Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2043 and Pf6N2E2_2044 are separated by 60 nucleotides Pf6N2E2_2044 and Pf6N2E2_2045 are separated by 48 nucleotides Pf6N2E2_2045 and Pf6N2E2_2046 are separated by 198 nucleotides
Pf6N2E2_2043 - Twitching motility protein PilT, at 2,433,552 to 2,434,739
_2043
Pf6N2E2_2044 - Histidine kinase/response regulator hybrid protein, at 2,434,800 to 2,437,337
_2044
Pf6N2E2_2045 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 2,437,386 to 2,437,661
_2045
Pf6N2E2_2046 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 2,437,860 to 2,438,777
_2046
Position (kb)
2434
2435
2436
2437
2438 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2434.176 kb on - strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.320 kb on + strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.605 kb on - strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.612 kb on + strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.613 kb on - strand, within Pf6N2E2_2043 at 2434.741 kb on + strand at 2434.742 kb on - strand at 2434.742 kb on - strand at 2434.757 kb on + strand at 2434.757 kb on + strand at 2434.758 kb on - strand at 2434.760 kb on + strand at 2434.761 kb on - strand at 2434.761 kb on - strand at 2434.826 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.828 kb on + strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.829 kb on - strand at 2434.977 kb on + strand at 2435.042 kb on + strand at 2435.043 kb on - strand at 2435.043 kb on - strand at 2435.066 kb on + strand, within Pf6N2E2_2044 at 2435.066 kb on + strand, within Pf6N2E2_2044 at 2435.082 kb on + strand, within Pf6N2E2_2044 at 2435.082 kb on + strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.083 kb on - strand, within Pf6N2E2_2044 at 2435.138 kb on - strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.181 kb on + strand, within Pf6N2E2_2044 at 2435.182 kb on - strand, within Pf6N2E2_2044 at 2435.394 kb on - strand, within Pf6N2E2_2044 at 2435.457 kb on - strand, within Pf6N2E2_2044 at 2435.457 kb on - strand, within Pf6N2E2_2044 at 2435.556 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.595 kb on + strand, within Pf6N2E2_2044 at 2435.596 kb on - strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.613 kb on + strand, within Pf6N2E2_2044 at 2435.614 kb on - strand, within Pf6N2E2_2044 at 2435.754 kb on + strand, within Pf6N2E2_2044 at 2435.755 kb on - strand, within Pf6N2E2_2044 at 2435.755 kb on - strand, within Pf6N2E2_2044 at 2435.796 kb on + strand, within Pf6N2E2_2044 at 2435.797 kb on - strand, within Pf6N2E2_2044 at 2435.809 kb on - strand, within Pf6N2E2_2044 at 2435.871 kb on + strand, within Pf6N2E2_2044 at 2435.871 kb on + strand, within Pf6N2E2_2044 at 2435.979 kb on + strand, within Pf6N2E2_2044 at 2435.979 kb on + strand, within Pf6N2E2_2044 at 2435.979 kb on + strand, within