Experiment: Hydroxide 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1174 and Pf6N2E2_1175 are separated by 269 nucleotides Pf6N2E2_1175 and Pf6N2E2_1176 are separated by 73 nucleotides Pf6N2E2_1176 and Pf6N2E2_1177 are separated by 12 nucleotides
Pf6N2E2_1174 - hypothetical protein, at 1,373,964 to 1,375,022
_1174
Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517
_1175
Pf6N2E2_1176 - Mll2356 protein, at 1,377,591 to 1,377,986
_1176
Pf6N2E2_1177 - putative exported protein, at 1,377,999 to 1,379,141
_1177
Position (kb)
1375
1376
1377
1378 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1374.337 kb on + strand, within Pf6N2E2_1174 at 1374.456 kb on + strand, within Pf6N2E2_1174 at 1374.457 kb on - strand, within Pf6N2E2_1174 at 1374.469 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.488 kb on + strand, within Pf6N2E2_1174 at 1374.595 kb on - strand, within Pf6N2E2_1174 at 1374.681 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.711 kb on - strand, within Pf6N2E2_1174 at 1374.772 kb on - strand, within Pf6N2E2_1174 at 1374.794 kb on + strand, within Pf6N2E2_1174 at 1374.816 kb on + strand, within Pf6N2E2_1174 at 1374.817 kb on - strand, within Pf6N2E2_1174 at 1374.817 kb on - strand, within Pf6N2E2_1174 at 1374.861 kb on + strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.862 kb on - strand, within Pf6N2E2_1174 at 1374.946 kb on + strand at 1374.946 kb on + strand at 1374.947 kb on - strand at 1374.996 kb on + strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1374.997 kb on - strand at 1375.056 kb on - strand at 1375.108 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.136 kb on + strand at 1375.167 kb on + strand at 1375.207 kb on - strand at 1375.207 kb on - strand at 1375.207 kb on - strand at 1375.281 kb on + strand at 1375.487 kb on + strand at 1375.487 kb on + strand at 1375.487 kb on + strand at 1375.727 kb on + strand, within Pf6N2E2_1175 at 1375.727 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.793 kb on + strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.794 kb on - strand, within Pf6N2E2_1175 at 1375.796 kb on + strand, within Pf6N2E2_1175 at 1375.848 kb on - strand, within Pf6N2E2_1175 at 1375.874 kb on + strand, within Pf6N2E2_1175 at 1375.875 kb on - strand, within Pf6N2E2_1175 at 1375.932 kb on - strand, within Pf6N2E2_1175 at 1375.932 kb on - strand, within Pf6N2E2_1175 at 1376.066 kb on + strand, within Pf6N2E2_1175 at 1376.084 kb on + strand, within Pf6N2E2_1175 at 1376.084 kb on + strand, within Pf6N2E2_1175 at 1376.112 kb on - strand, within Pf6N2E2_1175 at 1376.120 kb on - strand, within Pf6N2E2_1175 at 1376.216 kb on + strand, within Pf6N2E2_1175 at 1376.244 kb on - strand, within Pf6N2E2_1175 at 1376.244 kb on - strand, within Pf6N2E2_1175 at 1376.247 kb on + strand, within Pf6N2E2_1175 at 1376.247 kb on + strand, within Pf6N2E2_1175 at 1376.247 kb on + strand, within Pf6N2E2_1175 at 1376.247 kb on + strand, within Pf6N2E2_1175 at 1376.247 kb on + strand, within Pf6N2E2_1175 at 1376.248 kb on - strand, within Pf6N2E2_1175 at 1376.248 kb on - strand, within Pf6N2E2_1175 at 1376.286 kb on - strand, within Pf6N2E2_1175 at 1377.197 kb on - strand, within Pf6N2E2_1175 at 1377.395 kb on - strand at 1377.395 kb on - strand at 1377.515 