Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_200

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_196 and Pf6N2E2_197 overlap by 4 nucleotidesPf6N2E2_197 and Pf6N2E2_198 are separated by 106 nucleotidesPf6N2E2_198 and Pf6N2E2_199 are separated by 9 nucleotidesPf6N2E2_199 and Pf6N2E2_200 are separated by 60 nucleotidesPf6N2E2_200 and Pf6N2E2_201 overlap by 10 nucleotides Pf6N2E2_196 - hypothetical protein, at 249,721 to 249,948 _196 Pf6N2E2_197 - Death on curing protein, Doc toxin, at 249,945 to 250,262 _197 Pf6N2E2_198 - hypothetical protein, at 250,369 to 250,527 _198 Pf6N2E2_199 - hypothetical protein, at 250,537 to 250,800 _199 Pf6N2E2_200 - Possible glycosyltransferase, at 250,861 to 251,967 _200 Pf6N2E2_201 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 251,958 to 253,067 _201 Position (kb) 250 251 252Strain fitness (log2 ratio) -2 -1 0 1at 249.991 kb on + strand, within Pf6N2E2_197at 249.991 kb on + strand, within Pf6N2E2_197at 249.991 kb on + strand, within Pf6N2E2_197at 249.992 kb on - strand, within Pf6N2E2_197at 250.008 kb on + strand, within Pf6N2E2_197at 250.009 kb on - strand, within Pf6N2E2_197at 250.009 kb on - strand, within Pf6N2E2_197at 250.012 kb on - strand, within Pf6N2E2_197at 250.012 kb on - strand, within Pf6N2E2_197at 250.071 kb on + strand, within Pf6N2E2_197at 250.071 kb on + strand, within Pf6N2E2_197at 250.071 kb on + strand, within Pf6N2E2_197at 250.071 kb on + strand, within Pf6N2E2_197at 250.072 kb on - strand, within Pf6N2E2_197at 250.072 kb on - strand, within Pf6N2E2_197at 250.072 kb on - strand, within Pf6N2E2_197at 250.073 kb on + strand, within Pf6N2E2_197at 250.073 kb on + strand, within Pf6N2E2_197at 250.073 kb on + strand, within Pf6N2E2_197at 250.073 kb on + strand, within Pf6N2E2_197at 250.073 kb on + strand, within Pf6N2E2_197at 250.074 kb on - strand, within Pf6N2E2_197at 250.074 kb on - strand, within Pf6N2E2_197at 250.074 kb on - strand, within Pf6N2E2_197at 250.074 kb on - strand, within Pf6N2E2_197at 250.074 kb on - strand, within Pf6N2E2_197at 250.103 kb on + strand, within Pf6N2E2_197at 250.104 kb on - strand, within Pf6N2E2_197at 250.114 kb on - strand, within Pf6N2E2_197at 250.171 kb on - strand, within Pf6N2E2_197at 250.182 kb on + strand, within Pf6N2E2_197at 250.182 kb on + strand, within Pf6N2E2_197at 250.182 kb on + strand, within Pf6N2E2_197at 250.182 kb on + strand, within Pf6N2E2_197at 250.211 kb on - strand, within Pf6N2E2_197at 250.429 kb on + strand, within Pf6N2E2_198at 250.430 kb on - strand, within Pf6N2E2_198at 250.430 kb on - strand, within Pf6N2E2_198at 250.430 kb on - strand, within Pf6N2E2_198at 250.455 kb on + strand, within Pf6N2E2_198at 250.455 kb on + strand, within Pf6N2E2_198at 250.455 kb on + strand, within Pf6N2E2_198at 250.456 kb on - strand, within Pf6N2E2_198at 250.456 kb on - strand, within Pf6N2E2_198at 250.456 kb on - strand, within Pf6N2E2_198at 250.480 kb on + strand, within Pf6N2E2_198at 250.748 kb on + strand, within Pf6N2E2_199at 250.749 kb on - strand, within Pf6N2E2_199at 250.749 kb on - strand, within Pf6N2E2_199at 250.749 kb on - strand, within Pf6N2E2_199at 250.777 kb on + strandat 250.778 kb on - strandat 250.787 kb on + strandat 250.787 kb on + strandat 250.809 kb on + strandat 250.810 kb on - strandat 250.843 kb on + strandat 251.087 kb on + strand, within Pf6N2E2_200at 251.088 kb on - strand, within Pf6N2E2_200at 251.222 kb on - strand, within Pf6N2E2_200at 251.222 kb on - strand, within Pf6N2E2_200at 251.422 kb on + strand, within Pf6N2E2_200at 251.423 kb on - strand, within Pf6N2E2_200at 251.476 kb on + strand, within Pf6N2E2_200at 251.476 kb on + strand, within Pf6N2E2_200at 251.477 kb on - strand, within Pf6N2E2_200at 251.544 kb on - strand, within Pf6N2E2_200at 251.574 kb on - strand, within Pf6N2E2_200at 251.870 kb on - strandat 251.954 kb on + strandat 251.954 kb on + strandat 251.954 kb on + strandat 251.955 kb on - strandat 251.955 kb on - strandat 251.955 kb on - strandat 251.977 kb on + strandat 251.977 kb on + strandat 252.056 kb on - strandat 252.241 kb on + strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.688 kb on - strand, within Pf6N2E2_201at 252.688 kb on - strand, within Pf6N2E2_201

