Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5942 and Pf6N2E2_5943 overlap by 21 nucleotides Pf6N2E2_5943 and Pf6N2E2_5944 are separated by 312 nucleotides Pf6N2E2_5944 and Pf6N2E2_5945 overlap by 11 nucleotides Pf6N2E2_5945 and Pf6N2E2_5946 are separated by 20 nucleotides
Pf6N2E2_5942 - Ribonuclease E (EC 3.1.26.12), at 6,761,732 to 6,764,929
_5942
Pf6N2E2_5943 - hypothetical protein, at 6,764,909 to 6,765,313
_5943
Pf6N2E2_5944 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 6,765,626 to 6,766,594
_5944
Pf6N2E2_5945 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 6,766,584 to 6,767,246
_5945
Pf6N2E2_5946 - Periplasmic serine proteases (ClpP class), at 6,767,267 to 6,768,256
_5946
Position (kb)
6765
6766
6767 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.269 kb on - strand, within Pf6N2E2_5943 at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.307 kb on - strand at 6765.421 kb on - strand at 6765.598 kb on - strand at 6765.625 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.646 kb on + strand at 6765.647 kb on - strand at 6765.716 kb on - strand at 6765.818 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.928 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.932 kb on + strand, within Pf6N2E2_5944 at 6765.973 kb on + strand, within Pf6N2E2_5944 at 6766.170 kb on - strand, within Pf6N2E2_5944 at 6766.387 kb on + strand, within Pf6N2E2_5944 at 6766.387 kb on + strand, within Pf6N2E2_5944 at 6766.388 kb on - strand, within Pf6N2E2_5944 at 6766.428 kb on - strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.493 kb on + strand, within Pf6N2E2_5944 at 6766.494 kb on - strand, within Pf6N2E2_5944 at 6766.596 kb on + strand at 6766.689 kb on + strand, within Pf6N2E2_5945 at 6766.708 kb on + strand, within Pf6N2E2_5945 at 6766.748 kb on + strand, within Pf6N2E2_5945 at 6766.818 kb on + strand, within Pf6N2E2_5945 at 6766.819 kb on - strand, within Pf6N2E2_5945 at 6766.896 kb on + strand, within Pf6N2E2_5945 at 6766.971 kb on + strand, within Pf6N2E2_5945 at 6766.971 kb on + strand, within Pf6N2E2_5945 at 6766.972 kb on - strand, within Pf6N2E2_5945 at 6767.145 kb on + strand, within Pf6N2E2_5945 at 6767.393 kb on - strand, within Pf6N2E2_5946 at 6767.393 kb on - strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.416 kb on + strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.417 kb on - strand, within Pf6N2E2_5946 at 6767.420 kb on + strand, within Pf6N2E2_5946 at 6767.421 kb on - strand, within Pf6N2E2_5946 at 6767.421 kb on - strand, within Pf6N2E2_5946 at 6767.493 kb on - strand, within Pf6N2E2_5946 at 6767.493 kb on - strand, within Pf6N2E2_5946
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 6,765,269 - Pf6N2E2_5943 0.89 +0.0 6,765,269 - Pf6N2E2_5943 0.89 +0.3 6,765,269 - Pf6N2E2_5943 0.89 -0.2 6,765,307 - +0.8 6,765,307 - -1.4 6,765,307 - -0.1 6,765,307 - -1.1 6,765,421 - +0.6 6,765,598 - -0.6 6,765,625 + +0.2 6,765,646 + +0.8 6,765,646 + -1.1 6,765,646 + -0.9 6,765,646 + -1.7 6,765,647 - -1.7 6,765,716 - -0.8 6,765,818 + Pf6N2E2_5944 0.20 -0.2 6,765,928 + Pf6N2E2_5944 0.31 -0.1 6,765,928 + Pf6N2E2_5944 0.31 +0.1 6,765,928 + Pf6N2E2_5944 0.31 -1.8 6,765,932 + Pf6N2E2_5944 0.32 -2.5 6,765,932 + Pf6N2E2_5944 0.32 -1.4 6,765,932 + Pf6N2E2_5944 0.32 -0.2 6,765,973 + Pf6N2E2_5944 0.36 -0.6 6,766,170 - Pf6N2E2_5944 0.56 -0.2 6,766,387 + Pf6N2E2_5944 0.79 -0.1 6,766,387 + Pf6N2E2_5944 0.79 -0.4 6,766,388 - Pf6N2E2_5944 0.79 -1.4 6,766,428 - Pf6N2E2_5944 0.83 -1.1 6,766,493 + Pf6N2E2_5944 0.89 -1.2 6,766,493 + Pf6N2E2_5944 0.89 +0.8 6,766,493 + Pf6N2E2_5944 0.89 -1.2 6,766,493 + Pf6N2E2_5944 0.89 -1.7 6,766,493 + Pf6N2E2_5944 0.89 -0.6 6,766,493 + Pf6N2E2_5944 0.89 -1.7 6,766,493 + Pf6N2E2_5944 0.89 -2.3 6,766,493 + Pf6N2E2_5944 0.89 +0.2 6,766,493 + Pf6N2E2_5944 0.89 +0.6 6,766,493 + Pf6N2E2_5944 0.89 -1.6 6,766,493 + Pf6N2E2_5944 0.89 +0.1 6,766,494 - Pf6N2E2_5944 0.90 -0.0 6,766,596 + -0.3 6,766,689 + Pf6N2E2_5945 0.16 -1.8 6,766,708 + Pf6N2E2_5945 0.19 +0.2 6,766,748 + Pf6N2E2_5945 0.25 +1.8 6,766,818 + Pf6N2E2_5945 0.35 -0.2 6,766,819 - Pf6N2E2_5945 0.35 +0.0 6,766,896 + Pf6N2E2_5945 0.47 +0.6 6,766,971 + Pf6N2E2_5945 0.58 -1.3 6,766,971 + Pf6N2E2_5945 0.58 -0.8 6,766,972 - Pf6N2E2_5945 0.59 +0.3 6,767,145 + Pf6N2E2_5945 0.85 +0.1 6,767,393 - Pf6N2E2_5946 0.13 -2.2 6,767,393 - Pf6N2E2_5946 0.13 -1.8 6,767,416 + Pf6N2E2_5946 0.15 +0.3 6,767,416 + Pf6N2E2_5946 0.15 -1.8 6,767,416 + Pf6N2E2_5946 0.15 -0.8 6,767,417 - Pf6N2E2_5946 0.15 -0.8 6,767,417 - Pf6N2E2_5946 0.15 -0.1 6,767,417 - Pf6N2E2_5946 0.15 -0.2 6,767,417 - Pf6N2E2_5946 0.15 -1.1 6,767,417 - Pf6N2E2_5946 0.15 -0.0 6,767,417 - Pf6N2E2_5946 0.15 -0.8 6,767,417 - Pf6N2E2_5946 0.15 +0.8 6,767,417 - Pf6N2E2_5946 0.15 +0.3 6,767,417 - Pf6N2E2_5946 0.15 +0.2 6,767,417 - Pf6N2E2_5946 0.15 -0.1 6,767,420 + Pf6N2E2_5946 0.15 -1.7 6,767,421 - Pf6N2E2_5946 0.16 +0.1 6,767,421 - Pf6N2E2_5946 0.16 -0.9 6,767,493 - Pf6N2E2_5946 0.23 +0.8 6,767,493 - Pf6N2E2_5946 0.23 -0.8
Or see this region's nucleotide sequence