kb on + strand at 1377.515 kb on + strand at 1377.534 kb on - strand at 1377.546 kb on + strand at 1377.683 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.973 kb on + strand at 1378.037 kb on + strand at 1378.037 kb on + strand at 1378.038 kb on - strand at 1378.096 kb on - strand at 1378.169 kb on - strand, within Pf6N2E2_1177 at 1378.204 kb on - strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxide 0.05 M remove 1,374,337 + Pf6N2E2_1174 0.35 +0.5 1,374,456 + Pf6N2E2_1174 0.46 +0.3 1,374,457 - Pf6N2E2_1174 0.47 +0.1 1,374,469 + Pf6N2E2_1174 0.48 +2.3 1,374,488 + Pf6N2E2_1174 0.49 +0.8 1,374,488 + Pf6N2E2_1174 0.49 +0.6 1,374,595 - Pf6N2E2_1174 0.60 +0.5 1,374,681 - Pf6N2E2_1174 0.68 +1.0 1,374,711 - Pf6N2E2_1174 0.71 +0.1 1,374,711 - Pf6N2E2_1174 0.71 -0.2 1,374,711 - Pf6N2E2_1174 0.71 +0.6 1,374,711 - Pf6N2E2_1174 0.71 -0.9 1,374,772 - Pf6N2E2_1174 0.76 -0.1 1,374,794 + Pf6N2E2_1174 0.78 -0.4 1,374,816 + Pf6N2E2_1174 0.80 +0.9 1,374,817 - Pf6N2E2_1174 0.81 +0.3 1,374,817 - Pf6N2E2_1174 0.81 +1.0 1,374,861 + Pf6N2E2_1174 0.85 +0.1 1,374,862 - Pf6N2E2_1174 0.85 +1.5 1,374,862 - Pf6N2E2_1174 0.85 +0.3 1,374,862 - Pf6N2E2_1174 0.85 -1.6 1,374,946 + +0.5 1,374,946 + +1.5 1,374,947 - -0.8 1,374,996 + +1.8 1,374,997 - +0.3 1,374,997 - -0.6 1,374,997 - +0.0 1,374,997 - -0.7 1,375,056 - -0.2 1,375,108 + +0.6 1,375,136 + -0.4 1,375,136 + +0.6 1,375,136 + -1.5 1,375,136 + +0.0 1,375,167 + -0.0 1,375,207 - +1.9 1,375,207 - +1.9 1,375,207 - +1.8 1,375,281 + +0.7 1,375,487 + +1.2 1,375,487 + -0.7 1,375,487 + -0.3 1,375,727 + Pf6N2E2_1175 0.20 -0.8 1,375,727 + Pf6N2E2_1175 0.20 -1.6 1,375,793 + Pf6N2E2_1175 0.23 +0.3 1,375,793 + Pf6N2E2_1175 0.23 -0.3 1,375,793 + Pf6N2E2_1175 0.23 -1.0 1,375,794 - Pf6N2E2_1175 0.23 +0.4 1,375,794 - Pf6N2E2_1175 0.23 -0.4 1,375,794 - Pf6N2E2_1175 0.23 -0.2 1,375,794 - Pf6N2E2_1175 0.23 -0.6 1,375,796 + Pf6N2E2_1175 0.23 -0.9 1,375,848 - Pf6N2E2_1175 0.25 -0.4 1,375,874 + Pf6N2E2_1175 0.26 +1.7 1,375,875 - Pf6N2E2_1175 0.26 +1.4 1,375,932 - Pf6N2E2_1175 0.29 +1.3 1,375,932 - Pf6N2E2_1175 0.29 -0.3 1,376,066 + Pf6N2E2_1175 0.35 -0.3 1,376,084 + Pf6N2E2_1175 0.36 -0.1 1,376,084 + Pf6N2E2_1175 0.36 +0.7 1,376,112 - Pf6N2E2_1175 0.37 +0.1 1,376,120 - Pf6N2E2_1175 0.37 -1.0 1,376,216 + Pf6N2E2_1175 0.42 -0.6 1,376,244 - Pf6N2E2_1175 0.43 -0.5 1,376,244 - Pf6N2E2_1175 0.43 +2.2 1,376,247 + Pf6N2E2_1175 0.43 -1.0 1,376,247 + Pf6N2E2_1175 0.43 +0.5 1,376,247 + Pf6N2E2_1175 0.43 -0.5 1,376,247 + Pf6N2E2_1175 0.43 -0.6 1,376,247 + Pf6N2E2_1175 0.43 -1.3 1,376,248 - Pf6N2E2_1175 0.43 -0.7 1,376,248 - Pf6N2E2_1175 0.43 +0.2 1,376,286 - Pf6N2E2_1175 0.45 +0.7 1,377,197 - Pf6N2E2_1175 0.86 +0.2 1,377,395 - +1.7 1,377,395 - -0.5 1,377,515 + -1.5 1,377,515 + +1.2 1,377,534 - +0.7 1,377,546 + +0.1 1,377,683 + Pf6N2E2_1176 0.23 -2.4 1,377,907 + Pf6N2E2_1176 0.80 +0.1 1,377,907 + Pf6N2E2_1176 0.80 -0.7 1,377,908 - Pf6N2E2_1176 0.80 +1.3 1,377,908 - Pf6N2E2_1176 0.80 +0.3 1,377,908 - Pf6N2E2_1176 0.80 +1.3 1,377,908 - Pf6N2E2_1176 0.80 +0.7 1,377,973 + +0.8 1,378,037 + +1.6 1,378,037 + +0.3 1,378,038 - +0.5 1,378,096 - +0.6 1,378,169 - Pf6N2E2_1177 0.15 -0.3 1,378,204 - Pf6N2E2_1177 0.18 -0.7 1,378,356 + Pf6N2E2_1177 0.31 -0.5 1,378,356 + Pf6N2E2_1177 0.31 -1.3
Or see this region's nucleotide sequence