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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249,991 + Pf6N2E2_197 0.14 +0.9
249,991 + Pf6N2E2_197 0.14 +0.3
249,991 + Pf6N2E2_197 0.14 -0.3
249,992 - Pf6N2E2_197 0.15 +0.6
250,008 + Pf6N2E2_197 0.20 +0.4
250,009 - Pf6N2E2_197 0.20 +0.6
250,009 - Pf6N2E2_197 0.20 -0.5
250,012 - Pf6N2E2_197 0.21 -1.8
250,012 - Pf6N2E2_197 0.21 -0.4
250,071 + Pf6N2E2_197 0.40 +0.7
250,071 + Pf6N2E2_197 0.40 -0.3
250,071 + Pf6N2E2_197 0.40 -0.1
250,071 + Pf6N2E2_197 0.40 -1.3
250,072 - Pf6N2E2_197 0.40 +0.4
250,072 - Pf6N2E2_197 0.40 -1.9
250,072 - Pf6N2E2_197 0.40 +0.1
250,073 + Pf6N2E2_197 0.40 +1.6
250,073 + Pf6N2E2_197 0.40 -0.1
250,073 + Pf6N2E2_197 0.40 +0.1
250,073 + Pf6N2E2_197 0.40 -0.0
250,073 + Pf6N2E2_197 0.40 +0.2
250,074 - Pf6N2E2_197 0.41 +0.2
250,074 - Pf6N2E2_197 0.41 +0.7
250,074 - Pf6N2E2_197 0.41 +0.2
250,074 - Pf6N2E2_197 0.41 -0.7
250,074 - Pf6N2E2_197 0.41 -0.4
250,103 + Pf6N2E2_197 0.50 +0.2
250,104 - Pf6N2E2_197 0.50 -0.8
250,114 - Pf6N2E2_197 0.53 -1.3
250,171 - Pf6N2E2_197 0.71 -0.5
250,182 + Pf6N2E2_197 0.75 +0.4
250,182 + Pf6N2E2_197 0.75 +0.8
250,182 + Pf6N2E2_197 0.75 +0.7
250,182 + Pf6N2E2_197 0.75 +0.6
250,211 - Pf6N2E2_197 0.84 -1.1
250,429 + Pf6N2E2_198 0.38 -0.6
250,430 - Pf6N2E2_198 0.38 +0.2
250,430 - Pf6N2E2_198 0.38 -0.7
250,430 - Pf6N2E2_198 0.38 +0.3
250,455 + Pf6N2E2_198 0.54 +0.3
250,455 + Pf6N2E2_198 0.54 +0.3
250,455 + Pf6N2E2_198 0.54 -1.0
250,456 - Pf6N2E2_198 0.55 -0.5
250,456 - Pf6N2E2_198 0.55 -0.7
250,456 - Pf6N2E2_198 0.55 +1.3
250,480 + Pf6N2E2_198 0.70 -0.3
250,748 + Pf6N2E2_199 0.80 +0.7
250,749 - Pf6N2E2_199 0.80 +0.3
250,749 - Pf6N2E2_199 0.80 -1.1
250,749 - Pf6N2E2_199 0.80 +0.3
250,777 + -0.4
250,778 - -0.2
250,787 + -0.0
250,787 + -0.5
250,809 + +0.6
250,810 - +0.9
250,843 + -0.5
251,087 + Pf6N2E2_200 0.20 -1.4
251,088 - Pf6N2E2_200 0.21 -1.2
251,222 - Pf6N2E2_200 0.33 -1.1
251,222 - Pf6N2E2_200 0.33 -1.3
251,422 + Pf6N2E2_200 0.51 +0.7
251,423 - Pf6N2E2_200 0.51 +0.9
251,476 + Pf6N2E2_200 0.56 +1.2
251,476 + Pf6N2E2_200 0.56 +1.5
251,477 - Pf6N2E2_200 0.56 +1.7
251,544 - Pf6N2E2_200 0.62 +0.8
251,574 - Pf6N2E2_200 0.64 +0.7
251,870 - -0.0
251,954 + +0.1
251,954 + -1.0
251,954 + -0.2
251,955 - -0.6
251,955 - +0.0
251,955 - +0.9
251,977 + +0.6
251,977 + +0.1
252,056 - +1.0
252,241 + Pf6N2E2_201 0.25 +0.0
252,242 - Pf6N2E2_201 0.26 +0.4
252,242 - Pf6N2E2_201 0.26 -0.1
252,242 - Pf6N2E2_201 0.26 +0.3
252,274 + Pf6N2E2_201 0.28 +1.0
252,274 + Pf6N2E2_201 0.28 +0.1
252,274 + Pf6N2E2_201 0.28 -1.4
252,688 - Pf6N2E2_201 0.66 +0.2
252,688 - Pf6N2E2_201 0.66 -1.3

Or see this region's nucleotide sequence