Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3794 and Pf6N2E2_3795 are separated by 105 nucleotides Pf6N2E2_3795 and Pf6N2E2_3796 are separated by 144 nucleotides
Pf6N2E2_3794 - Proline iminopeptidase (EC 3.4.11.5), at 4,420,107 to 4,421,078
_3794
Pf6N2E2_3795 - Lipolytic enzyme, at 4,421,184 to 4,422,095
_3795
Pf6N2E2_3796 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 4,422,240 to 4,423,187
_3796
Position (kb)
4421
4422
4423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4420.381 kb on + strand, within Pf6N2E2_3794 at 4420.381 kb on + strand, within Pf6N2E2_3794 at 4420.381 kb on + strand, within Pf6N2E2_3794 at 4420.382 kb on - strand, within Pf6N2E2_3794 at 4420.382 kb on - strand, within Pf6N2E2_3794 at 4420.382 kb on - strand, within Pf6N2E2_3794 at 4420.469 kb on + strand, within Pf6N2E2_3794 at 4420.469 kb on + strand, within Pf6N2E2_3794 at 4420.594 kb on + strand, within Pf6N2E2_3794 at 4420.594 kb on + strand, within Pf6N2E2_3794 at 4420.595 kb on - strand, within Pf6N2E2_3794 at 4420.595 kb on - strand, within Pf6N2E2_3794 at 4420.639 kb on + strand, within Pf6N2E2_3794 at 4420.639 kb on + strand, within Pf6N2E2_3794 at 4420.639 kb on + strand, within Pf6N2E2_3794 at 4420.640 kb on - strand, within Pf6N2E2_3794 at 4420.724 kb on - strand, within Pf6N2E2_3794 at 4420.915 kb on + strand, within Pf6N2E2_3794 at 4420.915 kb on + strand, within Pf6N2E2_3794 at 4420.915 kb on + strand, within Pf6N2E2_3794 at 4421.005 kb on + strand at 4421.006 kb on - strand at 4421.006 kb on - strand at 4421.121 kb on + strand at 4421.121 kb on + strand at 4421.121 kb on + strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.122 kb on - strand at 4421.125 kb on + strand at 4421.145 kb on + strand at 4421.145 kb on + strand at 4421.145 kb on + strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.146 kb on - strand at 4421.156 kb on + strand at 4421.157 kb on - strand at 4421.162 kb on - strand at 4421.221 kb on + strand at 4421.221 kb on + strand at 4421.222 kb on - strand at 4421.222 kb on - strand at 4421.222 kb on - strand at 4421.290 kb on + strand, within Pf6N2E2_3795 at 4421.290 kb on + strand, within Pf6N2E2_3795 at 4421.290 kb on + strand, within Pf6N2E2_3795 at 4421.290 kb on + strand, within Pf6N2E2_3795 at 4421.290 kb on + strand, within Pf6N2E2_3795 at 4421.291 kb on - strand, within Pf6N2E2_3795 at 4421.291 kb on - strand, within Pf6N2E2_3795 at 4421.291 kb on - strand, within Pf6N2E2_3795 at 4421.291 kb on - strand, within Pf6N2E2_3795 at 4421.333 kb on - strand, within Pf6N2E2_3795 at 4421.428 kb on + strand, within Pf6N2E2_3795 at 4421.428 kb on + strand, within Pf6N2E2_3795 at 4421.429 kb on - strand, within Pf6N2E2_3795 at 4421.429 kb on - strand, within Pf6N2E2_3795 at 4421.455 kb on + strand, within Pf6N2E2_3795 at 4421.610 kb on + strand, within Pf6N2E2_3795 at 4421.908 kb on + strand, within Pf6N2E2_3795 at 4421.908 kb on + strand, within Pf6N2E2_3795 at 4421.909 kb on - strand, within Pf6N2E2_3795 at 4422.088 kb on + strand at 4422.089 kb on - strand at 4422.466 kb on + strand, within Pf6N2E2_3796 at 4422.466 kb on + strand, within Pf6N2E2_3796 at 4422.467 kb on - strand, within Pf6N2E2_3796 at 4422.517 kb on + strand, within Pf6N2E2_3796 at 4422.517 kb on + strand, within Pf6N2E2_3796 at 4422.517 kb on + strand, within Pf6N2E2_3796 at 4422.518 kb on - strand, within Pf6N2E2_3796 at 4422.518 kb on - strand, within Pf6N2E2_3796 at 4422.518 kb on - strand, within Pf6N2E2_3796 at 4422.541 kb on + strand, within Pf6N2E2_3796 at 4422.541 kb on + strand, within Pf6N2E2_3796 at 4422.541 kb on + strand, within Pf6N2E2_3796 at 