Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2583 and Dshi_2584 are separated by 324 nucleotides Dshi_2584 and Dshi_2585 are separated by 43 nucleotides Dshi_2585 and Dshi_2586 are separated by 18 nucleotides
Dshi_2583: Dshi_2583 - ribosomal protein L28 (RefSeq), at 2,749,939 to 2,750,226
_2583
Dshi_2584: Dshi_2584 - hypothetical protein (RefSeq), at 2,750,551 to 2,750,907
_2584
Dshi_2585: Dshi_2585 - protein of unknown function DUF461 (RefSeq), at 2,750,951 to 2,751,463
_2585
Dshi_2586: Dshi_2586 - HpcH/HpaI aldolase (RefSeq), at 2,751,482 to 2,752,237
_2586
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-1
0
1 at 2750.616 kb on + strand, within Dshi_2584 at 2750.858 kb on - strand, within Dshi_2584 at 2750.930 kb on + strand at 2750.935 kb on + strand at 2751.220 kb on - strand, within Dshi_2585 at 2751.254 kb on + strand, within Dshi_2585 at 2751.344 kb on - strand, within Dshi_2585 at 2751.379 kb on + strand, within Dshi_2585 at 2751.421 kb on - strand at 2751.629 kb on + strand, within Dshi_2586 at 2751.648 kb on + strand, within Dshi_2586 at 2751.833 kb on + strand, within Dshi_2586 at 2752.255 kb on - strand at 2752.350 kb on - strand at 2752.444 kb on + strand at 2752.444 kb on + strand at 2752.452 kb on - strand at 2752.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 2,750,616 + Dshi_2584 0.18 +0.3 2,750,858 - Dshi_2584 0.86 +1.2 2,750,930 + +0.8 2,750,935 + +0.0 2,751,220 - Dshi_2585 0.52 -0.7 2,751,254 + Dshi_2585 0.59 -0.3 2,751,344 - Dshi_2585 0.77 -1.1 2,751,379 + Dshi_2585 0.83 +0.1 2,751,421 - -0.6 2,751,629 + Dshi_2586 0.19 +0.7 2,751,648 + Dshi_2586 0.22 +0.4 2,751,833 + Dshi_2586 0.46 -0.1 2,752,255 - -0.3 2,752,350 - -0.8 2,752,444 + -0.3 2,752,444 + +0.7 2,752,452 - -0.3 2,752,452 - -0.4
Or see this region's nucleotide sequence