Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4454 and Pf6N2E2_4455 are separated by 250 nucleotides Pf6N2E2_4455 and Pf6N2E2_4456 overlap by 14 nucleotides Pf6N2E2_4456 and Pf6N2E2_4457 overlap by 4 nucleotides
Pf6N2E2_4454 - Exopolyphosphatase (EC 3.6.1.11), at 5,186,421 to 5,187,923
_4454
Pf6N2E2_4455 - Glutamate transport ATP-binding protein, at 5,188,174 to 5,188,908
_4455
Pf6N2E2_4456 - Putative amino acid ABC transporter, permease protein, at 5,188,895 to 5,189,545
_4456
Pf6N2E2_4457 - amino acid ABC transporter, permease protein, at 5,189,542 to 5,190,222
_4457
Position (kb)
5188
5189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5187.350 kb on + strand, within Pf6N2E2_4454 at 5187.350 kb on + strand, within Pf6N2E2_4454 at 5187.350 kb on + strand, within Pf6N2E2_4454 at 5187.351 kb on - strand, within Pf6N2E2_4454 at 5187.351 kb on - strand, within Pf6N2E2_4454 at 5187.351 kb on - strand, within Pf6N2E2_4454 at 5187.352 kb on + strand, within Pf6N2E2_4454 at 5187.352 kb on + strand, within Pf6N2E2_4454 at 5187.352 kb on + strand, within Pf6N2E2_4454 at 5187.353 kb on - strand, within Pf6N2E2_4454 at 5187.353 kb on - strand, within Pf6N2E2_4454 at 5187.398 kb on - strand, within Pf6N2E2_4454 at 5187.409 kb on + strand, within Pf6N2E2_4454 at 5187.410 kb on - strand, within Pf6N2E2_4454 at 5187.692 kb on + strand, within Pf6N2E2_4454 at 5187.693 kb on - strand, within Pf6N2E2_4454 at 5188.109 kb on + strand at 5188.109 kb on + strand at 5188.180 kb on + strand at 5188.180 kb on + strand at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.592 kb on - strand, within Pf6N2E2_4455 at 5188.592 kb on - strand, within Pf6N2E2_4455 at 5188.712 kb on + strand, within Pf6N2E2_4455 at 5188.712 kb on + strand, within Pf6N2E2_4455 at 5188.713 kb on - strand, within Pf6N2E2_4455 at 5188.872 kb on - strand at 5188.872 kb on - strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.878 kb on + strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.896 kb on + strand at 5188.896 kb on + strand at 5188.896 kb on + strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.965 kb on + strand, within Pf6N2E2_4456 at 5188.965 kb on + strand, within Pf6N2E2_4456 at 5188.966 kb on - strand, within Pf6N2E2_4456 at 5188.986 kb on + strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5189.149 kb on - strand, within Pf6N2E2_4456 at 5189.248 kb on - strand, within Pf6N2E2_4456 at 5189.258 kb on - strand, within Pf6N2E2_4456 at 5189.258 kb on - strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.380 kb on - strand, within Pf6N2E2_4456 at 5189.380 kb on - strand, within Pf6N2E2_4456 at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.571 kb on - strand at 5189.630 kb on + strand, within Pf6N2E2_4457 at 5189.630 kb on + strand, within Pf6N2E2_4457 at 5189.631 kb on - strand, within Pf6N2E2_4457 at 5189.651 kb on + strand, within Pf6N2E2_4457 at 5189.652 kb on - strand, within Pf6N2E2_4457 at 5189.652 kb on - strand, within Pf6N2E2_4457 at 5189.673 kb on + strand, within Pf6N2E2_4457 at 5189.673 kb on + strand, within Pf6N2E2_4457 at 5189.674 kb on - strand, within Pf6N2E2_4457