4422.541 kb on + strand, within Pf6N2E2_3796 at 4422.541 kb on + strand, within Pf6N2E2_3796 at 4422.745 kb on + strand, within Pf6N2E2_3796 at 4422.746 kb on - strand, within Pf6N2E2_3796 at 4422.746 kb on - strand, within Pf6N2E2_3796 at 4422.746 kb on - strand, within Pf6N2E2_3796 at 4422.817 kb on + strand, within Pf6N2E2_3796 at 4422.817 kb on + strand, within Pf6N2E2_3796 at 4422.817 kb on + strand, within Pf6N2E2_3796 at 4422.817 kb on + strand, within Pf6N2E2_3796 at 4422.870 kb on + strand, within Pf6N2E2_3796 at 4422.871 kb on - strand, within Pf6N2E2_3796 at 4422.871 kb on - strand, within Pf6N2E2_3796 at 4422.931 kb on + strand, within Pf6N2E2_3796
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 4,420,381 + Pf6N2E2_3794 0.28 +0.5 4,420,381 + Pf6N2E2_3794 0.28 -2.4 4,420,381 + Pf6N2E2_3794 0.28 -1.6 4,420,382 - Pf6N2E2_3794 0.28 +0.3 4,420,382 - Pf6N2E2_3794 0.28 -0.1 4,420,382 - Pf6N2E2_3794 0.28 +0.5 4,420,469 + Pf6N2E2_3794 0.37 -0.5 4,420,469 + Pf6N2E2_3794 0.37 -0.6 4,420,594 + Pf6N2E2_3794 0.50 +0.1 4,420,594 + Pf6N2E2_3794 0.50 -0.5 4,420,595 - Pf6N2E2_3794 0.50 -0.4 4,420,595 - Pf6N2E2_3794 0.50 +1.5 4,420,639 + Pf6N2E2_3794 0.55 +0.8 4,420,639 + Pf6N2E2_3794 0.55 -1.0 4,420,639 + Pf6N2E2_3794 0.55 -0.7 4,420,640 - Pf6N2E2_3794 0.55 -0.0 4,420,724 - Pf6N2E2_3794 0.63 +0.6 4,420,915 + Pf6N2E2_3794 0.83 -0.6 4,420,915 + Pf6N2E2_3794 0.83 +0.8 4,420,915 + Pf6N2E2_3794 0.83 -0.8 4,421,005 + -0.7 4,421,006 - -0.5 4,421,006 - +0.3 4,421,121 + -3.0 4,421,121 + +0.1 4,421,121 + +0.8 4,421,122 - -1.5 4,421,122 - +1.1 4,421,122 - -0.4 4,421,122 - -0.3 4,421,122 - -0.5 4,421,122 - -2.0 4,421,122 - -1.0 4,421,125 + -0.4 4,421,145 + -1.1 4,421,145 + +1.1 4,421,145 + +0.2 4,421,146 - -1.1 4,421,146 - +0.7 4,421,146 - +0.5 4,421,146 - +0.8 4,421,146 - -0.9 4,421,146 - -0.1 4,421,146 - +0.4 4,421,146 - +0.3 4,421,156 + +0.5 4,421,157 - +0.8 4,421,162 - +0.3 4,421,221 + +2.2 4,421,221 + +0.4 4,421,222 - -0.2 4,421,222 - -1.7 4,421,222 - +0.2 4,421,290 + Pf6N2E2_3795 0.12 -0.7 4,421,290 + Pf6N2E2_3795 0.12 -2.4 4,421,290 + Pf6N2E2_3795 0.12 +0.6 4,421,290 + Pf6N2E2_3795 0.12 +0.1 4,421,290 + Pf6N2E2_3795 0.12 +0.1 4,421,291 - Pf6N2E2_3795 0.12 -0.0 4,421,291 - Pf6N2E2_3795 0.12 +1.1 4,421,291 - Pf6N2E2_3795 0.12 +0.5 4,421,291 - Pf6N2E2_3795 0.12 -0.9 4,421,333 - Pf6N2E2_3795 0.16 +1.6 4,421,428 + Pf6N2E2_3795 0.27 -1.8 4,421,428 + Pf6N2E2_3795 0.27 -1.1 4,421,429 - Pf6N2E2_3795 0.27 -0.4 4,421,429 - Pf6N2E2_3795 0.27 +0.1 4,421,455 + Pf6N2E2_3795 0.30 -0.3 4,421,610 + Pf6N2E2_3795 0.47 -0.2 4,421,908 + Pf6N2E2_3795 0.79 +0.4 4,421,908 + Pf6N2E2_3795 0.79 +0.2 4,421,909 - Pf6N2E2_3795 0.79 -0.5 4,422,088 + +0.9 4,422,089 - +0.0 4,422,466 + Pf6N2E2_3796 0.24 -0.5 4,422,466 + Pf6N2E2_3796 0.24 +1.0 4,422,467 - Pf6N2E2_3796 0.24 +0.5 4,422,517 + Pf6N2E2_3796 0.29 +0.9 4,422,517 + Pf6N2E2_3796 0.29 +0.2 4,422,517 + Pf6N2E2_3796 0.29 -0.1 4,422,518 - Pf6N2E2_3796 0.29 -0.2 4,422,518 - Pf6N2E2_3796 0.29 +1.5 4,422,518 - Pf6N2E2_3796 0.29 -0.6 4,422,541 + Pf6N2E2_3796 0.32 +0.2 4,422,541 + Pf6N2E2_3796 0.32 -0.0 4,422,541 + Pf6N2E2_3796 0.32 -0.3 4,422,541 + Pf6N2E2_3796 0.32 -0.8 4,422,541 + Pf6N2E2_3796 0.32 -0.9 4,422,745 + Pf6N2E2_3796 0.53 +0.5 4,422,746 - Pf6N2E2_3796 0.53 +0.5 4,422,746 - Pf6N2E2_3796 0.53 +0.4 4,422,746 - Pf6N2E2_3796 0.53 +0.4 4,422,817 + Pf6N2E2_3796 0.61 +0.5 4,422,817 + Pf6N2E2_3796 0.61 -1.4 4,422,817 + Pf6N2E2_3796 0.61 +0.5 4,422,817 + Pf6N2E2_3796 0.61 -1.6 4,422,870 + Pf6N2E2_3796 0.66 -0.1 4,422,871 - Pf6N2E2_3796 0.67 +0.4 4,422,871 - Pf6N2E2_3796 0.67 +1.5 4,422,931 + Pf6N2E2_3796 0.73 +2.5
Or see this region's nucleotide sequence