Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5814 and Pf6N2E2_5815 are separated by 8 nucleotides Pf6N2E2_5815 and Pf6N2E2_5816 are separated by 77 nucleotides Pf6N2E2_5816 and Pf6N2E2_5817 are separated by 73 nucleotides Pf6N2E2_5817 and Pf6N2E2_5818 are separated by 130 nucleotides
Pf6N2E2_5814 - Glutaredoxin, at 6,633,782 to 6,634,249
_5814
Pf6N2E2_5815 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 6,634,258 to 6,634,803
_5815
Pf6N2E2_5816 - Smr domain protein, at 6,634,881 to 6,635,438
_5816
Pf6N2E2_5817 - FIG00956876: hypothetical protein, at 6,635,512 to 6,635,832
_5817
Pf6N2E2_5818 - Isochorismatase (EC 3.3.2.1), at 6,635,963 to 6,636,553
_5818
Position (kb)
6634
6635
6636 Strain fitness (log2 ratio)
-2
-1
0
1 at 6633.956 kb on + strand, within Pf6N2E2_5814 at 6634.017 kb on + strand, within Pf6N2E2_5814 at 6634.157 kb on - strand, within Pf6N2E2_5814 at 6634.160 kb on - strand, within Pf6N2E2_5814 at 6634.160 kb on - strand, within Pf6N2E2_5814 at 6634.160 kb on - strand, within Pf6N2E2_5814 at 6634.195 kb on + strand, within Pf6N2E2_5814 at 6634.196 kb on - strand, within Pf6N2E2_5814 at 6634.259 kb on + strand at 6634.260 kb on - strand at 6634.882 kb on + strand at 6634.883 kb on - strand at 6634.883 kb on - strand at 6635.031 kb on - strand, within Pf6N2E2_5816 at 6635.031 kb on - strand, within Pf6N2E2_5816 at 6636.063 kb on + strand, within Pf6N2E2_5818
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 6,633,956 + Pf6N2E2_5814 0.37 -1.1 6,634,017 + Pf6N2E2_5814 0.50 -0.8 6,634,157 - Pf6N2E2_5814 0.80 -0.2 6,634,160 - Pf6N2E2_5814 0.81 -0.4 6,634,160 - Pf6N2E2_5814 0.81 +1.0 6,634,160 - Pf6N2E2_5814 0.81 +0.5 6,634,195 + Pf6N2E2_5814 0.88 +0.8 6,634,196 - Pf6N2E2_5814 0.88 -0.6 6,634,259 + -1.9 6,634,260 - -1.0 6,634,882 + -0.5 6,634,883 - +0.8 6,634,883 - +1.2 6,635,031 - Pf6N2E2_5816 0.27 -0.7 6,635,031 - Pf6N2E2_5816 0.27 -0.7 6,636,063 + Pf6N2E2_5818 0.17 -1.4
Or see this region's nucleotide sequence