Experiment: m.b. Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4118 and Dshi_4119 are separated by 131 nucleotides Dshi_4119 and Dshi_4120 overlap by 4 nucleotides Dshi_4120 and Dshi_4121 are separated by 147 nucleotides
Dshi_4118: Dshi_4118 - glucose-1-phosphate thymidylyltransferase (RefSeq), at 49,744 to 50,616
_4118
Dshi_4119: Dshi_4119 - NAD-dependent epimerase/dehydratase (RefSeq), at 50,748 to 51,629
_4119
Dshi_4120: Dshi_4120 - sugar transferase (RefSeq), at 51,626 to 52,234
_4120
Dshi_4121: Dshi_4121 - glycosyl transferase family 2 (RefSeq), at 52,382 to 54,934
_4121
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 49.791 kb on + strand at 49.799 kb on - strand at 49.834 kb on + strand, within Dshi_4118 at 50.053 kb on + strand, within Dshi_4118 at 50.073 kb on - strand, within Dshi_4118 at 50.098 kb on - strand, within Dshi_4118 at 50.154 kb on - strand, within Dshi_4118 at 50.191 kb on + strand, within Dshi_4118 at 50.311 kb on + strand, within Dshi_4118 at 50.323 kb on + strand, within Dshi_4118 at 50.350 kb on + strand, within Dshi_4118 at 50.501 kb on + strand, within Dshi_4118 at 50.563 kb on - strand at 50.594 kb on + strand at 50.672 kb on + strand at 50.680 kb on - strand at 50.701 kb on - strand at 50.766 kb on + strand at 51.188 kb on + strand, within Dshi_4119 at 51.189 kb on + strand, within Dshi_4119 at 51.375 kb on + strand, within Dshi_4119 at 51.375 kb on + strand, within Dshi_4119 at 52.118 kb on + strand, within Dshi_4120 at 52.146 kb on + strand, within Dshi_4120 at 52.273 kb on + strand at 52.306 kb on - strand at 52.390 kb on + strand at 52.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 0.005 mg/ml remove 49,791 + +0.4 49,799 - +0.5 49,834 + Dshi_4118 0.10 -0.9 50,053 + Dshi_4118 0.35 -0.1 50,073 - Dshi_4118 0.38 -0.1 50,098 - Dshi_4118 0.41 -0.0 50,154 - Dshi_4118 0.47 -0.4 50,191 + Dshi_4118 0.51 -0.1 50,311 + Dshi_4118 0.65 -0.8 50,323 + Dshi_4118 0.66 +0.5 50,350 + Dshi_4118 0.69 -0.6 50,501 + Dshi_4118 0.87 -0.6 50,563 - +0.3 50,594 + +0.4 50,672 + -0.3 50,680 - -0.5 50,701 - +0.3 50,766 + -0.8 51,188 + Dshi_4119 0.50 -1.8 51,189 + Dshi_4119 0.50 -3.1 51,375 + Dshi_4119 0.71 -1.9 51,375 + Dshi_4119 0.71 -1.1 52,118 + Dshi_4120 0.81 -0.3 52,146 + Dshi_4120 0.85 -2.0 52,273 + +0.3 52,306 - +0.5 52,390 + -0.1 52,489 - -0.1
Or see this region's nucleotide sequence