Experiment: m.b. Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0015 and Dshi_0016 are separated by 451 nucleotides Dshi_0016 and Dshi_0017 are separated by 89 nucleotides Dshi_0017 and Dshi_0018 are separated by 159 nucleotides
Dshi_0015: Dshi_0015 - ribonuclease R (RefSeq), at 15,472 to 17,727
_0015
Dshi_0016: Dshi_0016 - lytic murein transglycosylase (RefSeq), at 18,179 to 19,426
_0016
Dshi_0017: Dshi_0017 - hypothetical protein (RefSeq), at 19,516 to 20,166
_0017
Dshi_0018: Dshi_0018 - multicopper oxidase type 2 (RefSeq), at 20,326 to 22,629
_0018
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1 at 17.196 kb on - strand, within Dshi_0015 at 17.289 kb on + strand, within Dshi_0015 at 17.319 kb on + strand, within Dshi_0015 at 17.373 kb on + strand, within Dshi_0015 at 17.390 kb on + strand, within Dshi_0015 at 17.391 kb on + strand, within Dshi_0015 at 17.418 kb on + strand, within Dshi_0015 at 17.585 kb on + strand at 17.721 kb on + strand at 17.729 kb on - strand at 17.742 kb on - strand at 17.742 kb on - strand at 17.755 kb on - strand at 17.865 kb on - strand at 17.877 kb on - strand at 17.970 kb on - strand at 18.096 kb on - strand at 18.176 kb on - strand at 18.184 kb on - strand at 18.246 kb on + strand at 18.287 kb on + strand at 18.297 kb on - strand at 18.309 kb on - strand, within Dshi_0016 at 18.468 kb on - strand, within Dshi_0016 at 18.478 kb on - strand, within Dshi_0016 at 18.532 kb on + strand, within Dshi_0016 at 18.611 kb on + strand, within Dshi_0016 at 18.768 kb on + strand, within Dshi_0016 at 18.769 kb on + strand, within Dshi_0016 at 18.770 kb on - strand, within Dshi_0016 at 18.791 kb on - strand, within Dshi_0016 at 18.883 kb on + strand, within Dshi_0016 at 18.931 kb on + strand, within Dshi_0016 at 18.939 kb on - strand, within Dshi_0016 at 18.939 kb on - strand, within Dshi_0016 at 18.939 kb on - strand, within Dshi_0016 at 18.975 kb on + strand, within Dshi_0016 at 19.150 kb on + strand, within Dshi_0016 at 19.152 kb on - strand at 19.181 kb on + strand, within Dshi_0016 at 19.218 kb on - strand, within Dshi_0016 at 19.223 kb on + strand, within Dshi_0016 at 19.257 kb on - strand, within Dshi_0016 at 19.361 kb on - strand at 19.368 kb on + strand at 19.376 kb on - strand at 19.389 kb on + strand at 19.477 kb on + strand at 19.498 kb on + strand at 19.506 kb on - strand at 19.599 kb on - strand, within Dshi_0017 at 19.680 kb on - strand, within Dshi_0017 at 19.924 kb on + strand, within Dshi_0017 at 20.047 kb on - strand, within Dshi_0017 at 20.071 kb on - strand, within Dshi_0017 at 20.112 kb on + strand at 20.120 kb on - strand at 20.172 kb on - strand at 20.270 kb on - strand at 20.373 kb on + strand at 20.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 0.005 mg/ml remove 17,196 - Dshi_0015 0.76 -0.2 17,289 + Dshi_0015 0.81 -0.4 17,319 + Dshi_0015 0.82 +0.1 17,373 + Dshi_0015 0.84 +0.3 17,390 + Dshi_0015 0.85 -0.8 17,391 + Dshi_0015 0.85 +0.4 17,418 + Dshi_0015 0.86 -0.2 17,585 + -1.3 17,721 + -1.6 17,729 - +0.1 17,742 - -0.8 17,742 - -0.7 17,755 - +0.6 17,865 - -0.3 17,877 - -0.3 17,970 - -0.4 18,096 - -0.6 18,176 - -0.6 18,184 - -0.3 18,246 + -1.2 18,287 + -0.8 18,297 - +0.2 18,309 - Dshi_0016 0.10 +0.2 18,468 - Dshi_0016 0.23 +0.1 18,478 - Dshi_0016 0.24 +0.7 18,532 + Dshi_0016 0.28 +0.2 18,611 + Dshi_0016 0.35 +1.2 18,768 + Dshi_0016 0.47 -0.3 18,769 + Dshi_0016 0.47 -0.2 18,770 - Dshi_0016 0.47 -0.1 18,791 - Dshi_0016 0.49 -0.5 18,883 + Dshi_0016 0.56 -0.6 18,931 + Dshi_0016 0.60 -0.1 18,939 - Dshi_0016 0.61 +0.6 18,939 - Dshi_0016 0.61 -0.8 18,939 - Dshi_0016 0.61 -2.2 18,975 + Dshi_0016 0.64 +0.1 19,150 + Dshi_0016 0.78 +0.5 19,152 - -0.4 19,181 + Dshi_0016 0.80 -1.0 19,218 - Dshi_0016 0.83 -2.6 19,223 + Dshi_0016 0.84 -0.0 19,257 - Dshi_0016 0.86 -0.2 19,361 - +1.6 19,368 + +0.8 19,376 - +0.2 19,389 + -0.6 19,477 + +0.0 19,498 + -0.8 19,506 - +0.8 19,599 - Dshi_0017 0.13 -0.7 19,680 - Dshi_0017 0.25 -0.2 19,924 + Dshi_0017 0.63 -1.1 20,047 - Dshi_0017 0.82 -0.3 20,071 - Dshi_0017 0.85 -0.6 20,112 + -0.2 20,120 - -0.5 20,172 - +0.2 20,270 - -1.0 20,373 + -1.0 20,415 + -0.8
Or see this region's nucleotide sequence