004 0.47 +0.1 3,493,642 + Pf6N2E2_3004 0.47 -0.5 3,493,905 + +0.1 3,493,905 + -0.3 3,493,967 + +0.3 3,493,967 + -0.7
Or see this region's nucleotide sequence