Pf6N2E2_2044 at 2435.979 kb on + strand, within Pf6N2E2_2044 at 2435.980 kb on - strand, within Pf6N2E2_2044 at 2435.980 kb on - strand, within Pf6N2E2_2044 at 2436.021 kb on + strand, within Pf6N2E2_2044 at 2436.022 kb on - strand, within Pf6N2E2_2044 at 2436.022 kb on - strand, within Pf6N2E2_2044 at 2436.022 kb on - strand, within Pf6N2E2_2044 at 2436.022 kb on - strand, within Pf6N2E2_2044 at 2436.297 kb on + strand, within Pf6N2E2_2044 at 2436.297 kb on + strand, within Pf6N2E2_2044 at 2436.298 kb on - strand, within Pf6N2E2_2044 at 2436.298 kb on - strand, within Pf6N2E2_2044 at 2436.639 kb on + strand, within Pf6N2E2_2044 at 2436.640 kb on - strand, within Pf6N2E2_2044 at 2436.690 kb on + strand, within Pf6N2E2_2044 at 2436.690 kb on + strand, within Pf6N2E2_2044 at 2436.691 kb on - strand, within Pf6N2E2_2044 at 2436.892 kb on - strand, within Pf6N2E2_2044 at 2436.892 kb on - strand, within Pf6N2E2_2044 at 2437.347 kb on + strand at 2437.347 kb on + strand at 2437.348 kb on - strand at 2437.348 kb on - strand at 2437.348 kb on - strand at 2437.492 kb on - strand, within Pf6N2E2_2045 at 2437.537 kb on + strand, within Pf6N2E2_2045 at 2437.538 kb on - strand, within Pf6N2E2_2045 at 2437.651 kb on - strand at 2437.970 kb on - strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.041 kb on + strand, within Pf6N2E2_2046 at 2438.042 kb on - strand, within Pf6N2E2_2046 at 2438.042 kb on - strand, within Pf6N2E2_2046 at 2438.092 kb on - strand, within Pf6N2E2_2046 at 2438.120 kb on - strand, within Pf6N2E2_2046 at 2438.120 kb on - strand, within Pf6N2E2_2046 at 2438.176 kb on - strand, within Pf6N2E2_2046 at 2438.200 kb on + strand, within Pf6N2E2_2046 at 2438.219 kb on - strand, within Pf6N2E2_2046
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 2,434,176 - Pf6N2E2_2043 0.53 -1.0 2,434,177 + Pf6N2E2_2043 0.53 +0.7 2,434,177 + Pf6N2E2_2043 0.53 -1.7 2,434,177 + Pf6N2E2_2043 0.53 +1.2 2,434,177 + Pf6N2E2_2043 0.53 -0.2 2,434,178 - Pf6N2E2_2043 0.53 -0.1 2,434,178 - Pf6N2E2_2043 0.53 +1.7 2,434,178 - Pf6N2E2_2043 0.53 +0.9 2,434,320 + Pf6N2E2_2043 0.65 -0.3 2,434,605 - Pf6N2E2_2043 0.89 -0.4 2,434,605 - Pf6N2E2_2043 0.89 +1.5 2,434,605 - Pf6N2E2_2043 0.89 +0.4 2,434,612 + Pf6N2E2_2043 0.89 -0.5 2,434,612 + Pf6N2E2_2043 0.89 +0.3 2,434,612 + Pf6N2E2_2043 0.89 +0.0 2,434,612 + Pf6N2E2_2043 0.89 +0.4 2,434,613 - Pf6N2E2_2043 0.89 -0.2 2,434,613 - Pf6N2E2_2043 0.89 +0.5 2,434,613 - Pf6N2E2_2043 0.89 +0.0 2,434,613 - Pf6N2E2_2043 0.89 +0.1 2,434,741 + -0.5 2,434,742 - +0.9 2,434,742 - +0.8 2,434,757 + -1.1 2,434,757 + +1.1 2,434,758 - -0.5 2,434,760 + -1.5 2,434,761 - -0.1 2,434,761 - -0.7 2,434,826 + -1.4 2,434,828 + +0.8 2,434,828 + -0.3 2,434,828 + -1.0 2,434,828 + +0.5 2,434,828 + +0.4 2,434,828 + +0.6 2,434,828 + +1.9 2,434,828 + +0.1 2,434,828 + +1.1 2,434,829 - +1.0 2,434,829 - -0.7 2,434,829 - -1.0 2,434,829 - +1.5 2,434,829 - +0.2 2,434,829 - +0.9 2,434,829 - +0.6 2,434,829 - +0.5 2,434,977 + +0.5 2,435,042 + +0.3 2,435,043 - +0.9 2,435,043 - -0.8 2,435,066 + Pf6N2E2_2044 0.10 -0.5 2,435,066 + Pf6N2E2_2044 0.10 -0.6 2,435,082 + Pf6N2E2_2044 0.11 +0.5 2,435,082 + Pf6N2E2_2044 0.11 -0.0 2,435,083 - Pf6N2E2_2044 0.11 -0.1 2,435,083 - Pf6N2E2_2044 0.11 +0.3 2,435,083 - Pf6N2E2_2044 0.11 -0.7 2,435,083 - Pf6N2E2_2044 0.11 +1.3 2,435,083 - Pf6N2E2_2044 0.11 +0.5 2,435,138 - Pf6N2E2_2044 0.13 +0.7 2,435,181 + Pf6N2E2_2044 0.15 +0.5 2,435,181 + Pf6N2E2_2044 0.15 +0.4 2,435,181 + Pf6N2E2_2044 0.15 -0.5 2,435,181 + Pf6N2E2_2044 0.15 +0.3 2,435,181 + Pf6N2E2_2044 0.15 -0.2 2,435,181 + Pf6N2E2_2044 0.15 -1.0 2,435,182 - Pf6N2E2_2044 0.15 +0.1 2,435,394 - Pf6N2E2_2044 0.23 -0.3 2,435,457 - Pf6N2E2_2044 0.26 +0.5 2,435,457 - Pf6N2E2_2044 0.26 +0.5 2,435,556 + Pf6N2E2_2044 0.30 +1.2 2,435,595 + Pf6N2E2_2044 0.31 -1.8 2,435,595 + Pf6N2E2_2044 0.31 -0.9 2,435,595 + Pf6N2E2_2044 0.31 -1.3 2,435,596 - Pf6N2E2_2044 0.31 +0.5 2,435,613 + Pf6N2E2_2044 0.32 -0.5 2,435,613 + Pf6N2E2_2044 0.32 +0.3 2,435,613 + Pf6N2E2_2044 0.32 +0.1 2,435,614 - Pf6N2E2_2044 0.32 -0.8 2,435,754 + Pf6N2E2_2044 0.38 -0.8 2,435,755 - Pf6N2E2_2044 0.38 +0.5 2,435,755 - Pf6N2E2_2044 0.38 +0.2 2,435,796 + Pf6N2E2_2044 0.39 -1.0 2,435,797 - Pf6N2E2_2044 0.39 -0.6 2,435,809 - Pf6N2E2_2044 0.40 +0.2 2,435,871 + Pf6N2E2_2044 0.42 +0.2 2,435,871 + Pf6N2E2_2044 0.42 -0.7 2,435,979 + Pf6N2E2_2044 0.46 +0.4 2,435,979 + Pf6N2E2_2044 0.46 +1.5 2,435,979 + Pf6N2E2_2044 0.46 +2.3 2,435,979 + Pf6N2E2_2044 0.46 -0.2 2,435,980 - Pf6N2E2_2044 0.46 -0.3 2,435,980 - Pf6N2E2_2044 0.46 -0.6 2,436,021 + Pf6N2E2_2044 0.48 +0.3 2,436,022 - Pf6N2E2_2044 0.48 -0.9 2,436,022 - Pf6N2E2_2044 0.48 +1.1 2,436,022 - Pf6N2E2_2044 0.48 +0.2 2,436,022 - Pf6N2E2_2044 0.48 +1.1 2,436,297 + Pf6N2E2_2044 0.59 +0.0 2,436,297 + Pf6N2E2_2044 0.59 -0.4 2,436,298 - Pf6N2E2_2044 0.59 -1.0 2,436,298 - Pf6N2E2_2044 0.59 +0.0 2,436,639 + Pf6N2E2_2044 0.72 +0.1 2,436,640 - Pf6N2E2_2044 0.72 +1.2 2,436,690 + Pf6N2E2_2044 0.74 +0.3 2,436,690 + Pf6N2E2_2044 0.74 +0.5 2,436,691 - Pf6N2E2_2044 0.75 -0.4 2,436,892 - Pf6N2E2_2044 0.82 +0.5 2,436,892 - Pf6N2E2_2044 0.82 -0.9 2,437,347 + +0.6 2,437,347 + +0.1 2,437,348 - -2.7 2,437,348 - +1.5 2,437,348 - -0.4 2,437,492 - Pf6N2E2_2045 0.38 +0.7 2,437,537 + Pf6N2E2_2045 0.55 +0.3 2,437,538 - Pf6N2E2_2045 0.55 -0.6 2,437,651 - +1.1 2,437,970 - Pf6N2E2_2046 0.12 +0.0 2,438,041 + Pf6N2E2_2046 0.20 +0.5 2,438,041 + Pf6N2E2_2046 0.20 -0.5 2,438,041 + Pf6N2E2_2046 0.20 -0.3 2,438,042 - Pf6N2E2_2046 0.20 -0.3 2,438,042 - Pf6N2E2_2046 0.20 +0.1 2,438,092 - Pf6N2E2_2046 0.25 +0.7 2,438,120 - Pf6N2E2_2046 0.28 +1.0 2,438,120 - Pf6N2E2_2046 0.28 -1.8 2,438,176 - Pf6N2E2_2046 0.34 -0.8 2,438,200 + Pf6N2E2_2046 0.37 -1.7 2,438,219 - Pf6N2E2_2046 0.39 +0.2
Or see this region's nucleotide sequence