Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_115 and Pf6N2E2_116 are separated by 12 nucleotides Pf6N2E2_116 and Pf6N2E2_117 overlap by 8 nucleotides Pf6N2E2_117 and Pf6N2E2_118 overlap by 4 nucleotides
Pf6N2E2_115 - FIG00958408: hypothetical protein, at 161,631 to 162,635
_115
Pf6N2E2_116 - Glycosyltransferase, at 162,648 to 163,784
_116
Pf6N2E2_117 - Glycosyl transferase, group 2 family protein, at 163,777 to 164,736
_117
Pf6N2E2_118 - FIG00961753: hypothetical protein, at 164,733 to 166,103
_118
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 161.741 kb on - strand, within Pf6N2E2_115 at 161.828 kb on - strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 162.085 kb on + strand, within Pf6N2E2_115 at 162.086 kb on - strand, within Pf6N2E2_115 at 162.086 kb on - strand at 162.278 kb on + strand, within Pf6N2E2_115 at 162.812 kb on - strand, within Pf6N2E2_116 at 162.844 kb on + strand, within Pf6N2E2_116 at 162.949 kb on + strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.579 kb on + strand, within Pf6N2E2_116 at 163.735 kb on + strand at 163.741 kb on + strand at 163.742 kb on - strand at 163.768 kb on + strand at 163.769 kb on - strand at 164.705 kb on + strand at 164.705 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 161,741 - Pf6N2E2_115 0.11 -1.2 161,828 - Pf6N2E2_115 0.20 +0.6 161,833 + Pf6N2E2_115 0.20 -1.9 161,833 + Pf6N2E2_115 0.20 +0.4 161,833 + Pf6N2E2_115 0.20 +0.3 161,833 + Pf6N2E2_115 0.20 +1.9 161,834 - Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 -1.2 161,834 - Pf6N2E2_115 0.20 -1.0 161,834 - Pf6N2E2_115 0.20 -0.0 161,834 - Pf6N2E2_115 0.20 -0.6 161,834 - Pf6N2E2_115 0.20 -0.8 161,834 - Pf6N2E2_115 0.20 -0.4 161,834 - Pf6N2E2_115 0.20 -0.1 161,834 - Pf6N2E2_115 0.20 -0.6 162,085 + Pf6N2E2_115 0.45 +0.2 162,086 - Pf6N2E2_115 0.45 -0.1 162,086 - +2.1 162,278 + Pf6N2E2_115 0.64 -0.8 162,812 - Pf6N2E2_116 0.14 +1.0 162,844 + Pf6N2E2_116 0.17 +0.8 162,949 + Pf6N2E2_116 0.26 +1.9 163,091 - Pf6N2E2_116 0.39 +0.8 163,091 - Pf6N2E2_116 0.39 +0.9 163,256 - Pf6N2E2_116 0.53 -1.6 163,256 - Pf6N2E2_116 0.53 +0.4 163,579 + Pf6N2E2_116 0.82 -0.1 163,735 + +0.9 163,741 + -0.2 163,742 - -1.4 163,768 + -0.7 163,769 - -0.5 164,705 + -0.0 164,705 + -2.0
Or see this region's nucleotide sequence