Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1

Experiment: Vanillyl Alcohol 0.005 M

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf6N2E2_1 and Pf6N2E2_2 are separated by 145 nucleotides Pf6N2E2_1 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 90 to 860 _1 Pf6N2E2_2 - FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis, at 1,006 to 1,740 _2 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1at 0.027 kb on + strandat 0.028 kb on - strandat 0.035 kb on - strandat 0.035 kb on - strandat 0.158 kb on - strandat 0.181 kb on + strand, within Pf6N2E2_1at 0.182 kb on - strand, within Pf6N2E2_1at 0.193 kb on + strand, within Pf6N2E2_1at 0.298 kb on + strand, within Pf6N2E2_1at 0.349 kb on + strand, within Pf6N2E2_1at 0.353 kb on - strand, within Pf6N2E2_1at 0.353 kb on - strand, within Pf6N2E2_1at 0.369 kb on + strand, within Pf6N2E2_1at 0.370 kb on - strand, within Pf6N2E2_1at 0.370 kb on - strand, within Pf6N2E2_1at 0.838 kb on + strandat 0.839 kb on - strandat 0.839 kb on - strandat 0.839 kb on - strandat 0.902 kb on + strandat 0.902 kb on + strandat 0.902 kb on + strandat 0.903 kb on - strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.905 kb on - strandat 0.905 kb on - strandat 0.948 kb on - strandat 0.955 kb on + strandat 0.955 kb on + strandat 1.037 kb on - strandat 1.060 kb on + strandat 1.060 kb on + strandat 1.060 kb on + strandat 1.061 kb on - strandat 1.349 kb on - strand, within Pf6N2E2_2at 1.430 kb on + strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Vanillyl Alcohol 0.005 M
remove
27 + -0.4
28 - -0.4
35 - +0.5
35 - -1.4
158 - +0.2
181 + Pf6N2E2_1 0.12 -0.9
182 - Pf6N2E2_1 0.12 +0.2
193 + Pf6N2E2_1 0.13 -0.4
298 + Pf6N2E2_1 0.27 -1.0
349 + Pf6N2E2_1 0.34 -1.7
353 - Pf6N2E2_1 0.34 -0.8
353 - Pf6N2E2_1 0.34 -0.4
369 + Pf6N2E2_1 0.36 -0.9
370 - Pf6N2E2_1 0.36 -0.6
370 - Pf6N2E2_1 0.36 -0.2
838 + -0.1
839 - +0.7
839 - -0.8
839 - -0.2
902 + +0.3
902 + +0.2
902 + +0.6
903 - +0.7
904 + -0.4
904 + +1.0
904 + -0.7
904 + -0.6
905 - +0.6
905 - -0.5
948 - -0.3
955 + +0.5
955 + -1.6
1,037 - +0.4
1,060 + +0.1
1,060 + -0.2
1,060 + -0.7
1,061 - +0.5
1,349 - Pf6N2E2_2 0.47 +0.1
1,430 + Pf6N2E2_2 0.58 -0.0
1,431 - Pf6N2E2_2 0.58 -0.4
1,431 - Pf6N2E2_2 0.58 +1.4
1,431 - Pf6N2E2_2 0.58 +0.8

Or see this region's nucleotide sequence