Experiment: m.b. Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1282 and Dshi_1283 are separated by 129 nucleotides Dshi_1283 and Dshi_1284 are separated by 58 nucleotides Dshi_1284 and Dshi_1285 overlap by 4 nucleotides
Dshi_1282: Dshi_1282 - formate dehydrogenase, alpha subunit (RefSeq), at 1,332,581 to 1,335,382
_1282
Dshi_1283: Dshi_1283 - Formate--tetrahydrofolate ligase (RefSeq), at 1,335,512 to 1,337,179
_1283
Dshi_1284: Dshi_1284 - chorismate mutase related enzyme (RefSeq), at 1,337,238 to 1,337,558
_1284
Dshi_1285: Dshi_1285 - Methylenetetrahydrofolate dehydrogenase (NADP(+)) (RefSeq), at 1,337,555 to 1,338,454
_1285
Position (kb)
1335
1336
1337
1338 Strain fitness (log2 ratio)
-1
0
1 at 1334.557 kb on - strand, within Dshi_1282 at 1334.771 kb on + strand, within Dshi_1282 at 1335.266 kb on + strand at 1335.616 kb on - strand at 1335.750 kb on + strand, within Dshi_1283 at 1335.843 kb on + strand, within Dshi_1283 at 1336.149 kb on + strand, within Dshi_1283 at 1336.167 kb on - strand, within Dshi_1283 at 1336.487 kb on + strand, within Dshi_1283 at 1336.877 kb on - strand, within Dshi_1283 at 1336.941 kb on + strand, within Dshi_1283 at 1336.942 kb on - strand, within Dshi_1283 at 1336.966 kb on + strand, within Dshi_1283 at 1337.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 0.005 mg/ml remove 1,334,557 - Dshi_1282 0.71 -1.2 1,334,771 + Dshi_1282 0.78 -0.4 1,335,266 + +0.7 1,335,616 - -0.4 1,335,750 + Dshi_1283 0.14 -1.6 1,335,843 + Dshi_1283 0.20 -0.3 1,336,149 + Dshi_1283 0.38 -0.7 1,336,167 - Dshi_1283 0.39 -0.6 1,336,487 + Dshi_1283 0.58 +0.3 1,336,877 - Dshi_1283 0.82 -1.5 1,336,941 + Dshi_1283 0.86 -0.9 1,336,942 - Dshi_1283 0.86 -1.5 1,336,966 + Dshi_1283 0.87 +1.1 1,337,088 - -1.2
Or see this region's nucleotide sequence