Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0672 and Shew_0673 are separated by 8 nucleotides Shew_0673 and Shew_0674 are separated by 98 nucleotides Shew_0674 and Shew_0675 are separated by 202 nucleotides
Shew_0672: Shew_0672 - uroporphyrin-III C-methyltransferase (RefSeq), at 765,289 to 766,110
_0672
Shew_0673: Shew_0673 - hypothetical protein (RefSeq), at 766,119 to 766,955
_0673
Shew_0674: Shew_0674 - major facilitator transporter (RefSeq), at 767,054 to 768,220
_0674
Shew_0675: Shew_0675 - hypothetical protein (RefSeq), at 768,423 to 769,868
_0675
Position (kb) 767
768
769 Strain fitness (log2 ratio) -1
0
1 at 766.160 kb on - strand at 766.292 kb on + strand, within Shew_0673 at 766.365 kb on + strand, within Shew_0673 at 766.526 kb on - strand, within Shew_0673 at 766.618 kb on + strand, within Shew_0673 at 766.724 kb on - strand, within Shew_0673 at 766.935 kb on - strand at 767.040 kb on - strand at 767.046 kb on + strand at 767.143 kb on - strand at 767.187 kb on + strand, within Shew_0674 at 767.360 kb on - strand, within Shew_0674 at 767.645 kb on + strand, within Shew_0674 at 767.780 kb on + strand, within Shew_0674 at 767.886 kb on - strand, within Shew_0674 at 767.912 kb on + strand, within Shew_0674 at 768.450 kb on - strand at 768.701 kb on - strand, within Shew_0675 at 768.783 kb on + strand, within Shew_0675 at 769.087 kb on - strand, within Shew_0675
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 766,160 - +1.3 766,292 + Shew_0673 0.21 +0.2 766,365 + Shew_0673 0.29 -0.5 766,526 - Shew_0673 0.49 +0.3 766,618 + Shew_0673 0.60 -0.5 766,724 - Shew_0673 0.72 +0.3 766,935 - -0.0 767,040 - -0.1 767,046 + -0.0 767,143 - +0.4 767,187 + Shew_0674 0.11 -0.1 767,360 - Shew_0674 0.26 -0.0 767,645 + Shew_0674 0.51 +0.3 767,780 + Shew_0674 0.62 -1.3 767,886 - Shew_0674 0.71 -0.4 767,912 + Shew_0674 0.74 +0.0 768,450 - +0.2 768,701 - Shew_0675 0.19 -0.2 768,783 + Shew_0675 0.25 -0.2 769,087 - Shew_0675 0.46 +0.3
Or see this region's nucleotide sequence