Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3832 and Pf6N2E2_3833 are separated by 177 nucleotides Pf6N2E2_3833 and Pf6N2E2_3834 overlap by 45 nucleotides Pf6N2E2_3834 and Pf6N2E2_3835 are separated by 32 nucleotides
Pf6N2E2_3832 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 4,465,261 to 4,466,760
_3832
Pf6N2E2_3833 - hypothetical protein, at 4,466,938 to 4,467,123
_3833
Pf6N2E2_3834 - Lipoprotein NlpD, at 4,467,079 to 4,468,371
_3834
Pf6N2E2_3835 - Carboxyl-terminal protease (EC 3.4.21.102), at 4,468,404 to 4,469,720
_3835
Position (kb)
4467
4468
4469 Strain fitness (log2 ratio)
-2
-1
0
1 at 4466.769 kb on + strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.770 kb on - strand at 4466.880 kb on + strand at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.042 kb on + strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.043 kb on - strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.045 kb on + strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.046 kb on - strand, within Pf6N2E2_3833 at 4467.078 kb on + strand, within Pf6N2E2_3833 at 4467.079 kb on - strand at 4467.079 kb on - strand at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.440 kb on - strand, within Pf6N2E2_3834 at 4467.496 kb on + strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.497 kb on - strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.499 kb on + strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4467.500 kb on - strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.016 kb on + strand, within Pf6N2E2_3834 at 4468.019 kb on + strand, within Pf6N2E2_3834 at 4468.020 kb on - strand, within Pf6N2E2_3834 at 4468.130 kb on + strand, within Pf6N2E2_3834 at 4468.131 kb on - strand, within Pf6N2E2_3834 at 4468.189 kb on + strand, within Pf6N2E2_3834 at 4468.190 kb on - strand, within Pf6N2E2_3834 at 4468.190 kb on - strand, within Pf6N2E2_3834 at 4468.293 kb on + strand at 4468.306 kb on + strand at 4468.306 kb on + strand at 4468.306 kb on + strand at 4468.369 kb on + strand at 4468.384 kb on + strand at 4468.384 kb on + strand at 4468.385 kb on - strand at 4468.385 kb on - strand at 4468.385 kb on - strand at 4468.590 kb on + strand, within Pf6N2E2_3835 at 4468.590 kb on + strand, within Pf6N2E2_3835 at 4468.591 kb on - strand, within Pf6N2E2_3835 at 4469.020 kb on - strand, within Pf6N2E2_3835 at 4469.205 kb on - strand, within Pf6N2E2_3835
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 4,466,769 + -0.5 4,466,770 - -1.9 4,466,770 - +0.5 4,466,770 - +1.0 4,466,770 - +0.3 4,466,880 + -1.8 4,467,042 + Pf6N2E2_3833 0.56 -0.4 4,467,042 + Pf6N2E2_3833 0.56 -1.8 4,467,043 - Pf6N2E2_3833 0.56 -1.0 4,467,043 - Pf6N2E2_3833 0.56 +0.7 4,467,045 + Pf6N2E2_3833 0.58 -0.5 4,467,045 + Pf6N2E2_3833 0.58 +0.1 4,467,046 - Pf6N2E2_3833 0.58 -0.1 4,467,046 - Pf6N2E2_3833 0.58 -1.4 4,467,046 - Pf6N2E2_3833 0.58 +0.1 4,467,046 - Pf6N2E2_3833 0.58 -1.8 4,467,046 - Pf6N2E2_3833 0.58 -1.3 4,467,078 + Pf6N2E2_3833 0.75 +0.3 4,467,079 - -0.4 4,467,079 - -2.2 4,467,440 - Pf6N2E2_3834 0.28 -1.4 4,467,440 - Pf6N2E2_3834 0.28 -0.8 4,467,496 + Pf6N2E2_3834 0.32 -0.1 4,467,497 - Pf6N2E2_3834 0.32 -0.3 4,467,497 - Pf6N2E2_3834 0.32 -0.7 4,467,499 + Pf6N2E2_3834 0.32 -2.2 4,467,499 + Pf6N2E2_3834 0.32 -2.2 4,467,500 - Pf6N2E2_3834 0.33 -0.5 4,467,500 - Pf6N2E2_3834 0.33 -0.2 4,468,016 + Pf6N2E2_3834 0.72 -1.1 4,468,016 + Pf6N2E2_3834 0.72 -0.8 4,468,016 + Pf6N2E2_3834 0.72 -2.3 4,468,016 + Pf6N2E2_3834 0.72 -2.2 4,468,019 + Pf6N2E2_3834 0.73 -1.8 4,468,020 - Pf6N2E2_3834 0.73 -0.8 4,468,130 + Pf6N2E2_3834 0.81 -1.6 4,468,131 - Pf6N2E2_3834 0.81 -0.3 4,468,189 + Pf6N2E2_3834 0.86 -0.3 4,468,190 - Pf6N2E2_3834 0.86 -1.1 4,468,190 - Pf6N2E2_3834 0.86 +0.3 4,468,293 + -2.4 4,468,306 + +0.1 4,468,306 + +1.0 4,468,306 + -2.4 4,468,369 + +0.3 4,468,384 + -1.2 4,468,384 + +0.6 4,468,385 - -0.6 4,468,385 - +0.4 4,468,385 - +0.1 4,468,590 + Pf6N2E2_3835 0.14 -0.1 4,468,590 + Pf6N2E2_3835 0.14 +1.3 4,468,591 - Pf6N2E2_3835 0.14 +1.2 4,469,020 - Pf6N2E2_3835 0.47 -0.5 4,469,205 - Pf6N2E2_3835 0.61 -1.2
Or see this region's nucleotide sequence