Experiment: m.b. Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0979 and Dshi_0980 overlap by 4 nucleotides Dshi_0980 and Dshi_0981 are separated by 3 nucleotides Dshi_0981 and Dshi_0982 are separated by 149 nucleotides Dshi_0982 and Dshi_0983 are separated by 2 nucleotides
Dshi_0979: Dshi_0979 - Histidinol dehydrogenase (RefSeq), at 1,000,537 to 1,001,853
_0979
Dshi_0980: Dshi_0980 - hypothetical protein (RefSeq), at 1,001,850 to 1,002,329
_0980
Dshi_0981: Dshi_0981 - protein tyrosine phosphatase (RefSeq), at 1,002,333 to 1,002,788
_0981
Dshi_0982: Dshi_0982 - translation initiation factor IF-1 (RefSeq), at 1,002,938 to 1,003,156
_0982
Dshi_0983: Dshi_0983 - maf protein (RefSeq), at 1,003,159 to 1,003,743
_0983
Position (kb)
1001
1002
1003 Strain fitness (log2 ratio)
-1
0
1 at 1001.934 kb on + strand, within Dshi_0980 at 1002.004 kb on + strand, within Dshi_0980 at 1002.010 kb on + strand, within Dshi_0980 at 1002.060 kb on + strand, within Dshi_0980 at 1002.167 kb on + strand, within Dshi_0980 at 1002.175 kb on - strand, within Dshi_0980 at 1002.190 kb on - strand, within Dshi_0980 at 1002.201 kb on + strand, within Dshi_0980 at 1002.459 kb on + strand, within Dshi_0981 at 1002.567 kb on + strand, within Dshi_0981 at 1002.611 kb on + strand, within Dshi_0981 at 1002.627 kb on - strand, within Dshi_0981 at 1002.659 kb on - strand, within Dshi_0981 at 1002.659 kb on - strand, within Dshi_0981 at 1002.687 kb on + strand, within Dshi_0981 at 1002.720 kb on + strand, within Dshi_0981 at 1002.723 kb on - strand, within Dshi_0981 at 1003.232 kb on - strand, within Dshi_0983
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 0.005 mg/ml remove 1,001,934 + Dshi_0980 0.17 -1.2 1,002,004 + Dshi_0980 0.32 -0.7 1,002,010 + Dshi_0980 0.33 -1.1 1,002,060 + Dshi_0980 0.44 -0.1 1,002,167 + Dshi_0980 0.66 -1.0 1,002,175 - Dshi_0980 0.68 -1.4 1,002,190 - Dshi_0980 0.71 -0.3 1,002,201 + Dshi_0980 0.73 -0.9 1,002,459 + Dshi_0981 0.28 +0.2 1,002,567 + Dshi_0981 0.51 +0.2 1,002,611 + Dshi_0981 0.61 +0.6 1,002,627 - Dshi_0981 0.64 +0.4 1,002,659 - Dshi_0981 0.71 +0.6 1,002,659 - Dshi_0981 0.71 +1.4 1,002,687 + Dshi_0981 0.78 +0.2 1,002,720 + Dshi_0981 0.85 +0.4 1,002,723 - Dshi_0981 0.86 -0.2 1,003,232 - Dshi_0983 0.12 +0.1
Or see this region's nucleotide sequence