Experiment: Nickel (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2512 and Shew_2513 are separated by 18 nucleotides Shew_2513 and Shew_2514 are separated by 73 nucleotides Shew_2514 and Shew_2515 are separated by 250 nucleotides Shew_2515 and Shew_2516 are separated by 83 nucleotides
Shew_2512: Shew_2512 - peptidylprolyl isomerase (RefSeq), at 2,956,409 to 2,956,921
_2512
Shew_2513: Shew_2513 - UDP-2,3-diacylglucosamine hydrolase (RefSeq), at 2,956,940 to 2,957,668
_2513
Shew_2514: Shew_2514 - tRNA--hydroxylase (RefSeq), at 2,957,742 to 2,958,512
_2514
Shew_2515: Shew_2515 - hypothetical protein (RefSeq), at 2,958,763 to 2,959,359
_2515
Shew_2516: Shew_2516 - glutaminyl-tRNA synthetase (RefSeq), at 2,959,443 to 2,961,113
_2516
Position (kb)
2957
2958
2959 Strain fitness (log2 ratio)
-1
0
1 at 2958.029 kb on + strand, within Shew_2514 at 2958.102 kb on - strand, within Shew_2514 at 2958.196 kb on + strand, within Shew_2514 at 2959.193 kb on + strand, within Shew_2515 at 2959.216 kb on - strand, within Shew_2515 at 2959.216 kb on - strand, within Shew_2515 at 2959.216 kb on - strand, within Shew_2515 at 2959.277 kb on + strand, within Shew_2515 at 2959.282 kb on + strand, within Shew_2515 at 2959.411 kb on - strand at 2959.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.5 mM remove 2,958,029 + Shew_2514 0.37 -1.6 2,958,102 - Shew_2514 0.47 -0.6 2,958,196 + Shew_2514 0.59 +0.1 2,959,193 + Shew_2515 0.72 -0.2 2,959,216 - Shew_2515 0.76 +0.6 2,959,216 - Shew_2515 0.76 +0.0 2,959,216 - Shew_2515 0.76 -0.0 2,959,277 + Shew_2515 0.86 -0.3 2,959,282 + Shew_2515 0.87 +0.0 2,959,411 - -0.2 2,959,433 - +1.0
Or see this region's nucleotide sequence