Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3040 and Dshi_3041 are separated by 118 nucleotides Dshi_3041 and Dshi_3042 are separated by 3 nucleotides Dshi_3042 and Dshi_3043 overlap by 1 nucleotides
Dshi_3040: Dshi_3040 - Dihydrodipicolinate reductase (RefSeq), at 3,209,163 to 3,209,972
_3040
Dshi_3041: Dshi_3041 - ribosome-binding factor A (RefSeq), at 3,210,091 to 3,210,498
_3041
Dshi_3042: Dshi_3042 - tRNA pseudouridine synthase B (RefSeq), at 3,210,502 to 3,211,416
_3042
Dshi_3043: Dshi_3043 - protein of unknown function DUF1294 (RefSeq), at 3,211,416 to 3,211,694
_3043
Position (kb)
3210
3211 Strain fitness (log2 ratio)
-1
0
1 at 3209.103 kb on - strand at 3209.990 kb on - strand at 3210.090 kb on - strand at 3210.251 kb on + strand, within Dshi_3041 at 3210.275 kb on + strand, within Dshi_3041 at 3210.375 kb on - strand, within Dshi_3041 at 3210.492 kb on + strand at 3210.499 kb on + strand at 3210.537 kb on - strand at 3210.580 kb on + strand at 3210.599 kb on - strand, within Dshi_3042 at 3210.766 kb on - strand, within Dshi_3042 at 3210.873 kb on + strand, within Dshi_3042 at 3211.017 kb on + strand, within Dshi_3042 at 3211.112 kb on + strand, within Dshi_3042 at 3211.182 kb on + strand, within Dshi_3042 at 3211.343 kb on - strand at 3211.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 3,209,103 - -0.7 3,209,990 - -0.4 3,210,090 - -0.8 3,210,251 + Dshi_3041 0.39 -0.1 3,210,275 + Dshi_3041 0.45 -0.5 3,210,375 - Dshi_3041 0.70 -0.9 3,210,492 + -0.2 3,210,499 + -0.0 3,210,537 - +0.0 3,210,580 + +0.2 3,210,599 - Dshi_3042 0.11 -0.0 3,210,766 - Dshi_3042 0.29 +0.4 3,210,873 + Dshi_3042 0.41 -0.6 3,211,017 + Dshi_3042 0.56 -0.2 3,211,112 + Dshi_3042 0.67 -0.1 3,211,182 + Dshi_3042 0.74 +0.4 3,211,343 - -0.5 3,211,385 - -0.9
Or see this region's nucleotide sequence