Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_249 and Pf6N2E2_250 are separated by 262 nucleotides Pf6N2E2_250 and Pf6N2E2_251 are separated by 184 nucleotides
Pf6N2E2_249 - Pirin, at 310,575 to 311,297
_249
Pf6N2E2_250 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 311,560 to 312,495
_250
Pf6N2E2_251 - transcriptional regulator, at 312,680 to 314,167
_251
Position (kb) 311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 310.699 kb on - strand, within Pf6N2E2_249 at 310.738 kb on + strand, within Pf6N2E2_249 at 310.738 kb on + strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.739 kb on - strand, within Pf6N2E2_249 at 310.744 kb on + strand, within Pf6N2E2_249 at 310.745 kb on - strand, within Pf6N2E2_249 at 310.745 kb on - strand, within Pf6N2E2_249 at 310.802 kb on - strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.098 kb on + strand, within Pf6N2E2_249 at 311.099 kb on - strand, within Pf6N2E2_249 at 311.460 kb on + strand at 311.460 kb on + strand at 311.460 kb on + strand at 311.461 kb on - strand at 311.461 kb on - strand at 311.512 kb on + strand at 311.512 kb on + strand at 311.513 kb on - strand at 311.513 kb on - strand at 311.513 kb on - strand at 311.513 kb on - strand at 311.513 kb on - strand at 311.514 kb on + strand at 311.632 kb on + strand at 311.753 kb on - strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.788 kb on + strand, within Pf6N2E2_250 at 311.933 kb on - strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 311.987 kb on + strand, within Pf6N2E2_250 at 312.578 kb on + strand at 312.619 kb on - strand at 312.653 kb on + strand at 312.839 kb on + strand, within Pf6N2E2_251 at 312.962 kb on + strand, within Pf6N2E2_251 at 312.962 kb on + strand at 312.962 kb on + strand, within Pf6N2E2_251 at 312.963 kb on - strand, within Pf6N2E2_251 at 312.989 kb on + strand, within Pf6N2E2_251
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 310,699 - Pf6N2E2_249 0.17 -0.5 310,738 + Pf6N2E2_249 0.23 +1.0 310,738 + Pf6N2E2_249 0.23 +0.6 310,739 - Pf6N2E2_249 0.23 +2.2 310,739 - Pf6N2E2_249 0.23 +0.5 310,739 - Pf6N2E2_249 0.23 -2.2 310,739 - Pf6N2E2_249 0.23 -0.0 310,744 + Pf6N2E2_249 0.23 -0.5 310,745 - Pf6N2E2_249 0.24 -3.2 310,745 - Pf6N2E2_249 0.24 +0.5 310,802 - Pf6N2E2_249 0.31 -0.1 311,098 + Pf6N2E2_249 0.72 +0.5 311,098 + Pf6N2E2_249 0.72 -0.1 311,098 + Pf6N2E2_249 0.72 -0.7 311,099 - Pf6N2E2_249 0.72 +0.6 311,460 + -0.9 311,460 + -0.3 311,460 + +0.5 311,461 - -1.0 311,461 - -0.3 311,512 + +0.2 311,512 + +0.5 311,513 - -0.7 311,513 - +0.5 311,513 - -0.4 311,513 - -0.0 311,513 - +0.6 311,514 + -0.0 311,632 + +0.2 311,753 - Pf6N2E2_250 0.21 +0.5 311,788 + Pf6N2E2_250 0.24 +2.0 311,788 + Pf6N2E2_250 0.24 -0.0 311,788 + Pf6N2E2_250 0.24 +0.2 311,788 + Pf6N2E2_250 0.24 -0.7 311,933 - Pf6N2E2_250 0.40 +1.0 311,987 + Pf6N2E2_250 0.46 +1.7 311,987 + Pf6N2E2_250 0.46 +0.7 311,987 + Pf6N2E2_250 0.46 +0.5 311,987 + Pf6N2E2_250 0.46 -2.0 312,578 + -0.2 312,619 - -0.6 312,653 + +0.5 312,839 + Pf6N2E2_251 0.11 +0.0 312,962 + Pf6N2E2_251 0.19 -0.2 312,962 + -0.1 312,962 + Pf6N2E2_251 0.19 -0.9 312,963 - Pf6N2E2_251 0.19 +1.2 312,989 + Pf6N2E2_251 0.21 -1.3
Or see this region's nucleotide sequence