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 5,187,350 + Pf6N2E2_4454 0.62 -0.6 5,187,350 + Pf6N2E2_4454 0.62 -1.1 5,187,350 + Pf6N2E2_4454 0.62 -1.1 5,187,351 - Pf6N2E2_4454 0.62 -0.5 5,187,351 - Pf6N2E2_4454 0.62 +2.4 5,187,351 - Pf6N2E2_4454 0.62 -0.5 5,187,352 + Pf6N2E2_4454 0.62 -0.3 5,187,352 + Pf6N2E2_4454 0.62 -1.8 5,187,352 + Pf6N2E2_4454 0.62 +1.4 5,187,353 - Pf6N2E2_4454 0.62 -0.1 5,187,353 - Pf6N2E2_4454 0.62 -0.1 5,187,398 - Pf6N2E2_4454 0.65 -1.0 5,187,409 + Pf6N2E2_4454 0.66 -0.5 5,187,410 - Pf6N2E2_4454 0.66 +1.5 5,187,692 + Pf6N2E2_4454 0.85 -1.5 5,187,693 - Pf6N2E2_4454 0.85 +0.7 5,188,109 + -0.7 5,188,109 + +0.6 5,188,180 + +0.1 5,188,180 + -1.1 5,188,591 + Pf6N2E2_4455 0.57 -0.2 5,188,591 + Pf6N2E2_4455 0.57 -0.5 5,188,591 + Pf6N2E2_4455 0.57 -0.9 5,188,592 - Pf6N2E2_4455 0.57 -0.5 5,188,592 - Pf6N2E2_4455 0.57 -2.5 5,188,712 + Pf6N2E2_4455 0.73 -0.2 5,188,712 + Pf6N2E2_4455 0.73 +0.2 5,188,713 - Pf6N2E2_4455 0.73 -0.1 5,188,872 - -0.2 5,188,872 - -0.4 5,188,874 + -0.0 5,188,874 + +1.4 5,188,874 + -0.2 5,188,874 + +1.5 5,188,875 - -0.3 5,188,875 - -0.1 5,188,875 - -0.3 5,188,875 - -0.3 5,188,875 - -0.4 5,188,875 - +1.3 5,188,878 + -0.3 5,188,879 - +0.6 5,188,879 - +0.7 5,188,879 - -0.7 5,188,879 - +0.4 5,188,896 + -0.1 5,188,896 + +0.7 5,188,896 + +0.3 5,188,897 - -1.7 5,188,897 - -0.9 5,188,897 - -0.5 5,188,897 - -0.3 5,188,965 + Pf6N2E2_4456 0.11 +0.1 5,188,965 + Pf6N2E2_4456 0.11 -0.8 5,188,966 - Pf6N2E2_4456 0.11 +0.3 5,188,986 + Pf6N2E2_4456 0.14 -0.8 5,188,987 - Pf6N2E2_4456 0.14 +0.0 5,188,987 - Pf6N2E2_4456 0.14 +0.8 5,188,987 - Pf6N2E2_4456 0.14 -1.3 5,189,149 - Pf6N2E2_4456 0.39 +0.9 5,189,248 - Pf6N2E2_4456 0.54 -0.3 5,189,258 - Pf6N2E2_4456 0.56 +0.9 5,189,258 - Pf6N2E2_4456 0.56 -0.5 5,189,379 + Pf6N2E2_4456 0.74 +0.4 5,189,379 + Pf6N2E2_4456 0.74 -0.5 5,189,379 + Pf6N2E2_4456 0.74 +0.4 5,189,379 + Pf6N2E2_4456 0.74 +1.1 5,189,379 + Pf6N2E2_4456 0.74 -1.1 5,189,380 - Pf6N2E2_4456 0.75 +0.3 5,189,380 - Pf6N2E2_4456 0.75 -0.6 5,189,570 + -0.2 5,189,570 + +0.8 5,189,570 + +1.0 5,189,570 + +0.3 5,189,571 - +0.6 5,189,630 + Pf6N2E2_4457 0.13 -1.0 5,189,630 + Pf6N2E2_4457 0.13 -1.5 5,189,631 - Pf6N2E2_4457 0.13 +1.1 5,189,651 + Pf6N2E2_4457 0.16 -0.6 5,189,652 - Pf6N2E2_4457 0.16 -0.0 5,189,652 - Pf6N2E2_4457 0.16 +0.5 5,189,673 + Pf6N2E2_4457 0.19 +0.2 5,189,673 + Pf6N2E2_4457 0.19 -0.3 5,189,674 - Pf6N2E2_4457 0.19 +0.4
Or see this region's nucleotide sequence