Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1340 and Pf6N2E2_1341 are separated by 10 nucleotides Pf6N2E2_1341 and Pf6N2E2_1342 are separated by 23 nucleotides Pf6N2E2_1342 and Pf6N2E2_1343 are separated by 429 nucleotides
Pf6N2E2_1340 - major pilin protein fimA, at 1,576,360 to 1,577,034
_1340
Pf6N2E2_1341 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 1,577,045 to 1,577,431
_1341
Pf6N2E2_1342 - monooxygenase, putative, at 1,577,455 to 1,578,780
_1342
Pf6N2E2_1343 - Transcriptional regulator, at 1,579,210 to 1,580,124
_1343
Position (kb)
1577
1578
1579 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1576.899 kb on + strand, within Pf6N2E2_1340 at 1576.900 kb on - strand, within Pf6N2E2_1340 at 1576.990 kb on + strand at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.349 kb on + strand at 1577.349 kb on + strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341 at 1577.438 kb on - strand at 1577.546 kb on + strand at 1577.546 kb on + strand at 1577.546 kb on + strand at 1577.546 kb on + strand at 1577.547 kb on - strand at 1577.578 kb on - strand at 1577.578 kb on - strand at 1577.733 kb on - strand, within Pf6N2E2_1342 at 1577.885 kb on + strand, within Pf6N2E2_1342 at 1577.886 kb on - strand, within Pf6N2E2_1342 at 1577.916 kb on - strand, within Pf6N2E2_1342 at 1577.916 kb on - strand, within Pf6N2E2_1342 at 1578.042 kb on + strand, within Pf6N2E2_1342 at 1578.197 kb on + strand, within Pf6N2E2_1342 at 1578.197 kb on + strand, within Pf6N2E2_1342 at 1578.198 kb on - strand, within Pf6N2E2_1342 at 1578.512 kb on + strand, within Pf6N2E2_1342 at 1578.513 kb on - strand, within Pf6N2E2_1342 at 1578.800 kb on + strand at 1578.800 kb on + strand at 1578.800 kb on + strand at 1579.015 kb on + strand at 1579.072 kb on + strand at 1579.072 kb on + strand at 1579.111 kb on - strand at 1579.153 kb on + strand at 1579.153 kb on + strand at 1579.154 kb on - strand at 1579.341 kb on - strand, within Pf6N2E2_1343 at 1579.610 kb on + strand, within Pf6N2E2_1343 at 1579.610 kb on + strand, within Pf6N2E2_1343 at 1579.611 kb on - strand, within Pf6N2E2_1343 at 1579.668 kb on - strand, within Pf6N2E2_1343
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,576,899 + Pf6N2E2_1340 0.80 +0.9 1,576,900 - Pf6N2E2_1340 0.80 +0.9 1,576,990 + +0.3 1,577,199 + Pf6N2E2_1341 0.40 -1.4 1,577,199 + Pf6N2E2_1341 0.40 +0.8 1,577,199 + Pf6N2E2_1341 0.40 +0.4 1,577,199 + Pf6N2E2_1341 0.40 -2.0 1,577,200 - Pf6N2E2_1341 0.40 +0.3 1,577,200 - Pf6N2E2_1341 0.40 -0.5 1,577,200 - Pf6N2E2_1341 0.40 +0.3 1,577,349 + +1.2 1,577,349 + Pf6N2E2_1341 0.79 +2.4 1,577,350 - Pf6N2E2_1341 0.79 -3.1 1,577,350 - Pf6N2E2_1341 0.79 -1.6 1,577,438 - -3.1 1,577,546 + +1.2 1,577,546 + -1.1 1,577,546 + +0.0 1,577,546 + -0.7 1,577,547 - +1.2 1,577,578 - +0.5 1,577,578 - -0.1 1,577,733 - Pf6N2E2_1342 0.21 -2.1 1,577,885 + Pf6N2E2_1342 0.32 -1.6 1,577,886 - Pf6N2E2_1342 0.33 -0.1 1,577,916 - Pf6N2E2_1342 0.35 +0.8 1,577,916 - Pf6N2E2_1342 0.35 +1.1 1,578,042 + Pf6N2E2_1342 0.44 -0.2 1,578,197 + Pf6N2E2_1342 0.56 +3.8 1,578,197 + Pf6N2E2_1342 0.56 +2.3 1,578,198 - Pf6N2E2_1342 0.56 +0.8 1,578,512 + Pf6N2E2_1342 0.80 +1.2 1,578,513 - Pf6N2E2_1342 0.80 +1.1 1,578,800 + -0.3 1,578,800 + +0.1 1,578,800 + +0.6 1,579,015 + +0.2 1,579,072 + +0.5 1,579,072 + -1.1 1,579,111 - -1.1 1,579,153 + -0.1 1,579,153 + +0.4 1,579,154 - +0.0 1,579,341 - Pf6N2E2_1343 0.14 -0.6 1,579,610 + Pf6N2E2_1343 0.44 -1.1 1,579,610 + Pf6N2E2_1343 0.44 -0.2 1,579,611 - Pf6N2E2_1343 0.44 +0.1 1,579,668 - Pf6N2E2_1343 0.50 +0.3
Or see this region's nucleotide sequence