Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1326

Experiment: Vanillyl Alcohol 0.005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1324 and Pf6N2E2_1325 are separated by 123 nucleotidesPf6N2E2_1325 and Pf6N2E2_1326 overlap by 41 nucleotidesPf6N2E2_1326 and Pf6N2E2_1327 are separated by 292 nucleotides Pf6N2E2_1324 - Transcriptional regulator, AraC family, at 1,557,386 to 1,558,348 _1324 Pf6N2E2_1325 - FIG00954773: hypothetical protein, at 1,558,472 to 1,559,077 _1325 Pf6N2E2_1326 - Cationic amino acid transporter, at 1,559,037 to 1,560,536 _1326 Pf6N2E2_1327 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 1,560,829 to 1,562,157 _1327 Position (kb) 1559 1560 1561Strain fitness (log2 ratio) -2 -1 0 1 2at 1558.098 kb on + strand, within Pf6N2E2_1324at 1558.157 kb on + strand, within Pf6N2E2_1324at 1558.369 kb on - strandat 1558.369 kb on - strandat 1558.403 kb on + strandat 1558.404 kb on - strandat 1558.421 kb on - strandat 1558.551 kb on + strand, within Pf6N2E2_1325at 1558.552 kb on - strand, within Pf6N2E2_1325at 1558.552 kb on - strand, within Pf6N2E2_1325at 1558.756 kb on + strand, within Pf6N2E2_1325at 1559.218 kb on + strand, within Pf6N2E2_1326at 1559.218 kb on + strand, within Pf6N2E2_1326at 1559.218 kb on + strand, within Pf6N2E2_1326at 1559.218 kb on + strand, within Pf6N2E2_1326at 1559.219 kb on - strand, within Pf6N2E2_1326at 1559.219 kb on - strand, within Pf6N2E2_1326at 1559.527 kb on + strand, within Pf6N2E2_1326at 1559.528 kb on - strand, within Pf6N2E2_1326at 1559.528 kb on - strand, within Pf6N2E2_1326at 1559.570 kb on - strand, within Pf6N2E2_1326at 1560.236 kb on - strand, within Pf6N2E2_1326at 1560.289 kb on + strand, within Pf6N2E2_1326at 1560.290 kb on - strand, within Pf6N2E2_1326at 1560.295 kb on + strand, within Pf6N2E2_1326at 1560.295 kb on + strand, within Pf6N2E2_1326at 1560.296 kb on - strand, within Pf6N2E2_1326at 1560.567 kb on + strandat 1560.568 kb on - strandat 1560.568 kb on - strandat 1560.800 kb on + strandat 1560.800 kb on + strandat 1560.800 kb on + strandat 1560.800 kb on + strandat 1560.801 kb on - strandat 1560.801 kb on - strandat 1560.808 kb on + strandat 1560.846 kb on + strandat 1560.917 kb on - strandat 1560.998 kb on - strand, within Pf6N2E2_1327at 1560.998 kb on - strand, within Pf6N2E2_1327at 1561.041 kb on + strand, within Pf6N2E2_1327at 1561.041 kb on + strand, within Pf6N2E2_1327at 1561.042 kb on - strand, within Pf6N2E2_1327at 1561.042 kb on - strand, within Pf6N2E2_1327at 1561.046 kb on - strand, within Pf6N2E2_1327at 1561.046 kb on - strand, within Pf6N2E2_1327at 1561.357 kb on + strand, within Pf6N2E2_1327at 1561.501 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.534 kb on + strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.535 kb on - strand, within Pf6N2E2_1327at 1561.536 kb on + strand, within Pf6N2E2_1327

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Per-strain Table

Position Strand Gene LocusTag Fraction Vanillyl Alcohol 0.005 M
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1,558,098 + Pf6N2E2_1324 0.74 +0.6
1,558,157 + Pf6N2E2_1324 0.80 +0.2
1,558,369 - -2.3
1,558,369 - +0.2
1,558,403 + +0.4
1,558,404 - -0.2
1,558,421 - +0.7
1,558,551 + Pf6N2E2_1325 0.13 +0.5
1,558,552 - Pf6N2E2_1325 0.13 +1.2
1,558,552 - Pf6N2E2_1325 0.13 +0.4
1,558,756 + Pf6N2E2_1325 0.47 +0.1
1,559,218 + Pf6N2E2_1326 0.12 -1.4
1,559,218 + Pf6N2E2_1326 0.12 +0.7
1,559,218 + Pf6N2E2_1326 0.12 +0.4
1,559,218 + Pf6N2E2_1326 0.12 -1.1
1,559,219 - Pf6N2E2_1326 0.12 +1.1
1,559,219 - Pf6N2E2_1326 0.12 +0.6
1,559,527 + Pf6N2E2_1326 0.33 +0.4
1,559,528 - Pf6N2E2_1326 0.33 +2.0
1,559,528 - Pf6N2E2_1326 0.33 -2.5
1,559,570 - Pf6N2E2_1326 0.36 +0.3
1,560,236 - Pf6N2E2_1326 0.80 +0.5
1,560,289 + Pf6N2E2_1326 0.83 -0.9
1,560,290 - Pf6N2E2_1326 0.84 +0.8
1,560,295 + Pf6N2E2_1326 0.84 +1.4
1,560,295 + Pf6N2E2_1326 0.84 +1.4
1,560,296 - Pf6N2E2_1326 0.84 +0.1
1,560,567 + +0.3
1,560,568 - +0.7
1,560,568 - -0.0
1,560,800 + +1.4
1,560,800 + -1.0
1,560,800 + -0.3
1,560,800 + -0.0
1,560,801 - -0.1
1,560,801 - -0.1
1,560,808 + -0.0
1,560,846 + -0.2
1,560,917 - +0.0
1,560,998 - Pf6N2E2_1327 0.13 -0.3
1,560,998 - Pf6N2E2_1327 0.13 -0.5
1,561,041 + Pf6N2E2_1327 0.16 +0.5
1,561,041 + Pf6N2E2_1327 0.16 -0.7
1,561,042 - Pf6N2E2_1327 0.16 +0.3
1,561,042 - Pf6N2E2_1327 0.16 -2.0
1,561,046 - Pf6N2E2_1327 0.16 +2.0
1,561,046 - Pf6N2E2_1327 0.16 +1.1
1,561,357 + Pf6N2E2_1327 0.40 -0.4
1,561,501 + Pf6N2E2_1327 0.51 -2.3
1,561,534 + Pf6N2E2_1327 0.53 +2.5
1,561,534 + Pf6N2E2_1327 0.53 -1.5
1,561,534 + Pf6N2E2_1327 0.53 +0.5
1,561,534 + Pf6N2E2_1327 0.53 -0.2
1,561,534 + Pf6N2E2_1327 0.53 +0.0
1,561,534 + Pf6N2E2_1327 0.53 -0.0
1,561,534 + Pf6N2E2_1327 0.53 -0.4
1,561,534 + Pf6N2E2_1327 0.53 -0.2
1,561,535 - Pf6N2E2_1327 0.53 -0.9
1,561,535 - Pf6N2E2_1327 0.53 -0.4
1,561,535 - Pf6N2E2_1327 0.53 +0.4
1,561,535 - Pf6N2E2_1327 0.53 -1.5
1,561,535 - Pf6N2E2_1327 0.53 -0.5
1,561,535 - Pf6N2E2_1327 0.53 +0.5
1,561,536 + Pf6N2E2_1327 0.53 +0.8

Or see this region's nucleotide sequence