Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4921

Experiment: Vanillyl Alcohol 0.005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4919 and Pf6N2E2_4920 are separated by 46 nucleotidesPf6N2E2_4920 and Pf6N2E2_4921 are separated by 16 nucleotidesPf6N2E2_4921 and Pf6N2E2_4922 are separated by 301 nucleotidesPf6N2E2_4922 and Pf6N2E2_4923 overlap by 4 nucleotides Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457 _4919 Pf6N2E2_4920 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 5,674,504 to 5,675,169 _4920 Pf6N2E2_4921 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 5,675,186 to 5,676,277 _4921 Pf6N2E2_4922 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 5,676,579 to 5,677,055 _4922 Pf6N2E2_4923 - Transcription termination protein NusB, at 5,677,052 to 5,677,552 _4923 Position (kb) 5675 5676 5677Strain fitness (log2 ratio) -2 -1 0 1at 5674.453 kb on + strandat 5674.453 kb on + strandat 5674.453 kb on + strandat 5674.453 kb on + strandat 5674.454 kb on - strandat 5674.454 kb on - strandat 5674.454 kb on - strandat 5674.454 kb on - strandat 5674.455 kb on + strandat 5674.455 kb on + strandat 5675.155 kb on + strandat 5675.197 kb on + strandat 5675.197 kb on + strandat 5675.197 kb on + strandat 5675.198 kb on - strandat 5675.198 kb on - strandat 5675.479 kb on + strand, within Pf6N2E2_4921at 5675.798 kb on - strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.140 kb on + strand, within Pf6N2E2_4921at 5676.190 kb on + strandat 5676.223 kb on + strandat 5676.223 kb on + strandat 5676.255 kb on + strandat 5676.260 kb on + strandat 5676.260 kb on + strandat 5676.260 kb on + strandat 5676.260 kb on + strandat 5676.260 kb on + strandat 5676.260 kb on + strandat 5676.298 kb on + strandat 5676.298 kb on + strandat 5676.298 kb on + strandat 5676.298 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.319 kb on + strandat 5676.398 kb on + strandat 5676.510 kb on + strandat 5676.510 kb on + strandat 5676.522 kb on + strandat 5676.522 kb on + strandat 5676.522 kb on + strandat 5676.522 kb on + strandat 5676.522 kb on + strandat 5676.525 kb on + strandat 5676.525 kb on + strandat 5676.544 kb on + strandat 5676.564 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vanillyl Alcohol 0.005 M
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5,674,453 + +0.3
5,674,453 + +0.0
5,674,453 + +0.0
5,674,453 + +1.1
5,674,454 - +1.2
5,674,454 - +0.6
5,674,454 - -0.0
5,674,454 - -0.3
5,674,455 + +1.0
5,674,455 + +0.3
5,675,155 + -0.9
5,675,197 + +0.5
5,675,197 + +0.1
5,675,197 + +0.4
5,675,198 - +1.1
5,675,198 - -0.3
5,675,479 + Pf6N2E2_4921 0.27 -1.7
5,675,798 - Pf6N2E2_4921 0.56 +0.0
5,676,140 + Pf6N2E2_4921 0.87 +1.1
5,676,140 + Pf6N2E2_4921 0.87 -0.8
5,676,140 + Pf6N2E2_4921 0.87 -0.6
5,676,140 + Pf6N2E2_4921 0.87 -2.3
5,676,140 + Pf6N2E2_4921 0.87 -0.7
5,676,140 + Pf6N2E2_4921 0.87 -0.5
5,676,190 + -0.4
5,676,223 + -0.7
5,676,223 + +0.7
5,676,255 + +0.1
5,676,260 + +0.1
5,676,260 + -0.0
5,676,260 + -0.7
5,676,260 + +0.5
5,676,260 + -0.9
5,676,260 + -0.3
5,676,298 + -0.3
5,676,298 + +1.0
5,676,298 + -0.6
5,676,298 + +0.5
5,676,319 + +0.1
5,676,319 + +0.1
5,676,319 + -0.6
5,676,319 + -0.3
5,676,319 + +1.3
5,676,319 + -2.3
5,676,319 + -1.2
5,676,398 + -2.3
5,676,510 + -0.1
5,676,510 + -1.0
5,676,522 + +0.5
5,676,522 + +0.8
5,676,522 + -0.7
5,676,522 + +0.1
5,676,522 + -0.7
5,676,525 + -0.6
5,676,525 + +0.8
5,676,544 + -0.0
5,676,564 + -0.8

Or see this region's nucleotide sequence