Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4183 and Dshi_4184 are separated by 154 nucleotides Dshi_4184 and Dshi_4185 are separated by 251 nucleotides Dshi_4185 and Dshi_4186 are separated by 87 nucleotides Dshi_4186 and Dshi_4187 overlap by 4 nucleotides
Dshi_4183: Dshi_4183 - hypothetical protein (RefSeq), at 30,634 to 30,933
_4183
Dshi_4184: Dshi_4184 - CsbD family protein (RefSeq), at 31,088 to 31,285
_4184
Dshi_4185: Dshi_4185 - GntR domain protein (RefSeq), at 31,537 to 32,229
_4185
Dshi_4186: Dshi_4186 - ABC transporter related (RefSeq), at 32,317 to 33,138
_4186
Dshi_4187: Dshi_4187 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 33,135 to 33,890
_4187
Position (kb) 31
32
33 Strain fitness (log2 ratio) -1
0
1
2 at 30.608 kb on + strand at 30.659 kb on - strand at 30.803 kb on - strand, within Dshi_4183 at 30.987 kb on - strand at 31.094 kb on - strand at 31.094 kb on - strand at 31.103 kb on + strand at 31.164 kb on + strand, within Dshi_4184 at 31.427 kb on + strand at 31.446 kb on + strand at 31.502 kb on - strand at 31.678 kb on + strand, within Dshi_4185 at 31.847 kb on - strand, within Dshi_4185 at 32.014 kb on + strand, within Dshi_4185 at 32.066 kb on - strand, within Dshi_4185 at 32.389 kb on - strand at 32.431 kb on - strand, within Dshi_4186 at 32.582 kb on + strand, within Dshi_4186 at 32.724 kb on - strand, within Dshi_4186 at 32.954 kb on - strand, within Dshi_4186 at 33.036 kb on - strand, within Dshi_4186 at 33.081 kb on + strand at 33.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 30,608 + -0.9 30,659 - +1.4 30,803 - Dshi_4183 0.56 +1.8 30,987 - +0.1 31,094 - -0.2 31,094 - -0.4 31,103 + -0.7 31,164 + Dshi_4184 0.38 +1.1 31,427 + -0.7 31,446 + +0.8 31,502 - +0.3 31,678 + Dshi_4185 0.20 -0.9 31,847 - Dshi_4185 0.45 -0.4 32,014 + Dshi_4185 0.69 -0.4 32,066 - Dshi_4185 0.76 +2.1 32,389 - +0.1 32,431 - Dshi_4186 0.14 -0.0 32,582 + Dshi_4186 0.32 +1.0 32,724 - Dshi_4186 0.50 -0.9 32,954 - Dshi_4186 0.77 +2.1 33,036 - Dshi_4186 0.87 -1.3 33,081 + +0.7 33,148 + -1.2
Or see this region's nucleotide sequence