Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1688

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1686 and Pf6N2E2_1687 overlap by 7 nucleotidesPf6N2E2_1687 and Pf6N2E2_1688 are separated by 35 nucleotidesPf6N2E2_1688 and Pf6N2E2_1689 are separated by 265 nucleotidesPf6N2E2_1689 and Pf6N2E2_1690 are separated by 33 nucleotides Pf6N2E2_1686 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,995,479 to 1,996,540 _1686 Pf6N2E2_1687 - putative iron ABC transporter ATP-binding protein, at 1,996,534 to 1,997,289 _1687 Pf6N2E2_1688 - Permeases of the major facilitator superfamily, at 1,997,325 to 1,998,581 _1688 Pf6N2E2_1689 - Probable porin, at 1,998,847 to 1,999,323 _1689 Pf6N2E2_1690 - Mobile element protein, at 1,999,357 to 2,000,526 _1690 Position (kb) 1997 1998 1999Strain fitness (log2 ratio) -1 0 1 2 3at 1996.753 kb on + strand, within Pf6N2E2_1687at 1996.753 kb on + strand, within Pf6N2E2_1687at 1996.754 kb on - strand, within Pf6N2E2_1687at 1996.754 kb on - strand, within Pf6N2E2_1687at 1996.754 kb on - strand, within Pf6N2E2_1687at 1996.754 kb on - strand, within Pf6N2E2_1687at 1996.795 kb on + strand, within Pf6N2E2_1687at 1996.796 kb on - strand, within Pf6N2E2_1687at 1996.796 kb on - strand, within Pf6N2E2_1687at 1996.796 kb on - strand, within Pf6N2E2_1687at 1996.796 kb on - strand, within Pf6N2E2_1687at 1997.087 kb on + strand, within Pf6N2E2_1687at 1997.087 kb on + strand, within Pf6N2E2_1687at 1997.088 kb on - strand, within Pf6N2E2_1687at 1997.189 kb on + strand, within Pf6N2E2_1687at 1997.189 kb on + strand, within Pf6N2E2_1687at 1997.190 kb on - strand, within Pf6N2E2_1687at 1997.218 kb on + strandat 1997.218 kb on + strandat 1997.218 kb on + strandat 1997.280 kb on + strandat 1997.326 kb on + strandat 1997.327 kb on - strandat 1997.327 kb on - strandat 1997.499 kb on - strand, within Pf6N2E2_1688at 1997.499 kb on - strand, within Pf6N2E2_1688at 1997.678 kb on - strand, within Pf6N2E2_1688at 1997.678 kb on - strand, within Pf6N2E2_1688at 1997.678 kb on - strand, within Pf6N2E2_1688at 1997.678 kb on - strand, within Pf6N2E2_1688at 1997.691 kb on + strand, within Pf6N2E2_1688at 1997.928 kb on - strand, within Pf6N2E2_1688at 1997.928 kb on - strand, within Pf6N2E2_1688at 1998.080 kb on - strand, within Pf6N2E2_1688at 1998.186 kb on - strand, within Pf6N2E2_1688at 1998.494 kb on - strandat 1998.494 kb on - strandat 1998.557 kb on + strandat 1998.557 kb on + strandat 1998.558 kb on - strandat 1998.831 kb on - strandat 1998.831 kb on - strandat 1998.831 kb on - strandat 1998.848 kb on + strandat 1998.848 kb on + strandat 1998.849 kb on - strandat 1998.849 kb on - strandat 1998.880 kb on - strandat 1998.880 kb on - strandat 1998.923 kb on + strand, within Pf6N2E2_1689at 1998.923 kb on + strand, within Pf6N2E2_1689at 1998.923 kb on + strand, within Pf6N2E2_1689at 1998.924 kb on - strand, within Pf6N2E2_1689at 1998.924 kb on - strand, within Pf6N2E2_1689at 1998.924 kb on - strand, within Pf6N2E2_1689at 1998.924 kb on - strand, within Pf6N2E2_1689at 1998.924 kb on - strand, within Pf6N2E2_1689at 1998.958 kb on - strand, within Pf6N2E2_1689at 1998.958 kb on - strand, within Pf6N2E2_1689at 1998.958 kb on - strand, within Pf6N2E2_1689at 1998.974 kb on + strand, within Pf6N2E2_1689at 1998.975 kb on - strand, within Pf6N2E2_1689at 1999.091 kb on + strand, within Pf6N2E2_1689at 1999.163 kb on + strand, within Pf6N2E2_1689at 1999.184 kb on + strand, within Pf6N2E2_1689at 1999.184 kb on + strand, within Pf6N2E2_1689at 1999.184 kb on + strand, within Pf6N2E2_1689at 1999.184 kb on + strand, within Pf6N2E2_1689at 1999.184 kb on + strand, within Pf6N2E2_1689at 1999.185 kb on - strand, within Pf6N2E2_1689at 1999.185 kb on - strand, within Pf6N2E2_1689at 1999.185 kb on - strand, within Pf6N2E2_1689at 1999.338 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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1,996,753 + Pf6N2E2_1687 0.29 +0.1
1,996,753 + Pf6N2E2_1687 0.29 +0.7
1,996,754 - Pf6N2E2_1687 0.29 -0.1
1,996,754 - Pf6N2E2_1687 0.29 +0.4
1,996,754 - Pf6N2E2_1687 0.29 +0.5
1,996,754 - Pf6N2E2_1687 0.29 -0.6
1,996,795 + Pf6N2E2_1687 0.35 +0.8
1,996,796 - Pf6N2E2_1687 0.35 +1.0
1,996,796 - Pf6N2E2_1687 0.35 -0.0
1,996,796 - Pf6N2E2_1687 0.35 -0.2
1,996,796 - Pf6N2E2_1687 0.35 +0.6
1,997,087 + Pf6N2E2_1687 0.73 +0.5
1,997,087 + Pf6N2E2_1687 0.73 +1.0
1,997,088 - Pf6N2E2_1687 0.73 -0.4
1,997,189 + Pf6N2E2_1687 0.87 +2.4
1,997,189 + Pf6N2E2_1687 0.87 -0.0
1,997,190 - Pf6N2E2_1687 0.87 -1.3
1,997,218 + +1.5
1,997,218 + -0.6
1,997,218 + +0.4
1,997,280 + +0.1
1,997,326 + +0.6
1,997,327 - +0.2
1,997,327 - +0.3
1,997,499 - Pf6N2E2_1688 0.14 +1.7
1,997,499 - Pf6N2E2_1688 0.14 -0.2
1,997,678 - Pf6N2E2_1688 0.28 +0.2
1,997,678 - Pf6N2E2_1688 0.28 +0.4
1,997,678 - Pf6N2E2_1688 0.28 +0.6
1,997,678 - Pf6N2E2_1688 0.28 +0.5
1,997,691 + Pf6N2E2_1688 0.29 +1.3
1,997,928 - Pf6N2E2_1688 0.48 +1.1
1,997,928 - Pf6N2E2_1688 0.48 -0.5
1,998,080 - Pf6N2E2_1688 0.60 +2.0
1,998,186 - Pf6N2E2_1688 0.68 +0.4
1,998,494 - -0.9
1,998,494 - -0.8
1,998,557 + -0.0
1,998,557 + +0.4
1,998,558 - -0.0
1,998,831 - +0.3
1,998,831 - +0.1
1,998,831 - -0.6
1,998,848 + +1.3
1,998,848 + -0.7
1,998,849 - +0.6
1,998,849 - +0.0
1,998,880 - -0.1
1,998,880 - -1.3
1,998,923 + Pf6N2E2_1689 0.16 -0.0
1,998,923 + Pf6N2E2_1689 0.16 +1.3
1,998,923 + Pf6N2E2_1689 0.16 +1.4
1,998,924 - Pf6N2E2_1689 0.16 -0.1
1,998,924 - Pf6N2E2_1689 0.16 +0.9
1,998,924 - Pf6N2E2_1689 0.16 +1.7
1,998,924 - Pf6N2E2_1689 0.16 +1.1
1,998,924 - Pf6N2E2_1689 0.16 +0.6
1,998,958 - Pf6N2E2_1689 0.23 -0.0
1,998,958 - Pf6N2E2_1689 0.23 +0.6
1,998,958 - Pf6N2E2_1689 0.23 +2.1
1,998,974 + Pf6N2E2_1689 0.27 +0.5
1,998,975 - Pf6N2E2_1689 0.27 -0.5
1,999,091 + Pf6N2E2_1689 0.51 +0.0
1,999,163 + Pf6N2E2_1689 0.66 +0.5
1,999,184 + Pf6N2E2_1689 0.71 -0.1
1,999,184 + Pf6N2E2_1689 0.71 -1.4
1,999,184 + Pf6N2E2_1689 0.71 -0.0
1,999,184 + Pf6N2E2_1689 0.71 -0.6
1,999,184 + Pf6N2E2_1689 0.71 +2.9
1,999,185 - Pf6N2E2_1689 0.71 -0.7
1,999,185 - Pf6N2E2_1689 0.71 -1.0
1,999,185 - Pf6N2E2_1689 0.71 -0.7
1,999,338 - -1.5

Or see this region's nucleotide sequence